高超声速升力体气动力气动热数值计算

升力体气动布局，由于其良好的高超声速气动特性、有效的内部空间和有益的防热特性，越来越受到气动工作者的重视，该文应用张涵信院士的NND格式思想，用数值模拟的方法对类似于美国X-33的升力体外形进行了气动力、气动热研究。特别是在粘性项的离散和表面热流率的计算中应用了散度和梯度的积分定义，避免了数值计算的奇异性，保证了通量守恒，提高了计算效率。结果表明气动热计算结果与美国同类飞行器结果接近，驻点热流率与经典理论结果一致

Medium Optimization for Antitumor Agent Mycoepoxydiene by Marine Lignicolous Fungi Diaporthe sp.

从海洋木栖真菌HLY-2分离得到的真菌环氧二烯(Mycoepoxydiene)是新发现的高抗肿瘤活性化合物。通过正交设计,改良普通半海水PD培养基的配方,比较固体和液体两种发酵方法,得到了使该化合物产量大幅提高的最佳培养条件:在固体发酵条件下土豆250g/L,海水300mL/L,葡萄糖30g/L,乳糖50g/L,磷酸二氢钾0·65mmol/L,硫酸铵1g/L,产量高达543mg/L,比普通的PD培养基提高了43倍,也比液体发酵的最高产量提高了近15倍。同时还对固体发酵和液体发酵造成的产量差异进行了分析。并检验了发酵产物的抗肿瘤活性。Mycoepoxydiene is a novel antitumor agent extracted from marine lignicolous fungi HLY-2, which is Diaporthe phaseolorum by molecule identification. The medium optimization for mycoepoxydiene by orthogonal design and the comparison of submerged fermentation and solid state fermentation were studied. The rusult is that the maximal yield of the compound is 543mg/L, which is 43 times compared to the customary half-seawater PD medium and 15 times to the best submerged condition. This optimum culture medium included potato 250g/L, seawater 300mL/L, glucose 30g/L, lactose 50g/L, KH_ 2 PO_ 4 0.65mmol/L and (NH_ 4 )_ 2 SO_ 4 1g/L in the solid state condition. Differentiation analysis between submerged and solid state fermentation, and antitumor activity of these ferment products were also studied. The antitumor activity of products of the optimum medium approached the pure compound.国家自然科学基金资助项目(No.2003AA624010);; 福建省科技项目基金(No.2004N003);; 福建省自然科学基金(No.C0210001

大孔吸附树脂分离纯化龙胆药材中龙胆苦苷和马钱子苷酸的研究

目的:建立利用大孔吸附树脂对龙胆药材中龙胆苦苷和马钱子苷酸进行富集和分离纯化的方法。方法:采用加速溶剂萃取法对龙胆药材中两种有效成分进行高效提取,比较了D301,AB-8,D101,XDA-1四种大孔树脂对龙胆苦苷和马钱子苷酸的吸附性能,最终确定采用D301型大孔对脂对二者进行富集吸附,对其工艺参数进行优化,全程采用高效液相色谱进行目标化合物浓度检测。结果:化优后的工艺参数为:上样浓度:0.2 g/mL,最大上样量:0.25 g龙胆药材/g树脂,最佳静态吸附时间:8 h,采用8%和55%的乙醇溶液对龙胆苦苷和马钱子苷酸分别进行洗脱;龙胆苦苷和马钱子苷酸分别富集在8%和55%的乙醇洗脱液中,洗脱液浓缩后冷冻干燥,可得到纯度分别为74.3%和80.9%的粗产物,龙胆苦苷和马钱子苷酸的回收率分别为70.11%和67.82%。结论:此法效率较高,操作简便,即可用于实验室制备少量的难以购置的标准品,也可进行放大研究,用于工业生产。国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 共建生物医药研发测试中心(LS-05-KJZX-76)资

Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints.The samples were extracted using ASE for 10 min under 100 ℃ and 9.65 MPa,and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively.Based on this method,the HPLC fingerprints of Radix gentianae were established.Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value,three compounds in water phase were identified as gentiopicroside,asafetida acid and loganic acid.There is no report of the compounds in chloroform phase.The content of gentiopicroside and loganic acid in samples of different groups were determined,separately.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90,however,the chloroform phase fingerprint congruence coefficients were within 0.62-0.99.This method can be used for determination of potent component in Radix gentianae and its quality control.Radix gentianae from different producing areas have the largest diversities,and the diversities embodied in the content of chloroform phase compounds.青岛“2004将才计划”(04-3-JJ-11);; 国家海洋局青年基金资助项目(2005602);; 崂山区政府区校共建生物医药研发测试中心资助(LS-05-KJZX-76)

Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode

目的建立胶束电动毛细管色谱(MEKC)和微乳液毛细管电动色谱(MEEKC)分析龙胆药材中龙胆苦苷和马钱子苷酸含量的方法。方法采用加速溶剂萃取法(ASE)对龙胆药材进行提取,萃取温度:100℃,压力:9.65MPa,萃取时间:10min。采用未涂层熔融石英毛细管(内径75μm,有效长度50cm)。分别考察了两种分离模式下电泳介质的构成和电泳过程中的各操作参数对样品分离过程的影响,优化了MEKC和MEEKC的分析条件,在各自对应的缓冲液体系下,MEKC和MEEKC分离电压分别为30和22kV,柱温均为25℃,检测波长均为238nm。结果在选定的工作条件下,龙胆苦苷和马钱子苷酸与其他组分达到了基线分离,两种成分的浓度与其响应信号值之间具有较好的线性相关性,加标回收率在96.3%～105.1%之间,检测限均低于10mg·L-1,对6处不同产地的龙胆药材进行了分析,并对测定结果进行了t检验,结果表明,两种模式下,测定结果之间不存在显著性差异,而不同产地的龙胆药材的龙胆苦苷和马钱子苷酸含量之间存在较大差异。结论本方法简便,准确,快速,重现性较好,可用于龙胆药材有效成分的含量测定和质量控制。OBJECTIVE To develop MEKC and MEEKC modes for the determination of gentiopicroside and loganic acid in extracts of Radix Gentianae. METHODS The analyte was extracted from Radix Gentianae samples by accelerated solvent extraction, and the extraction conditions were optimized. Separation and determination were carried out on a bared fused silica capillary(50 cm×75 μm) with corresponding buffer. The run voltage of MEKC and MEEKC were 30kV and 22 kV respectively. Detection wavelength of DAD was at 238 nm and column temperature was 25 ℃. RESULTS The developed ASE-HPCE method was simple and reliable for the determination of gentiopicroside and loganic acid in Radix Gentianae samples with a broad linear dynamic range, a recovery range of 96.3%～105.16%,and the detection limit was below 10 mg·L-1. The contents of gentiopicroside and loganic acid in six samples from different regions were determined by the developed method in two modes. T-test value indicated that the determined contents of gentiopicroside and loganic acid by MEKC and MEEKC were consistent. CONCLUSION The method is simple,accurate,rapid and with good reproducibility.It can be used to determine active components in Radix Gentianae.国家自然科学基金重点项目(20235020);; 青岛“2004将才计划”(04-3-JJ-11);; 崂山区校区共建生物医药研发测试中心资助(LS-05-KJZX-76

基因编辑技术在莱茵衣藻中的应用进展

基因编辑技术已经成为功能基因组学和作物分子育种精准且有力的工具。莱茵衣藻(Chlamydomonas reinhardtii,简称衣藻)是光合作用、黑暗异养代谢、厌氧代谢和生物制氢、营养和能量代谢等研究领域的重要模式生物。近20年来,基因编辑技术,如锌指核酸酶(ZFNs)、转录激活物样效应物核酸酶(TALENs)、CRISPR/Cas9和CRISPR/Cpf1等的发展,更加推动了以衣藻为模式生物的研究。现对衣藻中的核基因组靶向基因编辑技术的应用及最新研究进展等进行总结,以期为衣藻相关领域的研究提供参考

Review on the Effect of Bilingual Experience to Access Performance during Speech Production

本文介绍了双语经验对言语产生过程中通达能力的影响，言语产生是指个体通过口头言语表达想法、感受，而通达过程是言语产生的关键过程。基于语言系统的层次性，本文从亚词汇、词汇、短语和句子四个语言水平归纳了双语经验对通达能力造成消极影响的证据。基于此，从跨语言干扰、语言使用频率和词汇量三个角度梳理相关解释。未来研究需要关注双语经验对言语加工中不同方面的影响，不同双语经验对通达能力的影响，并整合相关解释。</p

Case Study on Quantitive Assessment of Demonstrative Industrial Project Model for Circular Economy

依据循环经济项目的定量评估系统,选取13项公共指标及2项选评指标,对厦门市狐尾山污水林地生态处理工程项目进行示范性评估.根据循环经济的特点,该项目属于减排型和循环再利用型.即能达到污水零排放和污水100%的循环再利用,削减进入厦门海域的污水排放量,有利于裸露山体绿化改造,还能产生一定的经济效益.对所选择的指标进行打分和评估,并使用加权平均法,计算狐尾山污水处理生态工程循环经济综合评价值,该工程的综合评价值为89.6分,表明项目具有较高的循环经济示范性,发展潜力大.Based on the quantitive assessment system of demonstrative industrial project model for circular economy.Thirteen common and two other indices were selected to assess a project of sewage disposal on mountain woodland at Huwei Mountain in Xiamen.The project can be characterized into pollution reduction and cyclic uses of resource and can increase the green cover,improve the mountain environment, and 100% reuse the domestic sewage without any discharges into coastal sea.The project were scored and assessed through a method of experts′ scoring and summarized using weighted average method for all indices of the Huwei Mountain sewage disposal project.The comprehensive score of the project is 89.6,and the result indicated that the project have relative high potential for demonstration.国家自然科学基金资助项目(40671116);; 福建省自然科学基金计划资助项目(D0720002);; 教育部新世纪优秀人才支持计划项目(教技司[2007]209号);; 福建省高校新世纪优秀人才支持计划项目(闽教科[2006]35号

胫骨前肌训练对脑卒中偏瘫患者患肢功能和步态的影响

目的:探讨早期胫骨前肌训练对急性脑卒中偏瘫患者患肢功能和偏瘫步态恢复的影响。方法:将92 例急性脑卒中伴肢体功能障碍的患者随机分为实验组(46)和对照组(46),两组病人在生命体征稳定,神经 病学体征不再进展48小时后开始接受神经科常规治疗和康复训练,实验组在常规治疗和康复训练的同时 进行胫骨前肌的训练。结果:训练二周后,实验组的肌力、踝关节活动度、步行速度和步幅的评分与对照组 比较差异有统计学意义(p&amp;lt;0.05)。结论:在常规早期康复治疗的基础上加胫骨前肌的训练能更有效的改 善脑卒中偏瘫患者患肢功能和步态的恢复。</jats:p