Study on Highly Stiffness and Lightweight Sandwich Panel Structure using Origami/Kirigami Engineering

埼玉工業大学博士（工学）2023doctoral thesi

Anti-demodecidosis activity and skin safety ofJatropha curcas.1 leaves extract in vitro

目的 探讨麻风树叶治疗蠕形螨病的应用价值。方法 用80%乙醇热回流提取法提取麻风树叶提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设不同浓度麻风树叶实验组、2%浓度的甲硝唑对照组和生理盐水对照组,进行体外抗螨实验。pH仪测定不同浓度麻风树叶提取物pH值。设麻风树叶实验组和75%乙醇对照组,用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果 50、25、12 mg/ml麻风树叶组与2%甲硝唑组毛囊蠕形螨死亡时间分别为（1.55±0.67）min、（1.61±0.67）min、（2.47±0.80）min和（1.20±0.48）min。50、25 mg/ml麻风树叶组以及2%甲硝唑组两两之间差异无统计学意义（P均〉0.05）。50、25、12 mg/ml麻风树叶提取液pH值分别为6.07±0.73、6.27±0.82、6.35±0.83,对家兔完整皮肤及破损皮肤刺激评分均为0,且无明显毒性。结论 麻风树叶提取物具有较强的体外抗蠕形螨活性且具有皮肤安全性。Objective To test the application value of the extract of Jatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.1 leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen from demodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups of Jatropha curcas.l leaves, 2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations of Jatropha curcas.l leaves extract was determined. Skin irritation test and acute skin toxicity test were carried out in healthy rabbits, and Jatropha curcas.1 leaves and 75% ethanol served as experiment and control groups, respectivelyl Results The mite-killing time was （1.55-4-0.67） min with 50 mg/ml Jatropha curcas.l leaves extract, （1.61-4-0.67） min with 25 mg/ml Jatropha curcas.l leaves extract, （2.47-4-0.80） min with 12 mg/ml Jatropha curcas.l leaves extract, and （1.20-4-0.48） min with 2% metronidazole. There was no significant different between 50, 25 mg/ml Jatropha curcas.l leaves extract groups and 2% metronidazole group （P 〉 0.05）. The pH value was 6.07＋0.73 of 50 mg/ml Jatropha curcas.l leaves extract, 6.27-1-0.82 of 25 mg/ml Jatropha curcas.l leaves extract and 6.35-4-0.83 of 12 mg/ml Jatropha curcas.1 leaves extract. Score for irritation to normal and wounded rabbit skin was both 0, and acute toxicity test showed no significant toxicity. Conclusions Jatropha curcas.l leaves extract shows a remarkable activity to demodecidosis with skin safety in vitro.漳州市自然科学基金（ZZ2014J33

玻碳电极表面复合配位银电结晶机理研究

以具有实际应用价值的复合配位体系无氰镀银电解液为研究对象,运用循环伏安和电位阶跃等实验方法,结合Scharifker-Hill经典理论模型分析,成功获得了Ag在玻碳电极(GCE)表面电沉积的成核机理及成核动力学参数,并分析了温度对成核方式及成核动力学参数的影响.结果表明,该体系下Ag在GCE表面的电沉积是由扩散控制的不可逆过程,遵循三维瞬时成核生长机理.随着阶跃电位从-750 m V负移至-825 m V,峰值还原电流Im逐渐增大,达到峰值还原电流所需时间tm逐渐缩短,扩散系数D变化不大,基本稳定在(7.61±0.34)×10-5cm2·s-1,成核密度数N0则从3.26×105cm-2提高至10.2×105cm-2.银沉积初期的形貌观察,验证了其三维瞬时成核生长机理.提高温度可以显著改善电解液中具备活性的银配位离子的扩散能力,缩短成核时间,提升成核密度数N0.国家自然科学基金项目(No.21621091)资

