35 research outputs found
Research on the Skin Effect of Carbon Fiber Composites
良导体在平面电磁场作用下的趋肤效应有简单的公式描述,而涡流检测中电各向异性材料的趋肤效应却非常复杂。该文通过对碳纤维复合材料的涡流检测进行有限元仿真,研究涡流检测中电各向异性材料的趋肤效应。The skin effect of anisotropic material is much more complex than that of good conductors which is described by a simple formula.In the article,we will explore the skin effect of anisotropic material by simulating the eddy current testing ofcarbon fiber composites with finite elementmethod
基于分层虚拓扑的跨粒度层增强生存性的方法
在多粒度光网络中,为了满足可变的业务带宽请求,降低高带宽请求业务的阻塞率,减少低带宽请求业务接入时造成的网络资源浪费,提出了一种基于分层虚拓扑的跨粒度层增强生存性的方法。该方法首先根据波长粒度和波带粒度设计了一种分层虚拓扑,然后根据业务请求带宽在各粒度层上或跨粒度层计算工作路径和保护路径。分析结果表明,该方法可有效提高资源利用率,降低阻塞率
Normalized model of a 3-parameter isolation system and its parametric optimization
三参数隔振系统较传统两参数隔振系统在共振峰和高频段表现更加优异。提出归一化的三参数隔振系统设计方法,给出了正弦激励以及阶跃激励下三参数隔振系统的时域响应解析形式。针对正弦激励,以共振峰和高频衰减率作为目标函数,进行多目标优化设计,针对单位阶跃激励,以调节时间和超调量为目标函数进行参数设计,相比于传统设计方法,能够给出同时满足目标函数的解,仿真算例验证了方法的可行性
Cloning of cDNA 5’End of Gonad Differential Expression Gene GD13 in Penaeus japonicus
采用基于SMART(SwitchingMechanismAt5'endofRNATranscript)原理的cDNA末端快速扩增技术(RapidAmpli ficationofcDNAends:RACE),对日本对虾性腺差异表达基因cDNA5'端进行克隆,即在逆转录过程中通过SMARTⅡoligo的介导,在cDNA第1条链的3'端接上接头,然后根据已知序列设计的基因特异性引物(GSP1和GSP2)和接头引物(UPM和NUP)进行Nested PCR特异性扩增,并对特异产物进行克隆和序列分析,最终获得全长cDNA序列.测序结果表明,在卵巢内表达量高于精巢的GD13基因全长为656bp,编码164氨基酸,经NCBI检索,此基因与文昌鱼、家鼠的核糖体蛋白L24及黄牛的核糖体蛋白L30氨基酸序列同源性分别高达58%、56%和56%,可断定该基因为编码日本对虾核糖体蛋白L24亚基的基因.为进一步认识对虾生长发育和繁殖的调控机制奠定基础.In this paper,the method of SMART Rapid Amplification of cDNA ends (RACE) was employed to clone the 5’ end of cDNA of GD13,which is a gene differentially expressed between ovary and testis of Penaeus japonicus.The SMARTII Oligo nucleotide was used as a template to stretch the cDNA 5’end when the first strand cDNA was synthesized.Then a Nested-PCR was carried out to specially amplify the interested cDNA with the adaptor primers (UPM and NUP) and the gene special primers (13GSP1 and 13GSP2).As a result,we got a 656 bp full length cDNA of GD13 which encodes 164 amino acid.Homologous comparison of amino acid sequence demonstrated that this gene shared significant homology with the ribosome protein L24 gene of Branchiostoma belcheri,Mus musculus and L30 gene of Bos Taurus.Further work investigating why this ribosome protein gene differentially expressed in testis and ovary of shrimp is going on.国家自然科学基金(30070597);; 国家骨干教师基金资
