LOCALIZATION OF ANTIGENS AND ANTIBODIES IN AEDES ALBOPICTUS

[中文摘要]目的　研究白纹伊蚊组织内抗原、抗蚊组织抗体的分布情况。方法　直接法和间接法进行免疫酶组织化学染色。结果　白纹伊蚊的肠管壁两侧、头部组织、马氏管以及卵巢均出现较强的染色反应 ,但肌肉组织与对照组无明显差别。直接法免疫组化染色 ,在白纹伊蚊卵巢小管壁周围出现阳性反应。结论　白纹伊蚊免疫活性抗原主要分布在肠管壁两侧 ,头部 ,马氏管和卵巢等组织中。部分小鼠血清可穿过白纹伊蚊肠壁集中在卵巢小管壁周围。[英文摘要]Aim The distribution of immuno- activated antigens of female Aedes . albopictus and antiserum of anti- Ae. albopictus were studied. Methods Direct and indirect Immunohistochemical peroxidase diaminobenzidine ( DAB) methods. Results Stronger reaction distributed on the midgut wall, ovary , malpighian tubule and head, the reaction of muscle was negative. The positive reaction appeared on the outer of ovary wall by direct immunohistochemical method. Conclusion Immuno- activated antigens located in the midgut wall, ovary, malpighian tubule and head. Some antiserum of anti- Ae. albopictus drilled through the midgut and distributed in the outer of ovary wall

吸血昆虫抗凝血活性物质的研究进展

福建省青年科技人才创新项目基金资助 (2 0 0 2J0 5 1

A new species of macrobothriidae (Cestoda : Diphyllidea) from thornback ray Platyrhina sinensis in China

A new cestode, Macrobothridium sinensis n. sp., is described from the spiral intestine of Platyrhina sinensis from coast of Xiamen, China. It is the first record of the order Diphyllidea in China. The new species can be distinguished from the 3 previously described species of Macrobothridium by the testes number (16-24 vs. 29-37, 27-46, and 5-6, in M. rhynchobati, M. euterpes, and M. syrtensis, respectively) and a single-row arrangement of testes; M. sinensis is most similar to M. euterpes in strobila length (1.77-6.23 vs. 2-4.5 mm) and number of segments (6-8 vs. 5-9). In addition, M. sinensis differs from M. rhynchobati and M. syrtensis in the strobila length, ovary shape, and number of apical hooks. The uterine pore is the first described in the Diphyllidea; thus, the present uterine pore can now be used to distinguish Macrobothridium from Echinobothrium and Ditrachybothridium in the Diphyllidae

Record of a new polycystic echinococcus,Polycystia neimonguensis sp.nov.in Inner Mongolia,China

1999年夏天在内蒙古东北部呼伦贝尔草原的一只长爪沙鼠 (Merionesunguiculatus)肝脏发现一早期的多囊棘球蚴 (polycysticechinococcus)。布满小泡囊的条状虫体其基部牢固地着生在鼠肝脏组织上 ,其余部分游离在鼠肝表面 ,经切片观察其结构系属多囊棘球蚴特征。虫体含外生泡囊 (exogenousvesicles)和内生泡囊 (endogenousvesicles)芽体。各外生泡囊是从虫体胚组织向体外生长出来 ,泡囊间的胚组织中布满PAS阳性颗粒体 (granulesbodies)和内生泡囊芽体 ,在芽体基部具有环状透明层状膜 (laminatedmenbrane)的结构 ;各外生泡囊内壁中也有颗粒和具层状膜的芽体。内蒙古的多囊棘球蚴结构与南美洲的福氏棘球绦虫 (Echinococusvogeli)的多囊棘球蚴相似但不全相同 ,兹暂定名为内蒙古多囊蚴 (Polycystianeimonguensissp nov ) ,该虫是此类人兽共患寄生虫病原在我国的首次发现。An infection of primary polycystic echinococcus was found in the liver of a gerbil,Meriones unguiculatus Milnc Edwards during a survey on the infection of alveolar echinococcus in rodents which was undertaken in 1999 at the Hulunbeier Pasture of northeastern Inner Mongolia,China Of 45 gerbil,one (2 22%) was found infected with primary polycystic echinococcus consisted of many exogenous vesicles on the whole surface of this metacestode The larval cestode situated superficially in a liver lobe of the gerbil,it was not completely surrounded by host liver tissue,it is therefore not difficult to be dissected out total without rupturing it The dissected primary polycystic echinococcus was a short conical body measuring about 7 5mm in length,3mm in width and 2 5mm in thickness Only its base portion with fibrous tissue was attached to gerbil liver tissue and closely surrounded by connective tissue of host The sections of the metacestode showed that all the exogenous vesicles have arisen directly from germinal tissue of the parasite,it happened prior to onset of exogenous proliferation The sizes of exogenous vesicles were 0 141～0 724×0 432～0 752 mm in cross section specimen The base portion of the metacestode consists of fibrous tissue,the exogenous vesicles were also found attaching to the lateral edges of the base portion From the sections of the base portion,many strong PAS positive mass were observed These germinal tissue masses were structurally similar to that observed in the alveolar metacestodes of Echinococcus sibiricensis Rausch and Schiller,1954 and Alveolaris hulunbeierensis Tang et al ,2001 (Tang et al ,2001;2002;etc ) Histochemistry study showed that the germinal tissue between exogenous vesicles and in the internal surface of exogenous vesicles walls had reticular appearances,and were filled with large number of PAS positive granules bodies measuring about 2 3μm in diameters Other spherical or conical buds of early stage endogenous vesicles with different sizes (6～16×6～8μm),and the buds enveloped by laminated membrane were also observed All the granules and small buds of early stage endogenous vesicle that either arose from or embeddedin in the internal surface of exogenous vesicles were found The general character of the polycystic echinococcus collected from the gerbil in Inner Mongolia is similar to that of Echinococcus vogeli which is the pathogen of polycystic hydatid disease and has only been reported in Central America and South America,but is distinguished from it by the structure and morphology of its primary exogenous vesicle,the primary exogenous vesicle of larval Echinococcus vogeli Rausch et Bernstein,1972 has a large plaque from which have arisen the flattened primary vesicles,prior to onset of exogenous proliferation (Rausch and D'Alessandro,1999) Since the life history of this new metacestode is not known,it is tentatively named Polycystia neimonguensis sp nov国家自然科学基金重点项目 (3 973 0 40 0

