Response of phytoplankton to nutrients addition in the upwelling regions of the Taiwan Strait

2006年6月在台湾海峡近岸上升流区通过表层水体营养盐添加的现场培养实验,研究该海区营养盐限制情况及其浮游植物水华产生的主要影响因素。对营养盐,叶绿素a浓度和浮游植物细胞丰度进行了测定,结果表明,实验中不存在明显的硅限制;氮磷营养盐均存在明显的限制,且氮限制情况更为严重。营养盐添加后,冰河拟星杆藻(Asterionellopsis glacialis)等硅藻迅速生长成为优势藻种,其对氮磷的利用机制有所不同。对氮营养盐采取吸收后迅速同化利用,相较于硝酸盐的补充,氨氮补充条件下优势硅藻更易迅速生长并迅速死亡;对磷营养盐的利用则由于体内磷库的存在,采用迅速吸收后贮存在体内慢慢消耗的利用机制。氮营养盐的补充是上升流期间浮游植物水华产生的主要因素。 【英文摘要】 In situ incubation experiments of nutrients addition to surface water from the upwelling region in the Taiwan Strait were conducted to investigate nutrient limitation of this area and the main factors that caused the phytoplankton bloom in June,2006.Variation of nutrients,Chl-a concentration and phytoplankton species density were analyzed.The results showed no clear limitation by silicon,however clear nitrogen and phosphate limitations were found,and nitrogen limitation was more serious than phosphate limit...国家自然科学重点基金资助项目(40331004)~

pJAK2 polypeptide,an antagonist of suppressors of cytokine signaling-1,can enhance the antitumor effect of dendritic cells

目的:探讨细胞因子信号转导抑制因子-1(SuPPrESSOrS Of CyTOkInE SIgnAlIng1,SOCS1)拮抗物PJAk2多肽(氨基酸序列号为1001-1013)参与树突状细胞(dEndrITIC CEllS,dCS)的体外诱导培养后对dCS抗肿瘤作用的影响。方法:采集健康人外周血,离心获得单个核细胞,用重组人粒细胞巨噬细胞集落刺激因子(rECOMbInAnT HuMAn grAnulOCyTE-MACrOPHAgE COlOny STIMulATIng fACTOr,rHgM-CSf)及重组人白细胞介素-4(rECOMbInAnT HuMAn InTErlEukIn-4,rHIl-4)诱导dCS,第5天分为4组:单纯dCS培养(对照)组、抗原负载(lySATE-dCS)组、多肽修饰(PJAk2-dCS)组和抗原与多肽共培养(lySATE+PJAk2-dCS)组,第6天各组加入肿瘤坏死因子-α(TuMOr nECrOSIS fACTOr-AlPHA,Tnf-α)促成熟。倒置显微镜下观察dCS形态;fCM法检测dCS表型;乳酸脱氢酶(lACTATE dEHydrOgEnASE,ldH)细胞毒实验检测各组细胞毒性T淋巴细胞(CyTOTOXIC T lyMPHOCyTE,CTl)对胃癌细胞bgC-823的靶向杀伤作用;ElISA法检测白细胞介素-12(InTErlEukIn-12,Il-12)和γ干扰素(InTErfErOn-γ,Ifn-γ)的水平。结果:与未加入诱导剂组相比,各组均成功诱导出成熟dCS,均高表达Cd80、Cd83、Cd86和人类白细胞dr抗原(HuMAn lEukOCyTE AnTIgEn dr,HlA-dr),但以lySATE+PJAk2-dCS组的表达水平最高。在10:1~30:1的效靶比范围内,CTl杀伤作用与效靶比呈正相关。当效靶比为30:1时,对照组的CTl杀伤率达(19.77±2.34)%,低于其他3组(P<0.01),而lySATE+PJAk2-dCS组较lySATE-dCS组及PJAk2-dCS组都高(P<0.05)。lySATE+PJAk2-dCS组培养上清液中Il-12及Ifn-γ的分泌水平明显高于对照组(P<0.01)。结论:SOCS1拮抗物PJAk2多肽(1001-1013)可增强dCS对胃癌细胞的抗原递呈及特异性抗肿瘤作用。Objective:To investigate the effect of pJAK2 polypeptide,an antagonist of SOCS1(suppressors of cytokine signaling 1),on antitumor effect of in vitro cultivation-induced DCs(dendritic cells).Methods:Peripheral blood was collected from the healthy volunteers,and the PBMCs(peripheral blood mononuclear cells)were isolated.DCs were induced by rhGM-CSF(recombinant human granulocyte-macrophage colony-stimulating factor)and rhIL-4(recombinant human interleukin-4).On the fifth day,DCs were divided into four groups:control group,Lysate-DCs group,pJAK2-DCs group,and Lysate + pJAK2 DCs group.On the sixth day,TNF-α(tumor necrosis factor-alpha)was added into each group.The morphological features of DCs were observed under an inverted microscope;the phenotypes were detected by FCM(flow cytometry);the killing effect of CTLs(cytotoxic T lymphocytes)on gastric cancer BGC-823 cells was evaluated by LDH(lactate dehydrogenase)cytotoxicity test;the concentrations of IL-12(interleukin-12)and IFN-γ(interferon-γ)were detected by ELISA(enzyme-linked immuno sorbent assay).Results:Mature DCs presented typically morphological and phenotypic features;the DCs in Lysate + pJAK2-DCs group had the highest expression levels of CD80,CD83,CD86 and HLA-DR(human leukocyte antigen DR).When the ratio of effectors to target cells ranged from 10:1 to 30:1,the killing activity of CTLs had a positive correlation with the ratio.When the ratio of effectors to target cells was 30:1,the killing activity of CTLs in the control group was(19.77±2.34)%,which was lowest as compared with the other groups(P < 0.01),meanwhile the killing activity of CTLs in Lysate + pJAK2-DCs group was higher than those in Lysate-DCs and pJAK2-DCs groups(P < 0.05).The levels of IL-12 and IFN-γ secretion in Lysate + pJAK2-DCs group were apparently higher than those in the control group(P < 0.01).Conclusion:An antagonist of SOCS1,pJAK2 polypeptide,can enhance the ability of antigen presentation and specific antitumor effect of DCs on gastric cancer cells.南京军区医学科技创新课题资助项目(编号:10MA068); 福建省自然科学基金面上项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014

