The Research of Chen Moxiang's Chinese Opera Study

陈墨香是民国时期著名的京剧票友、编剧家以及戏曲批评家。他的戏曲理论批评和研究注重从艺术本体出发，对京剧进行整体性的研究和论述，并提出一些颇具创建性的主张和观点。他注意到京剧艺术厚重的民间文化底蕴，有意识地对京剧表演与民间习俗的互动关系进行考察，拓宽了京剧学的研究路径。陈墨香的京剧研究以丰赡的戏曲活动经验为支撑，虽然经验描述多于理论阐发，未能形成严整的理论体系，但他的京剧批评和研究，对于二十世纪的戏曲理论批评乃至今天的京剧研究都具有重要的参考价值和意义。 本文以陈墨香的京剧学研究著述为对象，在对原始资料进行归纳、总结的基础上，结合前人的研究成果，对陈墨香的京剧学研究进行深入的分析和研究，以期全...Chen Moxiang was famous as an amateur performer of Peking Opera, scenarist and drama critic in the period of Republic of China. Based on the art noumenon, Chen Moxiang studied the completeness of Peking Opera, and raised some constructive views. He noticed that with profound folk cultural heritage, it was significant to consciously study the interaction between Peking Opera and folk customs, which...学位：艺术学硕士院系专业：人文学院_戏剧与影视学学号：1022013115243

吊诡意义:略论《逃亡》的寓言图景

高行健的戏剧富有寓言性质,这在《逃亡》中同样也有体现。语义混乱、人物陷于玄谈、剧情人物叙事中止而依托难以逆料的处境变化,这些形式的应用使得《逃亡》最终消解了彼岸与此岸的联系,意义如“羚羊挂角,无迹可求“。高行健以戏剧为“艺乘“,将自我生命的体验进行直觉观照,因此他的戏剧呈现出一派荒原景象

笑与泪:来自新编梨园戏《陈仲子》的黑色幽默

新编梨园戏《陈仲子》是一部颇具黑色幽默的剧作。一方面,陈仲子为求绝对的贞廉,不断避离世俗,甚至连最基本的生存物质也加以否定,充满荒诞意味;另一方面,陈仲子的求索表达的是民族的一种精神追求在时代中的变奏,闪烁着哲思的光芒。此外,剧中"门子"的插科打诨与"歌队"的设置,也在不断消解舞台与现实的阈限,完成对现实的指涉。《陈仲子》一剧,笑中含泪,引人深思

生脉注射液致急性变态反应1例

患者,男,71岁,既往无药物过敏史。因左上肺癌并纵隔淋巴结肿大,胸骨、肋骨多发转移入院,行第2周期gP联合重组人血管内皮抑制素化学治疗。治疗第10天,患者诉头晕、疲劳、乏力、食欲差,咳嗽时感觉胸骨刺痛不适,无恶心、呕吐、反酸,无腹痛、腹泻等不适。体格检查示体温36.5℃,脉搏90次/分,呼吸16次/分,血压120/80 MMHg,各心脏瓣膜区未闻及病理性杂音,腹部无明显阳性体征。当日嘱生脉注射液(上海和黄药业有限公司,批号为20110914)40 Mg+5%葡萄糖注射液250 Ml静脉滴注,益气养阴。给药约10 MIn,患者突然出现寒战、四肢抽搐、口吐白沫,但神志尚清醒,无眼前黑、呕吐、心前区疼痛等不适。体

pJAK2 polypeptide,an antagonist of suppressors of cytokine signaling-1,can enhance the antitumor effect of dendritic cells

