我国地方税系存在的问题及对策

本文分析了我国地方税系存在的问题,并提出了改革和完善我国地方税系的对策,以期为构建符合我国实际的、规范化的地方税收体系提供参考

和谐社会目标下理想收入分配格局初探

一、理想社会收入分配格局研究现代社会的发展表明,中等收入阶层越大,对社会的和谐、稳定、健康发展越有利。中等收入是指社会成员收入处于中等水平。笔者认为,中等收入水平是个相对数值,是针对整个社会的总体收入水平而言的。中等收入水平应该是一个区间

分子内电荷转移荧光探针法测定表面活性剂的临界胶束浓度

研究了分子内电荷转移荧光体4-二甲氨基查尔酮(DMAC)在表面活性剂水溶液中的荧光光谱。观察到随表面活性剂浓度增大,DMAC 的荧光光谱先红移然后蓝移,而荧光强度呈“S”型变化并于光谱位移方向转折处突跃。突跃处对应的表面活性剂浓度即为其临界胶束浓度(cmc)。结果表明由该法测定的 cmc 与已报道值符合,说明方法的可行性与准确性。用该法测定表面活性剂/β-环糊精混合体系的 cmc,表明β-环糊精的存在诱导了胶束的形成

Biological mechanism underlying the formation of greasy fur

腻苔是一种临床常见的病理性舌苔,主湿浊、痰饮、食积等,在中医临床诊断与治疗中有重要的临床意义与辨证价值,研究腻苔形成的现代生物学机制有重要的理论; 和实践意义。文章从舌表面脱落细胞学、微生态学、相关蛋白与基因表达及物质代谢等方面对腻苔形成的生物学机制进行了综述,并对腻苔与肠道微生态的关系,腻; 苔与现代医学疾病的关系进行了阐述,以期从现代生物医学角度对腻苔形成的物质基础、发生机制、变化规律及与相关疾病的关系作相关阐明,为疾病的中医诊断与; 治疗提供借鉴和帮助。Greasy fur is a common pathological tongue coating and is mainly seen in; the clinical practice in traditional Chinese medicine (TCM), such as in; the dampness turbidity, the phlegm and fluid retention, the dyspepsia or; food accumulation, and so on. Greasy fur shows important clinical; significance and differentiation value in TCM diagnosis and treatment,; there is also great theoretical and practical significance in the; studying of the modern biomedical mechanism underlying the formation of; the greasy fur. Here we summarized the formation mechanism of the greasy; fur from the aspects of the shedding cytology of the tongue surface, the; microecology, the expression of the relative genes and proteins and the; metabolism. The relationship between the greasy fur and the intestinal; microecology, and between the greasy fur and the diseases were also; discussed in this paper. Through summarizing these related research; literatures, we want to elucidate the material basis, the occurrence; mechanism and the changing rules of the greasy fur from the perspective; of the modern biomedicine, and to elucidate its relationship with some; related modern medicine diseases, thus to provide references and; assistance for the disease diagnosis and treatment in TCM clinic.国家重点研发计划重点专项; 教育部新世纪优秀人才支持计划; 国家自然科学基金项

抗CD5单链抗体的基因构建、蛋白纯化及活性鉴定

目的构建高表达的抗CD5单链抗体(scFv anti-CD5)工程菌,为开展以抗CD5单链抗体为构件的肿瘤免疫治疗研究奠定基础。方法从Genbank中获得抗CD5单克隆抗体H65的重链可变区(VH)和轻链可变区(VL)的基因序列,用连接肽的编码序列连接,用重叠PCR技术获得目的基因,酶切后插入pET22b(+)构成重组质粒。测序正确的重组质粒转入E.coliBL21(DE3)诱导表达,产物经Ni-NTA柱纯化后复性,流式细胞术和Western blot-ting检测活性。结果获得序列正确的重组质粒,工程菌表达量高,产物复性后具有识别CD5抗原的活性。结论成功构建了高表达scFv anti-CD5的工程菌,为scFv anti-CD5的应用奠定了基础

