中国の名門大学における外国人学生向け中国語教育

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长喙毛茛泽泻的花器官发生

用扫描电镜对长喙毛茛泽泻（ＲａｎａｌｉｓｍａｒｏｓｔｒａｔｕｍＳｔａｐｆ．）的花器官发生进行了观察。该种的花器官发生在泽泻科中既典型又比较特殊。其特殊之处在于从花原基的发生直至最外轮６枚雄蕊原基的发生过程都存在单向发育：３个萼片原基和３个花瓣原基的发生存在次序性，萼片原基和花瓣原基均为顺时针发生，邻近花序侧生分支复合芽的萼片原基和花瓣原基倾向于优先发生；最外轮６枚雄蕊的发生是成对的按对萼的形式发生。这是以往对该属花器官发生研究没有注意到的。由雄蕊原基向心皮原基的转变发生在与最外轮６枚雄蕊相交替的６个位点

太子参环肽类化合物Pseudostellarin E对3T3-L1前脂肪细胞分化和葡萄糖吸收的作用

目的探讨太子参环肽类化合物Pseudostellarin E是否具有治疗2型糖尿病的潜力。方法在3T3-L1前脂肪细胞中应用了葡萄糖吸收试验和分化模型。结果 Pseudostellarin E不仅加速3T3-L1前脂肪细胞分化进程,而且在高浓度(30m M)葡萄糖条件下增加了分化的脂肪细胞对胰岛素刺激的葡萄糖的吸收。结论上述结果表明,Pseudostellarin E可能具有治疗糖尿病的潜能。国家自然科学基金面上项目(81773601);;福建省教育厅2015年第二批高等学校优秀学科(专业)带头人赴海外访学研修资助项目(闽教人[2015]103号);;福建省自然科学基金计划资助项目(2015J01065);;福建省卫生系统中青年骨干人才培养项目2015年资助计划基础项目(2015-ZQN-JC-45);;福建省厦门市科技局科技计划高校创新项目(3502Z20143026);;厦门医学院科研项目(Z2013-12,Z2013-25

人红细胞凝集型单抗的制备与鉴定

目的制备人红细胞凝集型单克隆抗体并进行鉴定。方法以3种方式处理人血红细胞,获得红细胞免疫原,分别免疫Balb/c小鼠。应用杂交瘤技术,取免疫小鼠的脾细胞与Sp2/0骨髓瘤细胞融合,以凝集实验筛选分泌凝集型抗体的杂交瘤细胞,继而用有限稀释法克隆3次,并对杂交瘤细胞的稳定性进行测定。通过腹水法制备抗体,亲和层析法纯化抗体,对抗体的特性进行研究。结果 3种方式获得的免疫原均能建立有效的免疫反应,低渗加超声破碎法所获得的小鼠血清抗体效价最高。通过融合获得8株可稳定分泌抗体的杂交瘤细胞,其中,4A1分泌的抗体凝集效价最高。抗体可使4种人血型的红细胞凝集,无种属交叉凝集反应。结论有效制备了针对人红细胞的凝集性单抗,为其之后的应用研究奠定了基础。福建省省属公益类项目(2015R1036-3,2016R1034-2,2017R1036-3);;福建省科技计划(2014Y0082,2014Y0083

紫菜降血压肽大鼠体内降压效果研究

目的观察紫菜降血压肽对高血压大鼠(SHR)和京都Wistar大鼠(WKY)的降压效果,并与降压药物卡托普利的作用效果比较。方法分别以500、1 000和1 500 mg.kg-1剂量的紫菜降血压肽一次性给药后,每隔2 h测量大鼠收缩压(SBP),连续测量8 h;长期实验持续4 w,每天1 500 mg.kg-1灌胃一次,每隔1周测量一次SBP、心率及体重。结果 SHR给予紫菜降血压肽后,血压均显著下降;而正常血压大鼠WKY服用紫菜降血压肽后血压没有显著变化,服用卡托普利血压仍保持显著下降的趋势。结论紫菜降血压肽对SHR具有显著的降压效果,长期服用可稳定血压,对正常血压大鼠无明显影响;卡托普利对SHR的降压效果虽高于紫菜降血压肽,但会导致正常大鼠血压过低