SCANNING ELECTRON MICROSCOPICAL OBSERVATION ON ADULT NEOBENEDENIA MELLENI(MONOGENEA,CAPSALIDAE)

报道寄生于福建海水养殖鱼类高体的玫氏新本尼登虫 (单殖吸虫纲 ,分室科 )的扫描电镜观察。虫体体表无棘 ,前吸器和后吸器盘状。副甲片、前钩和后钩位于鞘内Neobenedenia melleni(MacCallum,1927) Yamaguti,1963,a capsalid monogenean parasitising the skin of marine cultured fish,Seriola dumerili(Carangidae) was studied by scanning electron microscope (SEM).The body surface of N.melleni lacks spines.Both the anterior attachment organs and haptor are disc shaped,the accessory sclerites,anterior hamuli and posterior hamuli are sheathed in tegument.Accessory sclerites possess sharp distal tips.Anterior hamuli and posterior hamuli possess curved tip,and marginal hooklets are arranged radially around haptor.ThisworkwassupportedbytheFujianProvincialNaturalScienceFoundation (B 991 0 0 0 5

Correlation between use of social software and high risk behaviors of HIV infection among MSM in Beijing

目的了解北京市男男性行为者（MSM）社交软件的使用与人类免疫缺陷病毒（HIV）感染及其影响因素的相关性,为今后干预工作提供依据。方法 2016年3—5月,由社区小组招募MSM进行问卷调查,同时进行HIV抗体检测。结果共招募215名MSM,回收有效问卷214份。214人的平均年龄为30.8岁;学历在大专及以上者占73.4%;同性恋者占72.4%;84.6%的人主要通过社交软件寻找性伴。214人中确证HIV感染5人,阳性率为2.3%。分析显示,是否使用社交软件、是否使用同性社交软件和是否使用普通人群社交软件均与HIV感染不相关。多因素分析结果显示,受教育程度高、性取向为同性恋的人更多地使用同性社交软件。63.1%被调查者自述曾经在社交软件上了解到艾滋病防治相关知识,但自述了解程度与是否通过社交软件获得相关信息不相关。结论 MSM社交软件的使用与HIV感染情况无关,也没有增加其对艾滋病知识的了解。但可以考虑改进同性社交软件功能,增加艾滋病防治知识内容,对MSM尤其是高学历、自我定位为同性恋的MSM进行艾滋病防治宣传。[Objective] To understand the correlation between the use of social software and human immunodeficiency virus（HIV）infection and its influencing factors among men who have sex with men（MSM）in Beijing,provide the basis for future intervention work.[Methods]From May to March in 2016,MSM was recruited by community groups to carry out a questionnaire survey and HIV antibody of them was detected.[Results]A total of 215 MSM were recruited and 214 valid questionnaires were recovered. The average age of the 214 MSM was 30.8 years old,73.4% had the education level in college and above,the homosexuals accounted for 72.4%,and84.6% of the respondents mainly found sexual partners through the social software. There were 5 cases of confirmed HIV infection among 214 MSM with the positive rate of 2.3%. The analysis revealed that whether the respondents use the social software,the homosexual social software and the general social software were not related to HIV infection. The results of multivariate analysis showed that people with higher educational level and homosexual orientation were more likely to use the homosexual social software. 63.1% of respondents reported that they had been obtained the knowledge of AIDS prevention and control from social software,but the understanding level was not related to whether the relevant information was obtained through social software or not.[Conclusion]The use of social software has no correlation with HIV infection among MSM,and does not increase their knowledge level of AIDS prevention and control. However,it is necessary to improve the function of the homosexual social software and increase the content of AIDS prevention and control knowledge to carry out the AIDS education among MSM,especially those with higher educational level and homosexual orientation