目的:探讨细胞因子信号转导抑制因子-1(SuPPrESSOrS Of CyTOkInE SIgnAlIng1,SOCS1)拮抗物PJAk2多肽(氨基酸序列号为1001-1013)参与树突状细胞(dEndrITIC CEllS,dCS)的体外诱导培养后对dCS抗肿瘤作用的影响。方法:采集健康人外周血,离心获得单个核细胞,用重组人粒细胞巨噬细胞集落刺激因子(rECOMbInAnT HuMAn grAnulOCyTE-MACrOPHAgE COlOny STIMulATIng fACTOr,rHgM-CSf)及重组人白细胞介素-4(rECOMbInAnT HuMAn InTErlEukIn-4,rHIl-4)诱导dCS,第5天分为4组:单纯dCS培养(对照)组、抗原负载(lySATE-dCS)组、多肽修饰(PJAk2-dCS)组和抗原与多肽共培养(lySATE+PJAk2-dCS)组,第6天各组加入肿瘤坏死因子-α(TuMOr nECrOSIS fACTOr-AlPHA,Tnf-α)促成熟。倒置显微镜下观察dCS形态;fCM法检测dCS表型;乳酸脱氢酶(lACTATE dEHydrOgEnASE,ldH)细胞毒实验检测各组细胞毒性T淋巴细胞(CyTOTOXIC T lyMPHOCyTE,CTl)对胃癌细胞bgC-823的靶向杀伤作用;ElISA法检测白细胞介素-12(InTErlEukIn-12,Il-12)和γ干扰素(InTErfErOn-γ,Ifn-γ)的水平。结果:与未加入诱导剂组相比,各组均成功诱导出成熟dCS,均高表达Cd80、Cd83、Cd86和人类白细胞dr抗原(HuMAn lEukOCyTE AnTIgEn dr,HlA-dr),但以lySATE+PJAk2-dCS组的表达水平最高。在10:1~30:1的效靶比范围内,CTl杀伤作用与效靶比呈正相关。当效靶比为30:1时,对照组的CTl杀伤率达(19.77±2.34)%,低于其他3组(P<0.01),而lySATE+PJAk2-dCS组较lySATE-dCS组及PJAk2-dCS组都高(P<0.05)。lySATE+PJAk2-dCS组培养上清液中Il-12及Ifn-γ的分泌水平明显高于对照组(P<0.01)。结论:SOCS1拮抗物PJAk2多肽(1001-1013)可增强dCS对胃癌细胞的抗原递呈及特异性抗肿瘤作用。Objective:To investigate the effect of pJAK2 polypeptide,an antagonist of SOCS1(suppressors of cytokine signaling 1),on antitumor effect of in vitro cultivation-induced DCs(dendritic cells).Methods:Peripheral blood was collected from the healthy volunteers,and the PBMCs(peripheral blood mononuclear cells)were isolated.DCs were induced by rhGM-CSF(recombinant human granulocyte-macrophage colony-stimulating factor)and rhIL-4(recombinant human interleukin-4).On the fifth day,DCs were divided into four groups:control group,Lysate-DCs group,pJAK2-DCs group,and Lysate + pJAK2 DCs group.On the sixth day,TNF-α(tumor necrosis factor-alpha)was added into each group.The morphological features of DCs were observed under an inverted microscope;the phenotypes were detected by FCM(flow cytometry);the killing effect of CTLs(cytotoxic T lymphocytes)on gastric cancer BGC-823 cells was evaluated by LDH(lactate dehydrogenase)cytotoxicity test;the concentrations of IL-12(interleukin-12)and IFN-γ(interferon-γ)were detected by ELISA(enzyme-linked immuno sorbent assay).Results:Mature DCs presented typically morphological and phenotypic features;the DCs in Lysate + pJAK2-DCs group had the highest expression levels of CD80,CD83,CD86 and HLA-DR(human leukocyte antigen DR).When the ratio of effectors to target cells ranged from 10:1 to 30:1,the killing activity of CTLs had a positive correlation with the ratio.When the ratio of effectors to target cells was 30:1,the killing activity of CTLs in the control group was(19.77±2.34)%,which was lowest as compared with the other groups(P < 0.01),meanwhile the killing activity of CTLs in Lysate + pJAK2-DCs group was higher than those in Lysate-DCs and pJAK2-DCs groups(P < 0.05).The levels of IL-12 and IFN-γ secretion in Lysate + pJAK2-DCs group were apparently higher than those in the control group(P < 0.01).Conclusion:An antagonist of SOCS1,pJAK2 polypeptide,can enhance the ability of antigen presentation and specific antitumor effect of DCs on gastric cancer cells.南京军区医学科技创新课题资助项目(编号:10MA068); 福建省自然科学基金面上项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014

