The preparation and biological activity research of Gypenoside of Gynostemma pentaphyllum and its metaboliezd product by microorganic transformation

绞股蓝[Gynostemmapentaphyllum(Thunb)Makino]为葫芦科多年生落叶草质宿根藤本植物，是目前已知的除五加科植物人参属以外，唯一含有人参皂苷类成分的植物。大量研究表明，绞股蓝皂苷在抗氧化、抗炎、抗癌、改善免疫力等方面都表现出较高活性，因此绞股蓝一直都是研究开发中备受关注的药用植物。 本论文是从绞股蓝中提取绞股蓝总皂苷，通过微生物转化，切除绞股蓝皂苷侧链的糖苷，从而获得经过微生物代谢后的次级绞股蓝皂苷。并比较研究转化前后绞股蓝总皂苷的生物学效应。主要研究内容及结果如下： 比较水浴加热提取与超声提取两种方法对绞股蓝总皂苷提取效率的影响，结果表明超声法提取率高于水浴加...Gynostemma pentaphyllum is herbaceous perennial root liana of cucurbitaceae of perennial and deciduous. And it is known as the only plant which contain ginsenosides besides the Panax of Araliaceae. Much researchs reveal that gypennosides show obvious effect on anti-oxidation, anti-inflammation, anti-cancer and immunity improvement. So Gynostemma pentaphyllum has always been paid attention in resea...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2172009115207

现代民间金融的政策与思考

民间金融活动 (或组织 )是一种复杂的经济金融甚至社会现象。它既是一种提供资金融通服务的经济制度 ,又具有促进社会进步的社会制度的特征 (就象孟加拉的乡村银行那样 )。民间金融的复杂性质要求我们在关于民间金融发展的金融政策上应当保持足够的灵活性

信贷配给与货币政策

在货币理论中,无论是凯恩斯学派还是货币主义学派,都注重货币市场的作用而忽视金融机构和信贷市场的影响。在凯恩斯的通论中,流动性偏好理论完全取代了可贷资金理论以及金融机构和公司财务结构的作用。后来被纳入到希克斯的IS-LM框架

公共利益本位视角下城乡规划变更实现机制探究

随着城市快速发展,城乡规划变更面临诸多问题,涉及土地价值、性质以及相关利益关系的重新分配,从而引发相关利益群体不同程度的利益\"受损\"或\"受益\"。基于公共利益本位,探讨规划变更实现的关键性问题,提出在规划变更过程中,利益相关人的参与有利于对规划行政主体进行监督与约束,从而实现行政权与公民权的\"平衡\";行政主体对\"受损\"的个人利益进行合理补偿,以实现公共利益与个人利益的\"平衡\",为我国城乡规划变更得以顺利实现提供可行思路

Microbial Transformation of Gypenoside from Gynostemma pentaphyllum by Aspergullus glaucus and Its Biological Activities