Comparison of the preparation methods of Temozolomide-loaded nanoparticles

目的比较载替莫唑胺聚氰基丙烯酸正丁酯纳米粒(TMz-PbCA-nP)的不同制备方法,确定最佳制备工艺。方法以α-氰基丙烯酸正丁酯(bCA)为载体,分别采用乳化聚合法和界面聚合法制备TMz-PbCA-nP,加以吐温-80(T-80)进行表面修饰,并通过zETA电位仪检测纳米粒粒径和电位、透射电镜观察纳米粒形态、紫外分光光度计测定各自的包封率和载药量。结果乳化聚合法制备的TMz-PbCA-nP平均粒径(135.8±11.3)nM,表面电位(-24.8±2.2)MV,包封率(44.23±2.04)%,载药量(2.80±0.05)%;界面聚合法制得的载药纳米粒平均粒径(175.4±10.2)nM,表面电位(-18.3±3.6)MV,包封率(44.35±2.58)%,载药量(2.31±0.47)%。透射电镜下观察两种方法所制备的纳米粒大小均较为均匀,粒子间无明显聚集。结论采用乳化聚合法制备TMz-PbCA-nP效果较优于界面聚合法。【Objective】 To compare Temozolomide polybutylcyanoacrylate nanoparticles(TMZ-PBCA-NP) prepared by two different methods so as to determine the optimal process.【Methods】 TMZ-PBCA-NP was prepared by emulsion polymerization and interfacial polymerization methods separately and the surfaces of the two kinds of nanoparticles were both modified with tween-80(T-80).Zeta potential instrument was used to detect the particle size and charge and transmission electron microscopy was employed to study the particle shape.The entrapment efficiency and drug loading were determined with ultraviolet spectrophotometry.【Results】 The mean particle size,surface charge,entrapment efficiency and drug loading of the NP prepared by emulsion polymerization and interfacial polymerization methods was(135.8±11.3) nm and(175.4±10.2) nm,(-24.8±2.2) mV and(-18.3±3.6) mV,(44.23±2.04) % and(44.35±2.58) %,(2.80±0.05) % and(2.31±0.47) %,respectively.Both nanoparticles had uniform size distribution and no apparent aggregation according to transmission electron microscopy.【Conclusion】 The emulsion polymerization method is better than interfacial polymerization method in preparing TMZ-PBCA-NP.国家自然科学基金(No:81172394

Dynamic changes of phytoplankton ' s community structure in Beixi of Jiulongjiang River,Fujian Province of East China and related affecting factors

分别于2011年枯水期(2月)、丰水期(5月)和平水期(10月),系统调查研究了福建九龙江北溪浮游植物群落组成、丰度的分布特征及其与环境因子的关系.共鉴定浮游植物107种,隶属于7门64属.不同水文期浮游植物主要优势种类不同,枯水期为马索隐藻和梅尼小环藻,丰水期为四尾栅藻和四角十字藻,平水期则演替为微小平裂藻.不同水文期浮游植物丰度变化明显,其平均值依次为枯水期(154.77x104CEllS.l-1)>平水期(76.40x104CEllS.l-1)>丰水期(45.40x104CEllS.l-1).相关分析表明,枯水期和平水期浮游植物丰度与铵态氮(nH4+-n)呈显著正相关,丰水期浮游植物丰度与温度呈极显著正相关.典范对应分析(CCA)表明,水体温度是影响该水域浮游植物分布格局的重要因子,溶解态活性磷浓度也对浮游植物的分布有较大的影响.CCA排序图较好显示了浮游植物物种分布和环境因子之间的关系.An investigation was conducted on the phytoplankton's community composition,abundance,and their relations with environmental factors in Beixi of Jiulongjiang River,Fujian Province in February(dry season),May(wet season),and October(normal season),2011.A total of 107 phytoplankton species were identified,belonging to 64 genera and 7 phyla.The dominant species changed with seasons.Campylomonas marssoni and Cyclotella meneghiniana dominated in dry season,Scenedesmus quadricauda and Crucigenia tetrapedia dominated in wet season,and Merismopedia tenuissima dominated in normal season.The phytoplankton's abundance was the highest in dry season(averaged 154.77×104 cells·L-1),followed by in normal season(76.40×104 cells·L-1),and in wet season(45.40×104 cells·L-1).The abundance in dry and normal seasons was significantly positively correlated with water ammonium nitrogen concentration(P<0.05),while that in wet season was significantly positively correlated with water temperature(P<0.01).Canonical correspondence analysis(CCA) showed that water temperature was the most important factor affecting the phytoplankton's community structure,and dissolved phosphorous concentration also played important role for the community structure.The CCA ordination plots could well display the phytoplankton's community structure and its relationships with environmental factors.厦门市重大科技平台项目(3502Z20091005)资