暹罗鳄多肽粉提高机体免疫力及抗疲劳的效应

为研究暹罗鳄多肽粉对小鼠免疫力及抗疲劳效应的影响,对小鼠的免疫能力和相关血清指标进行了测定,同时也对小鼠的运动能力及疲劳代谢指标进行了测定.实验结果显示:暹罗鳄多肽粉可以促进小鼠胸腺和脾脏的生长,免疫力得到提高.相对于对照组小鼠,暹罗鳄多肽粉组小鼠血清中的总蛋白、血钙、白蛋白含量升高(p<0.05),谷丙转氨酶(ALT)的活力和血糖浓度下降(p<0.05).小鼠爬绳及负重游泳实验发现服用了暹罗鳄多肽粉的小鼠垂直爬绳时间和负重游泳时间均高于对照组的(p<0.01),小鼠的耐力增强.进一步通过建立抗疲劳小鼠力竭游泳模型研究了暹罗鳄多肽粉的抗疲劳动物学功效,结果显示相对于对照组,暹罗鳄多肽粉组小鼠血液中尿素氮(BUN)和乳酸(LA)的含量以及乳酸脱氢酶(LDH)的酶活力均降低(p<0.05),而超氧化物歧化酶(SOD)的酶活力升高(p<0.05).综上结果表明暹罗鳄多肽粉可能有保肝护肝,提高肌体免疫力以及抗疲劳等功效.福建省自然科学基金项目(2016J05105);;广东省自然科学基金博士启动项目(2016A030310374);;厦门大学生命科学学院-福建鼍龙鳄鱼生物制品研发协同创新中心项目(XDHT2017004C

The EFFect of Preparation Conditions on the Properties of SnO_2/Si

研究了沉积温度、SnCl4溶液的浓度、掺Pd等对SnO2/SI光电压的影响，测量了SnO2/SI的光电压谱，得出最佳的制备条件，进行了有关计算和分析。Some tin oxide Films are deposited by CVD method on single crystal silicons.The eFFect of diFFerent deposition conditions, such as diFFerent substrate temperatures, SnCI4 solutions and Pd doping,on photovoltaic properties of the Films are studied.The best preparation conditions are obtained.Relative parameters is calculated From the photovoltaic spectra.国家自然科学基金;福建省自然科学基

