液相色谱-串联质谱前驱离子扫描非靶向筛查卤代有机物

建立了液相色谱-串联质谱(LC-MS/MS)前驱离子扫描(PIS)非靶向筛查卤代有机物的分析方法。卤代有机物在PIS分析中产生的卤素碎片离子能通过PIS Q3预设的质核比通道(m/z 35、37、79、81和127),根据通过的质核比通道可判断卤代有机物种类,并获取其分子离子质核比信息,而非卤代有机物无法通过PIS Q3的筛选。当Q2碰撞能为50 eV时,PIS对卤代有机物具有较高的灵敏度。利用伯努利概型总结了不同卤代有机物的理论同位素峰数量和丰度比的规律,结合PIS分析结果可判断有机物携带卤素原子数量。本方法成功应用于实验室样品和海水中卤代有机物的筛查,未来有望用于其它环境样品中卤代有机物的快速筛检。国家自然科学基金项目(Nos.21477115,21876142)资助~

Study on the Xiamen University 2013 Solar Decathlon Energy Saving System

国际太阳能十项全能竞赛,倡导将太阳能利用与建筑节能先进技术紧密结合,设计、建造并运行一座功能完善、舒适、宜居、可持续性的太阳能居住空间。该文探讨了厦门大学参赛方案节能体系配置,重点介绍其太阳能光热与光电系统、围护结构材料、空调系统、相变通风及智能控制系统等方面的节能举措。国际太阳能十项全能竞赛设计作为一个系统工程,对太阳能利用与建筑节能进行一体化设计、协调配合才能使参赛方案取得预期的节能成效。Solar Decathlon is aimed at integrating solar applications with building energy-saving advanced technology closely,all teams were asked to design,build and run a fully functional,comfortable,livable,and sustainable solar living space.This paper discusses the energy saving system of the project from Xiamen University.It focuses on its solar thermal and photovoltaic systems,building envelope materials,air conditioning systems,intelligent controlled PCM ventilation systems and other aspects of energy-saving initiatives.The competition requires a systemic point of view that the solar energy application and building energy saving design should be well integrated with.Only in this way can make the project to achieve the expected energy saving effect

Emodin induces apoptosis of cancer cells and inhibits retinoid X receptor transcriptional activity

大黄素(emodin)对多种肿瘤细胞有较强的抑制增殖和诱导凋亡的作用,但其作用机制尚不清楚。本研究通过配体-受体竞争结合实验以及报告基因检测了大黄素对维甲酸X受体(retinoid X receptor alpha,RXRα)的结合和转录活性的调控,并研究了大黄素对肺癌细胞H460和肝癌细胞SMMC-7721生长和凋亡的作用。结果发现,大黄素对两种癌细胞有很强的抑制增殖作用,加入RXRα的天然配体9-顺式视黄酸(9-cis-retinoid acid,9-cis-RA)共同处理可显著缓解这种抑制作用。大黄素能浓度依赖地引起两种癌细胞系的凋亡,使细胞核出现碎裂和染色质浓染。报告基因实验发现大黄素对RXRα同源和异源二聚体的转录激活有显著抑制作用。体外的配体竞争结合实验发现,大黄素不直接结合RXRα的配体结合区。蛋白质免疫印迹实验发现,大黄素不影响RXRα的蛋白表达。结果提示,大黄素具有诱导肺癌细胞H460和肝癌细胞SMMC-7721凋亡和抑制细胞生长的作用,大黄素抑制9-cis-RA对RXR转录激活作用以及9-cis-RA具有一定程度拮抗大黄素对肺癌细胞H460和肝癌细胞SMMC-7721的生长抑制作用,提示大黄素的抗癌作用可能与细胞内RXR的功能有关,并以RXR转录非依赖性的方式起作用。配体竞争结合实验结果提示大黄素可能间接作用于RXR。The mechanisms by which emodin induces apoptosis and inhibits proliferation of cancer cells remain unclear.In this study,we investigated whether the proapoptotic effect of emodin on human NIH-H460 lung cancer cells and SMMC-7721 liver cancer cells was related to regulating RXR expression and function.MTT assay and DAPI staining were used to detect the anti-proliferative and apoptotic effects of emodin with or without 9-cis-retinoid acid on H460 and SMMC-7721.The reporter assay was used to detect the effect of emodin on RXR homo-and hetero-dimer transactivation.Competitive ligand binding assay was carried out to detect whether emodin could directly bind to RXR.The result showed that emodin could strongly inhibit the proliferation and induce apoptosis of both cancer cell lines,which could be antagonized by 9cis-RA.The reporter assay showed that emodin could inhibit the transcriptional effect of the homo-and hetero-dimer transactivation of RXRα dose-dependently.However,in vitro binding assay did not show that emodin bind to RXRα-LBD directly.The findings suggest that exhibition of emodin its anti-cancer activity may be associated with involvement of RXRα signal transduction pathways.国家高技术研究发展计划(863计划)资助项目(2007AA09Z404);; 国家自然科学基金资助项目(30471939);; 福建省重点资助项目(2007I01021026);; 上海市科委重大科技攻关项目(OZ041915);; 上海市优秀学科带头人项目(06XD14021)

