银鲫果糖-1,6-二磷酸酶的分子克隆与表达分析

果糖-1,6-二磷酸酶(EC 3.1.3.11)是糖异生中的关键限速酶之一,在糖代谢中起重要作用。哺乳动物存在肝脏型和肌肉型两种果糖-1,6-二磷酸酶同工酶,分别由Fbp1和Fbp2编码。银鲫作为我国重要的经济养殖鱼类,尚无果糖-1,6-二磷酸酶基因的有关资料,其组织分布特征和胚胎发育模式亦不清楚。本研究采用RACE方法从银鲫原肠胚SMART cDNA文库中扩增了果糖-1,6-二磷酸酶基因的全长cDNA,其长度为1170 bp,编码337个氨基酸残基,多重序列比对和系统发育分析表明该基因为肝脏型果糖-1

CuInS_2 Quantum Dot-Sensitized Solar Cells Fabricated via a Linker-Assisted Adsorption Approach

分别以Cu I和In AC3作为铜源和铟源,十二硫醇(ddT)作为硫源,采用直接加热法合成不同尺寸的Cu In S2(CIS)量子点.运用X射线衍射(Xrd),拉曼光谱(rAMAn),高分辨率透射电镜(HrTEM),紫外-可见(uVVIS)吸收光谱表征其相结构、形貌及光学性能.结果表明:制备的CIS量子点为黄铜矿结构,且随着时间的延长,量子点逐渐长大,吸收光谱的激子吸收峰逐渐红移,表现出量子尺寸效应.采用巯基乙酸为双功能耦联剂辅助吸附法制备CIS敏化的TI O2薄膜.通过衰减全反射红外光谱(ATr-fTIr)分析得出,巯基乙酸上的羧基与TI O2表面羟基连接,另一端上的巯基代替长链的ddT与CIS耦联,将CIS成功锚定在TI O2表面.该方法不仅操作简单,而且容易实现CIS在TI O2表面的吸附.太阳电池光电性能测试表明,粒径大小约为3.6 nM的CIS量子点表现出最优的吸附能力以及光电转换性能.进一步采用连续离子吸附层法对CIS敏化的TI O2薄膜进行Cd S包覆,光电转换性能大大提高,其效率达到2.83%,这主要源于Cd S的包覆钝化了CIS的表面缺陷,有效地降低了电子复合.Colloidal chalcopyrite CuInS2(CIS) quantum dots(QDs) were synthesized using copper(I) iodine(CuI) and indium(III) acetate(In Ac3) as metal cationic precursors, and dodecanethiol(DDT) as the sulfur source and solvent.The microstructure and optical properties of the prepared CIS QDs were characterized by X-ray diffraction(XRD), Raman spectroscopy, high-resolution transmission electron microscopy(HRTEM), and UVVis absorption spectroscopy.The results showed that the CIS consisted of chalcopyrite phase and exhibited Cu-Au ordering.With prolonged reaction time, the grain sizes of the QDs became larger and the absorption edges of the CIS QDs showed a red-shift owing to the size-induced quantum confinement effect.For the first time, DDT-capped CIS QDs with narrow size distribution were connected to the inner surface of mesoporous Ti O2 films via a thioglycolic acid(TGA)-assisted adsorption approach, which was simple and easy to carry out.The adsorption behaviors of both TGA and the CIS QDs on the Ti O2 films were detected by attenuated total reflectance Fourier transform infrared(ATR-FTIR) spectroscopy.The results indicated that TGA was adsorbed onto the surface of Ti O2 via COOH groups while the ―SH group was exposed outside, and replaced DDT at the surface of the CIS QDs, leading to the attachment between Ti O2 and CIS.It was revealed that the CIS QDs of ~3.6 nm in size exhibited the best light absorption capacity and photovoltaic performance.An over-coating of Cd S significantly improved the performance of the QDSSCs owing to decreased electron recombination, and a power conversion efficiency of ~2.83% was obtained.supportedbytheNationalNaturalScienceFoundationofChina(21073193;21273241;21376195); ProjectontheIntegrationofIndustry;EducationandResearchofGuangdongProvince;China(2012B091100476); ScienceandTechnologyResearchProjectofGuangzhou;China(2014J4100218)~

斑马鱼窖蛋白-1基因cDNA克隆及功能初步研究

窖蛋白-1(Cav-1)是胞膜窖的主要结构蛋白,可与多种信号分子相互作用,调节细胞的增殖、分化和凋亡,其异常表达与多种人体疾病的发生和发展密切相关,而在斑马鱼发育中的功能尚不很清楚。研究克隆出斑马鱼窖蛋白-1基因两个亚型的全长cDNA,与其他物种窖蛋白-1的氨基酸序列进行比较,发现该蛋白在脊椎动物中非常保守。利用逆转录多聚酶链反应检测发现,在斑马鱼多个成年组织中窖蛋白-1的两个亚型均有转录表达。利用胚胎整体原位杂交检测组织或器官特异基因的时空表达变化发现,过表达或利用Morpholino反义寡聚核苷酸(

典型重金属离子印迹技术

分子印迹技术(molecular imprinting technology,MIT)是指制备对特定目标分子具有专一识别性能的聚合物技术。离子印迹技术(ion imprinting technology,IIT)作为分子印迹技术的重要分支,因存在金属离子配位作用而具有很多优势,近年来得到了快速的发展。本文介绍了离子印迹技术原理、制备方法及其在金属的痕量和超痕量分析时的应用,并针对海洋环境监测中非常受关注的典型重金属污染离子(铅、汞、铜、镉、铬、砷)的主要研究进展进行了综述,对其未来的发展做了展望

Propagated error analysis of digital elevation models generated by bi-cubic hermite interpolation methods

2013-2014 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Research on The Application of Typography in Multi-media Circumstance

本文以多元媒介环境中的文字设计为研究对象，结合该背景下媒介、受众等诸多因素的变化，分析了当前文字设计视觉表现的多元化与审美体验互动化的典型趋势。论文首先对新媒介、多元媒介环境及文字设计等概念进行梳理，并就其特征展开分析、归纳与总结，明晰了新媒介是以现代科技为基础的媒介统称，多元媒介环境是指新的媒介发展对社会结构产生的新面貌，文字设计则是指以文字为元素的设计统称。其次，重点就多元媒介环境下文字设计的应用与新原则展开论述，重点分析与阐述了多元媒介环境在信息等诸多方面体现出的与传统媒介环境所不同的互动性、多元化、开放性、娱乐性等特征。最后，就该命题之创作理念与实践展开分析与评价。 其中，在多元媒介...The typography of multi-media environment is the object of the study in this paper, analyzes the typical trend of the diversification and aesthetic experience of the visual performance of typography combined with the changes between media and audiences in the multi-media circumstance. Firstly, the paper introduces the concept of new media, multi-media environment and typography, and analysis, indu...学位：艺术学硕士院系专业：艺术学院_设计学学号：1862014115362

Mongolian laws and their adaptation in early Qing period : with a focus on the Shunzhi era (1644-1661)

PDF/A形式により利用可能アクセス:WWWによる東京外国語大学大学院総合国際学研究科博士 (学術) 論文 (2016年3月)博甲第210号その他のタイトルは英文要旨による参考文献一覧: p157-162東京外国語大学 (Tokyo University of Foreign Studies)博士 (学術

Synthesis and Characterization of Flattened CNTs and Graphene Nanoribbons and the FET-Device Fabrication

名古屋大学Nagoya University博士(理学)doctoral thesi