Effect of Helicobacter pylori on the function of peripheral blood monocyte-derived dendritic cells in gastric cancer patients

分析HP阳性和HP阴性的胃癌患者外周血单核细胞来源树突状细胞(MOdCS)功能的差异性及其临床意义。方法:用尿素14C呼气试验对解放军第一七四医院2011年1月至2012年10月收治的84例胃癌患者进行HP感染状态鉴定,分别采集HP阳性和阴性胃癌患者外周血,分离外周血单个核细胞,采用经典方法(rHgM-CSf、rHIl-4联合rHTnf-α)诱导产生dCS,采用流式细胞仪检测dCS表型,采用ldH释放法检测dCS致敏T细胞对胃癌细胞的毒性杀伤作用,采用ElISA方法检测细胞因子Il-12、Ifn-γ的分泌水平。结果:两组MOdCS成熟过程形态变化无差异,HP阳性组MOdCS表面标记分子Cd1A、Cd80、Cd83、Cd86和HlA-dr平均表达百分率均高于HP阴性组,其中Cd80、Cd83、Cd86的表达水平差异有统计学意义,Cd1A、HlA-dr差异无统计学意义。HP阳性组dCS致敏T淋巴细胞对胃癌细胞杀伤率和Il-12、Ifn-γ的分泌水平均高于HP阴性组(P<0.05)。结论:HP感染状态不影响胃癌患者外周血MOdCS成熟过程形态变化,HP感染的胃癌患者MOdCS成熟和活化水平更高。Objective:This study aimed to compare and analyze the functional differences between peripheral blood monocyte-derived dendritic cells(DCs) of Helicobacter pylori-positive and H.pylori-negative patients with gastric cancer.Methods:H.pylori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the14C-urea breath test.DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhIL-4,rhGM-CSF,and rhTNF-α.Furthermore,the expression of surface marker molecules was determined by fluorescence-activated cell sorting analysis.The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydrogenase-releasing assay.The secretion of IL-12 and IFN-γ in the supernatant was determined by enzyme-linked immunosorbent assay.Results:No difference was observed in the morphological change of the maturation process.The mean expression of CD1a,CD80,CD83,CD86,and HLA-DR molecules in DCs of H.pylori-infected patients was higher than that in DCs of H.pylori-negative group,and the differences were statistically significant except for CD1a and HLA-DR.The cytotoxicity activities,IL-12 release,and IFN-γ release in the H.pylori-positive group were significantly higher than those in the H.pylori-negative group(P<0.05).Conclusion:H.pylori infection has no effect on the morphological change of the maturation process of monocyte-derived DCs.These data clearly demonstrate that monocyte-derived DCs of H.pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H.pylori-negative patients.南京军区医学科技创新课题(编号:10MA068); 福建省自然科学基金资助项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014)资助~

冷却储存环主环磁铁的准直测量

采用激光跟踪仪及配套带软件Insight对兰州重离子加速器冷却储存环主环磁铁进行了安装和准直。首先建立全局坐标系,用来确定从源体到实验环各子系统的理论原点的位置;而各个子系统又分别以各自的理论原点为基础建立局部坐标系,用来安装定位本系统内的各个元件;对各个子系统中的每一个元件还采用了元件坐标系。准直测量时,先在每块磁铁上焊接8个测量基准;然后对磁铁上的基准进行测量,以确定其在元件坐标系中的位置;再架设激光跟踪仪,测量主环控制网点,恢复主环局部坐标系,根据元件在主环局部坐标系中的位置及理论坐标,计算出相关的变换参数;转入元件坐标系,采用激光跟踪仪及Insight软件显示实时测量坐标及其与理论值之差,其精度达到0.15mm

福建省动物学学科发展研究报告

1引言动物学是一门具多分支学科的基础科学,不仅学科本身的理论研究内容广博,与农、林、牧、渔、医、工等多方面的实践也有密不可分的关系。纷纭多彩的动物界不仅为人类的衣、食、住、行提供了宝贵资源,也为美化人们的生活、满足人们精神生活的需要提供了丰富内容