Chemical Constituents in Chloroform Extraction of Tetrastigmatis hemsleyani Diels et. Gilg and Their Antitumor Activities

目的研究三叶青三氯甲烷部位的化学成分及其体外抗肿瘤活性。方法利用硅胶,制备薄层板,lH-20等各种色谱技术分离单体化合物,通过nMr和质谱对化合物进行结构鉴定,并采用气-质联用色谱对三叶青三氯甲烷部位的其他成分进行分析,采用MTT法对分离得到的单体化合物进行抗肿瘤活性评价。结果通过经典色谱法分离得到6个化合物,分析鉴定为β-谷甾醇(1),4-羟基-3-甲氧基苯甲醛(2),齐墩果酸(3),5-羟甲基糠醛(4),壬二酸(5)和香草酸(6),并通过gC-MS指认了22个化合物。结论化合物2~6首次从三叶青中分离得到。化合物1和3对HElA229有较强的细胞毒活性,IC50分别为40.78,25.69μg·M l-1。化合物3对A375有较强的细胞毒活性,IC50为69.87μg·M l-1。三叶青三氯甲烷部位的体外抗肿瘤活性由多种成分共同作用产生。OBJECTIVE To investigate the chemical constituents in chloroform extraction of Tetrastigmatis hemsleyani diels et.Gilg and their antitumor activities.METHODS Various chromatography techniques such as column chromatography on silica gel,Sephadex LH-20 and preparative TLC were used to isolate and purify the compounds.Their structures were identified by1H-NMR,13C-NMR and MS.Their antitumor activities was tested by MTT method.Moreover,the other compounds of chloroform extraction were detected by GC-MS.RESULTS Six compounds were isolated by classic chromatography and identified as β-sitosterol( 1),4-hydroxy-3-methoxybenzaldehyde( 2),oleanolic acid( 3),5-hydroxymethyl furfural( 4),azelaic acid( 5),vanillic acid( 6).Twenty-two compounds were identified by GC-MS.CONCLUSION Compounds 2-6 are isolated from this plant for the first time.Compounds 1 and3 shows strong cytotoxic activities against Hela229 with IC50 values of 40.78,25.69 μg · m L-1,respectively.Compound 3 also showed strong cytotoxic activities against with IC50 values of 69.87 μg·m L-1.The result proved that antitumor activity of chloroform extraction of Tetrastigmatis hemsleyani diels et.Gilg is due to the contribution of multi-components.宁波市自然科学基金资助项目(2012A610181

Effects of valtrate on anxiety models in rats and its possible mechanisms

目的研究缬草素的抗焦虑作用,并分析其可能的作用机制。方法以大鼠高架十字迷宫实验和开场实验为动物模型,考察缬草素的抗焦虑活性;用酶联免疫吸附法（ELISA）测定大鼠血清皮质酮的水平。结果缬草素（10mg.kg-1）能增加大鼠在开臂内运动时间和次数百分率,并且提高大鼠在开场中央区的次数,在大鼠高架十字迷宫与开场模型上显示出抗焦虑作用;酶联免疫吸附法表明：缬草素能降低大鼠血清皮质酮水平。结论缬草素具有明显的抗焦虑作用,机制可能与调节下丘脑-垂体-肾上腺轴功能有关

Anxiolytic Activity of Valeportiate

目的:研究缬草素的抗焦虑作用。方法:雄性SD大鼠60只,随机分为5组:缬草素高、中、低剂量组(剂量分别为20,10,5 mg·kg-1)、阳性药地西泮(diazepam,DZP)组和空白组,ig 10 d后采用国际通用的高架十字迷宫(elevated plusmaze,EPM)焦虑动物模型,从行为学角度观察了缬草素的抗焦虑作用。结果:EPM行为学观察表明:与空白组相比,DZP和缬草素中剂量组可明显提高大鼠在开臂时间百分率(14.07%,38.09%,37.42%,P<0.01)和次数百分率(16.98%,30.10%,37.19%,P<0.05或P<0.01)。结论:缬草素有明显的抗焦虑作用。Objective：To study anxiolytic activity of valeportriate isolated from the root of Valeriana jatamansi.Method：Sixty Sprague-Dawley rats were randomly divided into 5 groups for daily intragastric administration of drugs：valeportriate high-dose（20 mg·kg^-1）,valeportriate medium-dose（10 mg·kg^-1）,valeportriate low-dose（5 mg·kg^-1）,the positive drug group and normal control group,treated ig for 10 days.Adopting the international common-used anxiety animal model with the elevated plus-maze test（EPM）,we observed the effect of anti-anxiety of valeportiate from the angle of behavior.Result：The results of EPM showed that DZP and valeportriate medium-dose group obviously increased the percentages of open arm time（14.07%,38.09%,37.42%,P 〈 0.01） and entry（16.98%,30.10%,37.19%,P 〈 0.05 or P 〈 0.01） comparing with control group.Conclusion：Valeportriate has obvious anti-anxiety activity