A New Genus Record of Phyllobothriidae from Marine Fish Dasyatis akajei in China

报道检获于厦门海域赤魟(Dasyatis akajei)肠道中的四叶目叶槽科蔷薇属叶枕蔷薇线虫Rhodobothriumpulvinatum Linton 1889,经鉴定比较,为我国鱼类绦虫属的新记录。本次共剖检赤魟46尾,阳性2尾,感染率为4.3％,感染强度为5～7条/尾,并对所获虫体进行了描述。Rhodobothrium pulvinatum, one species of Phyllobothriidae (Cestoda: Tetraphyllidea) from marine fish Dasyatis akajei in Xiamen, Fujian, China was reported. The genus Rhodobothrium Linton, 1889 was erected for R. pulvinatum from the intestine of Dasyatis kuhli. which was new genus record in China, Dasyatis akajei was also a new record of host. 46 of Dasyatis akajei were examined, 2 of which were infected with cestode, the infective rate was 4. 3%, the infective intensity of the cestode was 5-7, the description of the worm is provided in the present paper.厦门大学校级自选课题资助(2002

Effects of Shenling Baizhu Powder on expression of apoptosis related proteins of tumor chemotherapy model mice with H22 hepatocellular carcinoma cells

目的:研究参苓白术散(SLBZP)对H22肝癌移植瘤小鼠化疗后肿瘤凋亡相关因子Caspase -3、; Caspase-9、X染色体连锁凋亡抑制因子(XIAP)、血小板源生长因子-BB(PDGF-BB)、血管生成素-1(Ang-1)、血管生成素-2; (Ang-2)表达的影响。方法:SPF级昆明小鼠64只,将肝癌H22细胞悬液(2*10~7/mL)接种于小鼠右前腋皮下,1周全部形成移植瘤后,行; 环磷酰胺(CTX,0.20g/kg,0.2mL/10g)腹腔注射,建立肿瘤化疗模型。随机分组为8组:模型组,CTX(0.02g/kg)组,SLB; ZP高、中、低(6.00、3.00、1.50g/kg)剂量组,SLBZP高、中、低剂量+CTX(0.02g/kg)组,给药14d后,运用蛋白印迹; 法(Western blot)测定小鼠瘤组织中Caspase-3、; Caspase-9、XIAP蛋白表达;酶联免疫吸附(ELISA)测定小鼠血清PDGF-BB、Ang-1、Ang-2表达。结果:SLBZP联合治疗; 各组抑瘤作用明显,CT X组,SLBZP高剂量组,SLBZP高、中剂量+C T X组抑瘤率分别为52.39%、; 45.84%、58.41%、52.77%。与模型组比较,各治疗组血清PDGF-BB、Ang-1、Ang-2及瘤组织中XIAP表达呈现出被下调的趋; 势,瘤组织中Caspase-3、Caspase-9表达呈现出被上调的趋势,且以SLBZP高剂量加CTX组变化幅度最为显著(P<0.01,P<0.; 05)。结论:SLBZP联合化疗可以更有效调节H22肝癌移植瘤小鼠肿瘤凋亡相关因子的表达,促进肿瘤细胞凋亡可能是其改善化疗的机制之一。Objective: To investigate the effects of Shenling Baizhu Powder (SLBZP); on expression of apoptosis related proteins such as Caspase-3,; Caspase-9, X-linked inhibitor of apoptosis (XIAP), PDGF-BB, Ang-1, Ang-2; of tumor chemotherapy model mice with H22 hepatocellular carcinoma; cells. Methods: Sixty-four Kunming mice were subcutaneously injected; with suspension of H22 hepatocellular carcinoma cells (2*10~7/mL) into; the right anterior armpit. After 1 week, all transplanted tumors were; formed and the mice were received intraperitoneal injection with cytoxan; (CTX) with the dosage of 0.2g/kg to establish the tumor chemotherapy; model. Then mice were randomly divided into eight groups. CTX group was; treated with CTX (0.02g/kg), the model group was treated with; physiologic saline, three SLBZP groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg), and other three groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg) plus CTX (0.02g/kg). After all groups were treated for; 14 days, Western blot was used to detect the expression of Caspase-3,; Caspase-9 and XIAP proteins in tumor tissue, and protein expression of; PDGF-BB, Ang-1, Ang-2 in blood was measured by enzyme-linked; immunosorbent assay (ELISA). Results: The combined treatment group had; obvious effect on tumor inhibition. Tumor inhibitory ratios in CTX; alone, SLBZP (H), SLBZP (H) plus CTX, and SLBZP (M) plus CTX groups were; 52.39%, 45.84%, 58.41% and 52.77% respectively. Compared to the model; group, the protein expressions of PDGF-BB, Ang-1, Ang-2, and XIAP in; tumor tissue were obviously lower than those in all treated groups. And; the expressions of Caspase-3 and Caspase-9 in tumor tissue showed a; trend of up-regulation. And the effect in SLBZP (H) plus CTX group was; the most significant (P<0.01, P<0.05). Conclusion: SLBZP can more; effectively adjust the expression of apoptosis related proteins of tumor; in mice with H22 hepatocellular carcinoma cells, and promoting tumor; apoptosis may be one of the possible mechanisms of SLBZP to improve; chemotherapy in treating hepatic cancer.国家自然科学青年基金项目; 福建省自然科学基金项目; 福建省大学生创新创业训练项