都市部の高所花粉計測法の開発および降水によるアレルゲンの溶出挙動

In this study, we have collected Japanese cedar pollen from the roadside of two urban residential zones and a high place (about 35 m) of Saitama City during the pollination period in 2009. For the purpose of analyzing the airborne cedar pollen vertical distribution, I observed it both at roadside and 35m high place during the cedar pollen airborne period. Then, Japanese cedar pollen allergen was increased at sunny days after rainfall in atmosphere. We thought that there are more few pollen counts of the high altitude than road edge, but, the experiment result is showed that the pollen count of the high altitude is different from road edge for two days, and more than roadside. High affected possibility is thought about by this experiment result. Therefore, it is supposed that crustal particles (Kosa) and rainfall may be increased eluted rate of allergen contents and lead to release and transit them to fine particles. Fine particles were more suspended than coarse particle (such as pollen). Therefore, It will be thought that we need the vertical distribution investigation not only pollen counts but also allergen concentrations according to the altitude in future.textapplication/pdfdepartmental bulletin pape

上海动物园圈养梅花鹿（Cervus nippon）丰容效果的行为学评价

2013年14月期间,对上海动物园圈养梅花鹿进行了食物丰容,并基于动物行为对丰容效果进行了初步评价。采用瞬时扫描取样法观察记录14只梅花鹿丰容前后行为在时间和空间上的改变。研究表明,环境丰容对梅花鹿行为在时间和空间分配上均存在显著影响（P<0.05）。从行为类型来看,丰容前后梅花鹿的摄食行为、反刍行为、乞食行为和运动行为存在显著差异（P<0.05）。丰容使梅花鹿的摄食行为频率明显增加,从而使休息和聚群等其他各类行为频率减少。本研究对空间区域划分的手段能够为圈养区建设提供科学建议,对改善梅花鹿圈养条件具有积极的作用和效果

Enantioseparation of 1-substituted 3-arylseleno-2-propanols onPolysaccharide Tris(3,5-dimethylphenylcarbamate)Chiral Stationary Phases

采用Chiralcel OD H和Chiralpak AD H手性柱,研究5种新近合成的外消旋双芳基硒代甘油硫醚和 4 种芳基硒代甘油烷氧醚对映体的高效液相色谱拆分方法.对比这两种手性固定相、异丙醇和乙醇流动相调节剂、以及样品结构对硒代甘油醚色谱拆分的影响.除样品(1c)外,其他8个外消旋硒代甘油醚样品均可达到基线拆分.The enantiomer separation of nine pairs of racemic 1-arylthio-3-arylseleno -2-propanols and 1-alkoxy-3-arylseleno-2-propanols was achieved with high performance liquid chromatography by using cellulose tris(3,5-dimethylphenyl carbamate) (Chiralcel OD-H) and amylose tris(3,5-dimethylphenylcarbamate) (Chiralpak AD-H) as chiral stationary phases and hexane-2-propanol or hexane-ethanol as mobile phases in various percentages.Amylose tris(3,5-dimethylphenylcarbamate) exhibited higher chiral recognition ability compared to cellulose tris(3,5-dimethylphenylcarbamate).The effects of alcohol in the mobile phase and solute structures on the retention and resolution were investigated.On the Chiralcel OD-H column,solutes elute faster with ethanol than with 2-propanol modifier as expected from the higher polarity of ethanol. However,on Chiralpak AD-H column the retention factors of all the enatiomers increase,the enantioseparation factors and resolutions dramatically change on changing 2-propanol to ethanol.The explanation may be that ethanol changes the higher order structure of amylose.The enantioseparation on the Chiralpak AD-H column is much more influenced by the kind of alcohol in the mobile phase than the Chiralcel OD-H.Under optimized conditions,baseline enantioseparation of eight pairs of racemic 1-substituted 3-arylseleno-2-propanols were obtained