Effects of valtrate on anxiety models in rats and its possible mechanisms

目的研究缬草素的抗焦虑作用,并分析其可能的作用机制。方法以大鼠高架十字迷宫实验和开场实验为动物模型,考察缬草素的抗焦虑活性;用酶联免疫吸附法(ELISA)测定大鼠血清皮质酮的水平。结果缬草素(10mg.kg-1)能增加大鼠在开臂内运动时间和次数百分率,并且提高大鼠在开场中央区的次数,在大鼠高架十字迷宫与开场模型上显示出抗焦虑作用;酶联免疫吸附法表明:缬草素能降低大鼠血清皮质酮水平。结论缬草素具有明显的抗焦虑作用,机制可能与调节下丘脑-垂体-肾上腺轴功能有关

生长氧化锌薄膜材料的方法

一种生长氧化锌薄膜材料的方法，包括：选用硅单晶基片作为生长氧化锌薄膜材料的衬底；选用甲醇作为氧源，二乙基锌作为锌源；将清洗过的硅衬底放在MOCVD设备的反应室衬底底座上；将反应室抽真空后再充氮气升压，如此反复两次，以排净反应室中的空气；充氮气将反应室升压至适合生长的压强；将衬底升温至适合氧化锌生长的温度；打开反应室衬底底座旋转开关，使得衬底匀速旋转；在MOCVD设备中利用载气从衬底侧面通入二乙基锌，在硅衬底上生长一层锌隔离层；在MOCVD设备中利用载气从衬底顶部通入甲醇，使之与锌源反应在锌隔离层上得到氧化锌薄膜外延层；关闭锌源和氧源载气，待反应室压强升至常压，温度降至常温后从MOCVD设备中取出氧化锌样品

数据分析方法在证候要素研究中的应用概况

中医证候要素研究领域常用的数据分析方法有聚类分析、因子分析、回归分析等,结合现有文献,笔者发现这些数据分析方法在临床科研中都有各自的优势和弊端,无法综合全面的反映证候要素的基本特征和复杂的内部联系。因此,通过系统的整理和分析目前应用于中医证候要素研究中的数据分析方法发现,隐结构法是相对更适合于证候要素研究的一种分析方法,不仅最大限度的保留了其他分析方法的优点,也可以对证候要素进行定量化研究,更接近于中医的辨证论治体系。The data analysis methods including cluster analysis, factor analysis, regression analysis are commonly used in the research field of TCM syndrome elements. After studying existing literatures, we find that these data analysis methods have their own advantages and disadvantages in clinical research, in other words, the basic characteristics of complex syndrome elements and internal linkages cannot be comprehensively integrated by these methods. Therefore, through systematic analysis on data analysis methods currently used in the study of TCM syndrome elements, we find that the latent class analysis method is an analytical method that is more suitable for the study of syndrome elements, not only retains advantages of other analytical methods to the greatest extent, but can also be used to quantitatively study the syndrome elements, which accords with the syndrome differentiation system of Chinese medicine

聚｛吡咯-2,5-二[(对二甲氨基)苯甲烯]｝的电化学和原位拉曼光谱

采用电化学循环伏安法（ＣＶ）和原位拉曼光谱（ｉｎ－ｓｉｔｕ Ｒｅｍａｎ）对窄能隙共轭高分子聚 ｛吡咯－２，５－二［（对二 甲氨基）苯甲烯］｝（ＰＰＤＭＡＢＥ）的电化学行为进行了研究．结果表明，在不同ｐＨ值ＮａＮＯ３溶液中，ＰＰＤＭＡＢＥ的 电化学氧化还原过程中存在吡咯环的氧化态结构与芳式和醌式结构之间的转变．聚合物在氧化态时吡咯环主要 以氧化态存在，而还原态以芳式和醌式结构吡咯环为主．ＰＰＤＭＡＢＥ在酸性和中性介质中氧化态吡咯环以质子化 的状态存在，而在碱性溶液中氧化态吡咯环既有质子化态，又有去质子化态的．在酸性条件下，ＰＰＤＭＡＢＥ较易发 生氧化还原反应，而在碱性条件还原反应则较难发生