Estimation of the sources of organophosphorus pesticides in Xiamen sea area,

利用SPEGCFPD对厦门附近海域的微表层、表层、底层海水及九龙江入海口高、低潮水中有机磷农药进行了分析.结果表明,有机磷农药的含量范围为:低于检测限～725.54ng·L-1,平均值为136.47ng·L-1.研究海区中马銮内湾有机磷农药污染最为严重(165.77～453.42ng·L-1,平均296.01ng·L-1),九龙江口、西海域和西南海域污染水平接近(16.26～200.65ng·L-1,平均84.59ng·L-1),东部海域有机磷农药污染最轻(10.38～66.01ng·L-1,平均38.14ng·L-1).九龙江入海口高潮水中农药的浓度明显低于低潮水中浓度,说明海域中多数农药污染来源于九龙江流域输入,少数农药来源于海域周边地区或九龙江流域和海域周边地区共同输入,微表层对有机磷农药的富集作用不明显. 【英文摘要】 Organophosphorus Pesticides (OPPs) were determined in microsurface water, subsurface water and bottom water in Xiamen sea area with SPE-GC-FPD. The result showed that the levels of all OPPs ranged from below detection limit to 725.54ng·L -1, the mean was 136.47ng·L -1. The pollution in Maluan Bay was the most serious(165.77～453.42ng·L -1,mean 296.01ng·L -1) and the Eastern Sea was the slightest(10.38～66.01ng·L -1,mean 38.14ng·L -1). Meanwhile, the levels of OPPs in Jiulong estuary were closed to that in the...国家自然科学基金(No.40106012)~

Preliminary estimate for the contaminations fluxes from Jiulong River to the sea

河流污染物入海通量的研究是研究海水污染的内容之一。本文对目前常用的河流污染物通量估算方法进行了分析,根据九龙江的水文水质监测数据,选择高锰酸钾指数与NH3-N作为代表性水质监测项目,利用各种估算公式进行污染物入海通量估算,对估算结果进行比较发现部分公式适用性较好。估算结果表明,2003年九龙江污染物入海通量CODMn大约为21 000 t/a,NH3-N大约为2 500 t/a。根据与其他研究结果的对比,探讨了河流污染物入海通量估算的特点,对不足之处作了说明。 【英文摘要】 Study of the contaminations fluxes from river to the sea is part of the research of marine pollution.The popular calculation formulas of contaminations fluxes in river were analyzed.According to the flow and water quality monitoring data in Jiulong River,COD_(Mn) and NH_3N fluxes were calculated by the different formulas.The calculation results show that some formulas are effective.In 2003,the COD_(Mn) fluxes from Jiulong river to the sea was about 21 000 t/a,and the NH_3-N fluxes was about 2 500 t/a.Compar...福建省青年科技人才创新项目(2005J065

钛酸纳米管前驱体水热制备TiO_2:水热媒介对结晶度及光催化活性的影响

以钛酸纳米管为先驱体,180℃24 h同一水热条件下,利用H2O、HNO3、KBF4以及HNO3+KBF4的4种水热媒介溶液,制备了高结晶度高光催化活性的TiO2,并研究了水热媒介对TiO2结晶度、表面羟基和光催化活性的影响。通过扫描电镜(SEM)、X射线衍射(XRD)、红外测试(FTIR)、X射线光电子能谱(XPS)、紫外可见漫反射吸收光谱(UV-Vis)进行了结构表征。光催化活性通过降解甲基橙溶液进行了评价。结果表明,与其他3种媒介相比,HNO3+KBF4溶液为水热媒介制备的锐钛矿晶型TiO2,结晶度高,表面羟基含量高,因此,呈现高的光催化活性

协作征管:共建环保“税时代”

背景材料:1月1日起,《中华人民共和国环境保护税法》《中华人民共和国环境保护税法实施条例》同步施行。环保税的开征,意味着我国税收在生态环境方面的调控作用进一步强化,推动企业加速绿色转型