Progress on Fabrication, Modification and Applications of Titania Nanotube Arrays

新型纳米材料TiO2纳米管阵列具有独特的、高度有序的阵列结构和良好的力学性能、化学稳定性以及抗腐蚀性能。该材料以纯金属钛为基体,在含有少量氟离子的电解质溶液中通过电化学阳极氧化法制得。本文综述了近年来TiO2纳米管阵列在不同电解液体系中的制备工艺、形成机理、修饰改性及其在光催化降解污染物、太阳能电池、气敏传感材料、光解水制氢等领域应用的最新研究成果,并指出目前存在的问题,对今后的研究提出了展望。Titania nanotube arrays as a novel nano-material has unique highly ordered array structure, good mechanical and chemical stability, as well as excellent corrosion resistance. It has been fabricated by electrochemical anodization of pure Ti sheet in electrolytes containing small amounts of fluoric ions. This paper reviews the new research achievements of TiO2 nanotube arrays on the preparation processes, forming mechanism, modification and the applications in the photocatalytic degradation of pollutants, solar cells, gas sensor materials, photolysis water. The existing problems and further prospects in this field are also discussed.福建省自然科学基金资助项目(No.2007J0227

Progress on Fabrication, Modification and Applications of Titania Nanotube Arrays

第一作者: 孙岚, 女, 41 岁, 博士, 副教授; 研究方向: 纳米材料的制备及其应用。[中文文摘]新型纳米材料TiO2 纳米管阵列具有独特的、高度有序的阵列结构和良好的力学性能、化学稳定性以及抗腐蚀性能。该材料以纯金属钛为基体, 在含有少量氟离子的电解质溶液中通过电化学阳极氧化法制得。本文综述了近年来TiO2 纳米管阵列在不同电解液体系中的制备工艺、形成机理、修饰改性及其在光催化降解污染物、太阳能电池、气敏传感材料、光解水制氢等领域应用的最新研究成果, 并指出目前存在的问题, 对今后的研究提出了展望。[英文文摘]Titania nanotube arrays as a novel nano-material has unique highly ordered array structure, good mechanical and chemical stability, as well as excellent corrosion resistance. It has been fabricated by electrochemical anodization of pure Ti sheet in electrolytes containing small amounts of fluoric ions. This paper reviews the new research achievements of TiO2 nanotube arrays on the preparation processes, forming mechanism, modification and the applications in the photocatalytic degradation of pollutants, solar cells, gas sensor materials,photolysis water. The existing problems and further prospects in this field are also discussed.福建省自然科学基金资助项目(No.2007J0227)。通讯联系人。E-mail: [email protected]

Green Synthesis of Potassium Diformate

以甲酸和氢氧化钾为原料,不外加任何溶剂一步合成二甲酸钾。通过单因素实验,考察了原料配比、反应温度和反应时间对产品质量与收率的影响。采用正交实验进一步优化了合成工艺,确定了最佳工艺条件:n(HCOOH)/n(KOH)=2.2、反应温度70℃、反应时间30 m in,在该条件下,二甲酸钾产品收率达98%以上。所得产品经红外光谱分析及熔点测定,其结果与文献报道一致。反应过程绿色化,有很好的原子经济性。Potassium diformate was synthesized without solvent,using formic acid and potassium hydroxide as raw materials.The optimal conditions for the reaction were:molar ratio of HCOOH to KOH 2.2∶1,reaction temperature 70 ℃ and reaction time 30 min.Yield of the process was over 98% under the optimal conditions.The infrared spectrum and melting range of the product were in good agreement with those reported in literature.The technology developed in this study could be an environmentally benign synthesis route with efficient atom economy

The protective effects of compound sarcopyramis nepalensis wall on acute hepatic injury in mice

