肺リンパ管筋腫症の1例

Article信州医学雑誌 46(2): 135-139(1998)journal articl

長野県内おける呼吸器内科医の勤務状況に関する調査 : 特に上小地域と他地域との比較

A shortage of doctors is now an urgent problem on the medical scene in Japan.The current situation of pulmonary doctors in Nagano was analyzed by a questionnaire survey,in which we also estimated the present state of pulmonary medicine in the Jo-Sho region. We sent out a questionnaire asking about the number of pulmonary physicians, the number of sickbeds, and the types of pulmonary disease patients in nineteen institutions affiliated with our institution. We also evaluated the relative status of pulmonary medicine at the Shinshu-Ueda Medical Center (formerly the National Nagano Hospital). Thirteen institutions responded to the survey.Ten out of the thirteen institutions felt there is a shortage of pulmonary physicians. The number of patients with pulmonary disease was significantly larger in large institutions (with more than 300 sickbeds).Although the pulmonary division of Shinshu-Ueda Medical Center specializes in pulmonary medicine (95% of inpatients in this division had pulmonary problems), the doctorpatient ratio was the highest among all the large institutions.Article信州医学雑誌 59(6): 419-422(2011)departmental bulletin pape

えのき栽培従事者に発症した培地が原因と考えられた過敏性肺臓炎の１例

We report a case of hypersensitivity pneumonitis in a worker cultivating enoki mushrooms.The patient developed symptoms of cough, sputum, and dyspnea after changing the culture medium of the enoki mushrooms. Radiographic findings showed small nodules and ground glass opacity in both lung fields. Bronchoalveolar lavage revealed a marked increase in lymphocytes.Lymphocyte stimulation test in both blood and bronchoalveolar lavage were positive for the culture medium but not for enoki mushrooms. Based on the results, a diagnosis of hypersensitivity pneumonitis caused by the culture medium of enoki mushrooms was confirmed. We should consider that the culture medium is a possible antigen leading to hypersensitivity pneumonitis.Article信州医学雑誌 53(2): 73-75(2005)journal articl

利用多组学手段解析番茄育种过程中代谢物变化的机制

番茄(Solanum lycopersicum)在育种过程中经历了驯化、改良、分化和渐渗等不同阶段,在这一选择过程中番茄的果重和风味等均发生了显著改变,但是目前对于番茄育种过程中代谢物的变化及其遗传基础却不是十分清楚。近期,中国农业科学院深圳农业基因组研究所黄三文研究组与华中农业大学罗杰研究组利用多组学(变异组、转录组及代谢组)手段系统解析了番茄育种过程中代谢物的变化。结果表明,在番茄驯化过程中有46个甾醇糖基生物碱类物质(SGAs)含量逐渐降低,并获得了7个与其中44个物质显著相关的遗传位点。因此,在番茄育种过程中通过优异等位位点的组合可以显著降低SGAs的含量;同时发现在番茄以果重为目标的选择过程中,控制果重基因周围其它基因的"搭车效应"是引起许多代谢物变化的重要遗传因素,及在育种过程中对某一性状的选择会对其它性状产生重要影响。该研究首次利用多组学手段系统解析了选择对作物代谢物的影响,为番茄品质改良奠定了良好的理论基础

基于GC-MS的植物代谢组学研究

代谢组学是系统生物学的重要组成部分。在众多代谢组学分析技术中,气相色谱-质谱联用(GC-MS)技术较为成熟,分辨率高、灵敏度高、重现性好,拥有大量标准质谱图数据库且成本相对低廉,很早就应用到植物代谢组学研究领域,迄今仍然是主要分析平台之一。现从GC-MS技术的核心原理、衍生化方法以及数据采集、分析和数据库等方面介绍了GC-MS植物代谢组学分析技术。同时,综述了该技术在基因功能研究、植物代谢遗传机理、植物抗逆、生物技术和生物育种中的应用

植物代谢组学数据分析和数据库

植物代谢组学是近年发展起来的进行植物生物学研究的新兴学科,目前的难点和热点是数据分析和信息挖掘。现针对植物代谢组学数据分析和信息挖掘过程中涉及的几个主要方面进行了概述,内容包括数据特点、分析流程、软件和数据库。系统地介绍了植物代谢组数据分析预处理、常用统计分析原理和适用条件、数据分析中的注意事项、常用软件和数据库的优缺点,最后探讨了植物代谢组数据分析的问题和新发展动态

大麦抗叶锈病慢锈性鉴定技术及抗性评价方法

成功的病原菌接种和准确的病情调查是大麦(Hordeum vulgare)抗病研究的基础。该文总结了大麦抗叶锈病慢锈性鉴定实验中几种常用的喷雾和涂抹接种方法,以及主要的抗性评价指标,并对操作过程中的一些注意事项进行了说明