Preparation of fotemustine-loaded polybutylcyanoacrylate nanoparticles

目的优化工艺制备福莫司汀聚氰基丙烯酸正丁酯纳米粒(fCnu-PbCA-nP)。方法以α-氰基丙烯酸正丁酯(bCA)为载体,采用乳化聚合法制备fCnu-PbCA-nP,并加以聚乙二醇20000(PEg20000)进行表面修饰,通过考察粒径和包封率两个指标,在单因素实验初选的基础上,正交设计法优化处方和制备工艺。结果制备fCnu-PbCA-nP的优化条件为bCA单体体积分数0.8%(V/V)、fCnu 20 Mg、PEg20000浓度2.0%,按优化条件所制备的fCnu-PbCA-nP的粒径为(124.6±5.2)nM,多分散系数(PdI)范围为0.07--0.16,包封率(64.12±2.36)%,载药量(7.28±0.76)%。结论通过优化处方和制备工艺,采用乳化聚合法可制备出fCnu-PbCA-nP,对拓展fCnu临床给药新剂型提供一定的参考。AIM To prepare fotemustine polybutylcyanoacrylate nanoparticles(FCNU-PBCA-NP) with optimized process.METHODS FCNU-PBCA-NP was prepared by emulsion polymerization with the α-butylcyanoacrylate(BCA) as its carrier and the surface of the nanoparticles was modified with polyethylene glycol 20000(PEG20000).Single factor test and orthogonal design were carried out to optimize the preparing technology according to the particle size and the entrapment efficiency of FCNU-PBCA-NP.RESULTS The optimal conditions for the preparation of FCNU-PBCA-NP were 0.8% BCA monomer(V/V),20 mg fotemustine and 2.0% PEG20000(m/V).On the basis of the above conditions,the mean particle size of the NP was(124.6±5.2)nm and the polydispersity index(PDI) was 0.07-0.16,the average entrapment efficiency and drug loading was(64.12±2.36)% and(7.28±0.76)%,respectively.CONCLUSION An optimized nanoparticle drug delivery system is obtained by emulsion polymerization and provides a new direction for fotemutine dosage forms in future.福建省自然科学基金(2006J0188);厦门市科技局基金(3502Z20064013

红树林土壤中脂肪酶产生菌的筛选及酶学性质

从福建龙海红树林区土壤中分离获得9株产脂肪酶的细菌,经16S rDNA序列分析表明分别属于红球菌属(Rhodococcus)、库克菌属(Kocuria)、假单胞菌属(Pseudomonas)、芽胞杆菌属(Bacillus)和微小杆菌属(Exiguobacteri-um).对9株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在25~35℃、最适作用pH值在8~10,其中L13菌株所产脂肪酶(L′13)具有适应温度和pH范围较广、对多种金属离子耐受性强、能有效降解不同底物等特点,在环境保护和工业生产方面具有良好的应用前景

微生物絮凝剂对小球藻细胞氧化应激研究

小球藻作为能源藻,可以提供产生物柴油,但是小球藻生物质的获取成为能源藻发展的瓶颈.目前研究藻细胞生物质收集的方法很多,但不同收集方法对能源藻细胞的氧化应激研究却很少.分别研究絮凝功能细菌xn-1所产生物絮凝物质和离心法对能源藻——小球藻生物质在收集过程中对藻细胞的氧化应激,以期确定絮凝微生物对小球藻细胞的生物安全性.采用涂布划线法从藻际分离纯化絮凝功能微生物;通过梯度醇沉法获得絮凝物质;分别用絮凝微生物所分泌絮凝物质和离心法收集藻细胞生物质,并对藻细胞内蛋白含量、总糖含量、丙二醛含量(MDA)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和抗坏血酸(AsA)含量进行测定;以分光光度计测定其吸光度值.经过测定并经过统计学显著性分析,发现离心作用下收集的藻细胞内的MDA,GSH和AsA含量显著高于利用絮凝物质絮凝得到的藻细胞.离心作用相对于絮凝作用造成藻细胞膜发生更高的脂质过氧化,产生大量的活性氧自由基,诱导非酶抗氧化系统响应.因此,离心法收集藻生物质相对于微生物絮凝对藻细胞的伤害更大,絮凝微生物比离心法更适合于收集能源藻生物质.国家自然科学基金(51008119;41576109);;河南省高等学校重点科研项目计划(17A610002

粗糙铂电极上甲酸吸附氧化的电化学原位表面增强拉曼光谱研究

采用循环伏安法和电化学原位表面增强拉曼光谱 (SERS)技术研究甲酸的解离吸附与氧化行为 .首次报道了甲酸吸附、解离和氧化的电化学原位SERS谱 ,发现甲酸在粗糙铂电极上能自发解离吸附 ;首次成功地获得了粗糙铂电极上甲酸吸附解离的强吸附中间体CO和活性中间体COOH的表面增强拉曼光谱 ,同时首次检测到甲酸氧化最终产物CO2 的拉曼光谱信号 ,从分子水平证实甲酸解离吸附反应的双途径机理