以福建绞股蓝[Gynostemma pentaphyllum(Thunb)Makino]为材料,经乙醇提取和正丁醇萃取获得绞股蓝皂苷,利用灰绿曲霉(Aspergullus glaucus)微生物转化绞股蓝皂苷,通过测定绞股蓝皂苷和灰绿曲霉转化产物的抗癌、抗酪氨酸酶和抗氧化活性,比较绞股蓝皂苷经微生物转化修饰前后生物活性的差异.结果表明:灰绿曲霉转化产物对肝癌细胞SMMC7721有体外抑制作用,半抑制质量浓度(IC50)为91.66μg/m L,而绞股蓝皂苷抗癌效果不强;绞股蓝皂苷对蘑菇酪氨酸酶活力具有较强的抑制作用,IC50为0.14 mg/m L,其抑制作用属于可逆抑制,抑制类型为混合型抑制作用,而灰绿曲霉转化产物抗蘑菇酪氨酸酶活性较弱;绞股蓝皂苷和灰绿曲霉转化产物分别在0.2和2 mg/m L质量浓度下对DNA氧化损伤有保护作用.研究结果说明绞股蓝皂苷经微生物修饰后,抗癌效果增强,对蘑菇酪氨酸酶抑制作用和DNA氧化损伤保护作用减弱.该研究结果可为利用微生物转化法筛选抗癌绞股蓝皂苷奠定基础.Gynostemma pentaphyllum in Fujian Province was used as the material in this research.Gypenoside was extracted with ethanol and n-butyl alcohol. Gypenoside was microbially transformed by Aspergullus glaucus. The abilities of anticancer,anti-tyrosinase and anti-oxidation in gypenoside and transformation products were measured in our study.The difference of bioactivity between gypenoside and microbially transformed gypenoside was compared.The result showed that the products of transformation by A. glaucus affected the growth of hepatocellular carcinoma cells SMMC7721 with half maximal inhibitory concentration( IC50) values of 91. 66 μg/m L,but the anticancer activity of gypenoside was very weak.Gypenoside had strong inhibition to the mushroom tyrosinase. The value of IC50 was 0. 14 mg / m L. Moreover,the products of transformation by A. glaucus showed little anti-tyrosinase acti-vity.The kinesis study showed that the inhibition of gypenoside to mushroom tyrosinase was reversible and inhibition types were mixed. Gypenoside exhibited the active protective effect on H2O2-induced oxidative-stress damage at the concentration of 0. 2 mg / m L,and the products of transformation by A. glaucus were at 2 mg / m L. This research indicated that the anticancer effect of gypenoside modified by microorganisms was enhanced,but the activity of anti-tyrosinase and H2O2-induced oxidativestress damage became weakened.This study laid the foundation of screening anticancer gypenoside by means of microbial transformation.国家自然科学基金(81274149);; 厦门市科技创新项目(3502Z20132009,3502Z20154083);; 厦门市对台科技合作项目(3502Z20141041

学生ピアリーダーの成長プロセスとその要因分析に関する質的研究 : 立命館アジア太平洋大学のティーチング・アシスタントへのインタビューをとおして

departmental bulletin pape

A case study of cumulative effects assessment for harbor planning in China

累积影响评价是战略环境评价的主要方法和重要内容之一,但目前还缺乏通用的评价程序和方法。通过识别港口规划的累积影响源和途径,建立了基于水动力数值模型的累积影响评价的量化评估和分析框架。以厦门港嵩屿港区规划为例,通过对海湾纳潮量及各敏感点流速的模拟,计算淤积量及其分布变化,进而推测海域水质和底质变化,并分析了由此引起的海洋生物群落的变化趋势。通过比较不同岸线方案的累积影响,为港区岸线优选提供了科学判据。实践表明,该方法框架综合运用定量预测和定性分析手段,其结果能为港口规划的战略环境评价提供科学依据。 【英文摘要】 Cumulative effects assessment (CES) has become an important approach and also a key component of the strategic environmental assessment due to the increased recognition that most significant environmental changes are results of combined effects of many individual impact factors. Analyzing combined environmental effects is simple in principle but difficult in practice due to a lack of simple and effective CES methods and procedures. The paper presents a case of CIE for the harbor planning project for Songyu ..

Current status of and problems in medical treatment for newborn infants in our hospital

1995年10月より2003年1月までの間に当院で入院加療した新生児480名を対象とし、新生児医療の現状と問題点を検討した。院外出生の割合は21.5%で三次医療機関への搬送は全体では16,9%であった。低出生体重児は38.1%で、早産児は28.9%であった。Apgar score1分後7点以下の仮死の割合は24.6%であった。診断名は呼吸窮迫症候群(RDS)、胎便吸引症候群(MAS)などの呼吸器疾患が最も多く、心疾患、感染症が続いた。気管内挿管は9.5%に、サーファクタント投与は6.7%に行われていた。三次医療機関と異なり、新生児特定集中治療室管理料加算が取れない当院では経済的にきびしい側面があり、一般病院の限られた人員と条件のなかで診療を行っている実体が明らかになつた