包虫囊液外溢致过敏性休克抢救体会

目的探讨各种原因导致包虫囊肿破裂后囊液外溢致过敏性休克的诊断及抢救措施。方法回顾性分析33例各脏器包虫囊肿破裂后发生过敏性休克患者的临床资料、诊疗方法及效果。结果本组患者主要临床症状有:胸痛或腹痛、喉头水肿、呼吸困难、全身瘙痒、过敏性休克等;在立即通畅呼吸道、迅速建立静脉通道,使用血管活性药物升压、扩容,使用抗变态反应药物,手术清除过敏原后存活30例,死亡3例。结论选择快速实用的诊断方法确诊:如嗜酸粒细胞计数、包虫囊液皮内试验、补体结合试验和间接血凝试验及CT、B超,通畅呼吸道、抗过敏、扩容、升压,积极抢救及手术可提高抢救成功率

我国内蒙古大兴安岭北麓泡状肝包虫种类的研究 II.西伯利亚棘球绦虫(Echinococcus sibiricensis Rauschet Schiller,1954)

目的Rausch and Schiller(1954)在美国阿拉斯加白令海峡St.Lawrence岛从北极狐(Alopex lagopus)和雪撬狗(sledge dogs)发现西伯利亚棘球绦虫新种(Echinococcus sibiricensis sp.nov.),但诸多学者认为E.sibiricensis Rausch et Schiller,1954是欧洲的多房棘球绦虫(Echinococcus multilocularis Leuckart,1863)的地理株(或亚种)(Vogel,1957;Skrjabin and Abuladze,1964;Kumaratilake and Thompson,1982;Meyajaki,1991等)。于1985-2002年间,作者在我国内蒙古大兴安岭北麓草场多年进行泡状棘球蚴(alveolar Echinococcus)病原的野外调查和实验室鼠类的人工感染试验,结果发现该地区同地点存在有北美西伯利亚棘球绦虫、欧洲多房棘球绦虫及苏俄多房棘球绦虫三种不同"地理株",而且三种成虫常混合感染于同一终宿主沙狐(Vulpes corsac)的体内,它们的幼虫期也存在于当地的布氏田鼠(Microtus brandti)。2001年,作者再次检查当地沙狐151只,从中检出19只感染有泡状棘球绦虫成虫。含成熟虫体的13只阳性沙狐中,感染西伯利亚棘球绦虫有11只,其中2只单独感染此虫种,另9只还混合感染了欧洲多房棘球绦虫及苏俄多房棘球绦虫三虫种。在呼伦贝尔草原的泡状棘球蚴病原中,西伯利亚棘球绦虫是优势种,它在终宿主沙狐和中间宿主的感染率都高过其他虫种。本文着重介绍西伯利亚棘球绦虫的成虫子宫结构和它幼虫期在野外布氏田鼠及人工感染实验鼠体内的发育情况