滑轮对绳拉力损耗影响的分析研究

针对滑轮—绳索系统处于平衡和非平衡两种情况,从理论上分析并计算出滑轮两端的绳索拉力,研究滑轮对绳索拉力损耗的影响规律。以绳牵引机器人的动平台做单自由度振荡为例,采用MATlAb软件计算出动平台在不同振幅、不同振动频率的情况时,牵引动平台运动的绳索在滑轮两端的拉力差值,并分析在滑轮后测量绳索拉力以预测滑轮前绳索拉力的误差程度。文章所做研究为预测滑轮对绳索拉力的影响,以及滑轮后测量绳索拉力带来的误差提供了参考。国家自然科学基金项目(11472234

松本駅前商業空間における商業機能の変容 : 公園通りを事例に

departmental bulletin pape

去氧胆酸钠水溶液中4－二甲氨基查尔酮荧光的超常增强

去氧胆酸钠水溶液中４－二甲氨基查尔酮荧光的超常增强江云宝，王秀娟，林莉，黄贤智，许金钩（厦门大学化学系，现代分析化学研究所，厦门３６１００５）关键词双自由基激发态，荧光探针，胆酸型甾族表面活性剂，缔合类似于电子给体受体取代的芭类化合物，４－二甲基氨基..

去氧胆酸钠水溶液中4－二甲氨基查尔酮荧光的超常增强

去氧胆酸钠水溶液中４－二甲氨基查尔酮荧光的超常增强江云宝，王秀娟，林莉，黄贤智，许金钩（厦门大学化学系，现代分析化学研究所，厦门３６１００５）关键词双自由基激发态，荧光探针，胆酸型甾族表面活性剂，缔合类似于电子给体受体取代的芭类化合物，４－二甲基氨基..

Polymorphism of Fatty Acid of Ralstonia solanacearum in Fujian Province

【目的】利用气相色谱技术检测福建省的40株青枯雷尔氏菌(Ralstonia solanacearum)菌株细胞内的脂肪酸,分析其脂肪酸分布的多态性;研究青枯雷尔氏菌脂肪酸多态性与青枯雷尔氏菌现有种下分化方法之间的关系。【方法】对40株青枯雷尔氏菌脂肪酸进行气相色谱分析,比较同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌脂肪酸的分布;对40株青枯雷尔氏菌脂肪酸进行聚类分析,分析聚成的各类青枯雷尔氏菌脂肪酸的特点以及脂肪酸多态性与其生理小种、生化型和致病性之间的关系。【结果】同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌,其脂肪酸都存在着明显的多态性;对40株青枯雷尔氏菌的脂肪酸进行聚类分析,可以聚成3类,即groupⅠ、groupⅡ和groupⅢ;青枯雷尔氏菌生理小种1存在着不同的脂肪酸类群,青枯雷尔氏菌脂肪酸多态性与其生化型之间不存在相关性,但是脂肪酸和致病性之间存在一定的相关性:groupⅠ为无致病性菌株,groupⅡ为过渡性菌株,groupⅢ为强致病性菌株。【结论】福建省青枯雷尔氏菌脂肪酸分布存在着明显的多态性;青枯雷尔氏菌脂肪酸多态性与致病性之间存在一定的相关性,脂肪酸有望成为青枯雷尔氏菌小种鉴定的新指标。【Objective】The fatty acids of 40 strains of Ralstonia solanacearum isolated from different hosts in the fields in Fujian Province were detected by gas chromatography (GC). The polymorphism of R. solanacearum fatty acids relating to the pathogenicity was observed. 【Method】 The MIDI system and cluster analysis were introduced in analyzing fatty acids to display the relations among the polymorphism, race, biovartype and pathogenicity. 【Result】 The results showed that the patterns of fatty acids were significant different in R. solanacearum strains both isolated from the different hosts and the different body parts of the same hosts. According to the fatty acids the strains were clustered into three groups, e.g. group Ⅰ relating to the strains with non-pathogenicity, group Ⅱ in which the strain pathogenicity was changeable with some virulent and avirulent ones, and group Ⅲ respondent to high pathogenicity. It was proved that the model of fatty acids has no relations to races and biovartypes in R. solanacearum. 【Conclusion】It is the fist time to describe the polymorphism of fatty acids in R. solanacearum in this paper. The pathogenicity could be grouped by the models of fatty acids to distinguish the pathogenicity, which could be used in the identification of R. solanacearum under species differentiation.国家“863”项目(2002AA244031);; 福建省青年科技人才创新项目(2003.J046