目的观察复方风柜斗草对四氯化碳(CCl4)引起的小鼠急性肝损伤的保护作用。方法将小鼠随机分为空白组、模型组、复方风柜斗草低剂量组(2.2 g/kg)、中剂量组(4.4 g/kg)、高剂量组(8.8 g/kg)和联苯双酯阳性对照组(0.2g/kg)。空白组与模型组给予1%羧甲基纤维素钠(CMC-nA)水溶液,其他组给予相应的药物,1次/d,连续灌胃给药10 d后,除正常组外,腹腔注射CCl4致小鼠急性肝损伤。检测肝组织中的超氧化物歧化酶(SOd)及血清中丙氨酸氨基转移酶(AlT)、天冬氨酸氨基转移酶(AST)的活性,同时检测血清中总胆红素(T-bIl)、直接胆红素(d-bIl)及肝组织中丙二醛(MdA)的水平,并计算肝脏指数。结果预先给予复方风柜斗草能显著降低急性肝损伤小鼠体内T-bIl、d-bIl、AlT、AST和MdA的水平,并升高肝组织中SOd活力。结论复方风柜斗草对CCl4致小鼠急性肝损伤具有保护作用,其机制可能与其所具有的抗脂质过氧化和清除体内过多氧自由基的作用有关。Objective To observe the protective effects of compound sarcopyramis nepalensis wall against tetrachloride( CCl4)-induced acute hepatic injury in mice.Methods The mice were randomly divided into normal group,model group,low dose compound sarcopyramis nepalensis wall group( 2.2 g / kg),medium dose group( 4.4 g / kg),high dose group( 8.8 g / kg),and positive control( biphenyldicarboxylate) groups( 0.2 g / kg).The mice were ig pretreated with compound sarcopyramis nepalensis wall and biphenyldicarboxylate once daily for consecutive 10 d,respectively.The mice in the normal and model groups were given 0.1% CMCNa,the water-solution instead.Then,acute liver injury in mice was induced by ip injection of CCl4.The activities of serum ALT and AST,hepatic SOD,and the level of serum T-BIL and D-BIL,hepatic MDA were also determined,the hepatic indices were calculated.Results The activities of ALT and AST,the level of T-BIL,D-BIL and MDA were significantly decreased by compound sarcopyramis nepalensis wall pretreatment,while activity of SOD in hepatic tissues was markedly increased.Conclusion Compound sarcopyramis nepalensis wall has protective effects against CCl4-induced acute hepatic injury in mice,and the mechanisms might be associated with its anti-oxidative and scavenging free radical activity

Effects of Aqueous Extracts of Coptis Chinensis Franch and Prunus Mume on Intestinal Bacteria in Vitro

通过滤纸片法初步测定了黄连和乌梅水提液的抗菌性能,用比浊法进一步考察了它们对5种肠道菌体外生长的影响。结果表明,0.001 g/mL黄连和乌梅水提液均能够有效抑制产气荚膜梭菌和金黄色葡萄球菌的生长,轻微促进干酪乳杆菌的生长,但对嗜酸乳杆菌的生长只有微弱的抑制作用。0.01 g/mL黄连水提液对大肠杆菌的生长影响不大。而0.001 g/mL乌梅水提液可促进大肠杆菌的生长,但当质量浓度升高至0.005 g/mL时,则对大肠杆菌的生长表现出抑制作用。因此,黄连、乌梅水提液可能是潜在的益生元,是功能性食品配料、绿色饲料添加剂的候选者。The effects of aqueous extracts from C.chinensis Franch root(Coptis root)-derived and P.mume fruit-derived materials on the growth of intestinal bacteria were examined firstly by using impregnated paper disk method,and further by using spectrophotometric method.Both Coptis root and P.mume extracts exhibited potent inhibition against Clostridium perfringens and Staphylococcus aureus at mass concentration as low as 0.001 g/mL,whereas only weak inhibitory activity was detected against Lactobacillus acidophilus.Moreover,growth of Lactobacillus casei was slightly promoted by both extracts at the same mass concentration.No inhibition against Escherichia coli was observed from treatment with Coptis root extracts even at 0.01 g/mL.On the contrary,P.mume extracts showed slight stimulation effects on the growth of E.coli at 0.001 g/mL,but exhibited significant growth inhibition at 0.005 g/mL.These results indicated that Coptis extracts and P.mume extracts may be potential prebiotics,and could be alternatives for functional food additives and green feed additives