Design and Implementation of Firefighting Supervision Management Information System

随着我国社会经济的迅猛发展，消防监督管理工作面临着更新的要求与挑战。现今的消防监督管理应该走出传统的管理模式，向现代的网络模式转变。 基于以上原因，本文设计并实现了一个消防监督管理信息系统。本文首先对课题研究的背景、现状、意义等进行了探讨；然后对消防监督管理信息系统相关技术作了简要介绍；分析了消防监督管理信息系统的可行性、系统用户及系统功能分析。通过需求分析得出了系统的总体设计，主要包括系统模式设计、系统功能设计、系统数据库设计等；详细了系统的功能模块设计，主要介绍了系统体系结构设计，消防监督管理信息系统模块的设计。接着详细描述了系统部分模块主要功能的实现效果，主要介绍了消防监督管理信息系统...Along with our country social economy rapid development, the fixed asset management faced with demand and challenge. Today's fire intendance management should be out of the traditional management mode, to modern network model transformation. Based on the above reasons, deciding to develop a fire intendance management system, this dissertation firstly explores the background, situation and signif...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201223084

后安然时代的会计准则:原则导向还是规则导向

针对会计准则的原则导向和规则导向问题 ,本文提出一个简单的解释框架。作者认为 ,如果考虑到经济人属性及会计准则的经济后果 ,会计准则规则导向将是一种必然的结果。针对安然事件之后美国国会重视原则导向 ,作者认为这与全社会对会计准则的批评以及国际会计准则理事会的游说有一定的关联度。国家自然科学基金课题“WTO、透明度与我国会计准则的国际化协调” (70 10 2 0 0 9)的阶段性成

磨床主轴热特性分析及热变形补偿策略

针对五轴数控可转位刀片周边工具磨床建立了砂轮-主轴-轴承-主轴箱的主轴部件有限元模型,分析了主轴部件的热源及其发热量的计算公式,通过有限元仿真计算得到磨床主轴的热稳态温度场和热变形量,进行了主轴热特性分析。在不增加温度、位移传感器的基础上提出了有效的主轴热变形补偿策略,实现刀片加工精度达到5μM的精度要求。国家科技重大专项(2010ZX04001-162

Sequence anlysis of rDNA of Anisakid nematodes with zoonotic potential from Taiwan strait

目的分析台湾海峡异尖线虫病潜在病原线虫的rdnA序列,为建立病原分子生物学鉴别提供依据。方法剖检台湾海峡常见鱼类的寄生线虫幼虫,镜检分类。提取虫体dnA、用通用引物对rdnA进行PCr扩增、克隆、双向测序及blAST比对。结果获得6条来自6种线虫幼虫的rdnA序列,其中的3种在gEnbAnk数据库中没有发现匹配的序列;证实在台湾海峡分布的灰海鳗对盲囊线虫成虫和疑似其第三期幼虫实属同种;构建了基于ITS-2的序列间内部相似性程度关系树。结论异尖线虫病原幼虫所具有的分子生物学鉴别信息位点主要集中在ITS-2间隔区。To analyze the rDNA sequence of the Anisakid nematodes with zoonotic potential from Taiwan straits,Anisakid nematodes were obtained from the gut of marine fish and identified chiefly based on the morphological characteristics.The genomic DNA of nematodes were extracted,and the sequence of rDNA ITS were amplified by PCR using universal primers,then cloned and sequenced bidirectionally.Sequences analysis was conducted by blasting database and software DNAMAN.Results of the blasting database showed that no match could be demonstrated in 3 of 6 rDNA sequences obtained from 6 different nematode species;the new sequences were submitted to the database and the accession numbers in GenBank were obtained.The relationship between adult Contracaecum muraenesoxi and its putative third stage larva was validated by molecular evidences.Sequences relationship based on the inner similarity of the internal transcribed spacer sequences was constructed.It was found that the phylogenetic informative loci were mainly gathered in the second internal transcribed spacer(ITS2) for these anisakid nematodes because of the ITS2 has higher transition ratio than that of the ITS1.It is evident that the sequence in this region could provide valuable information for the molecular distinction of anisakid nematodes with zoonotic potential from Taiwan straits.福建省科技计划项目(2008N2005);厦门市科技计划项目(3502Z20074036

电化学探针技术测量电生刻蚀剂的浓度分布

建立了电化学探针技术检测电生刻蚀剂测量系统，利用该测量系统对在模板表面上电生刻蚀剂溴的微米级浓度分布进行了定量研究

探究白藜芦醇对小鼠皮层神经元兴奋性的影响

目的探究白藜芦醇对小鼠皮层神经元兴奋性的影响。方法取1月龄雄性小鼠制备小鼠急性大脑前额叶皮层切片后,随机分为空白对照组与白藜芦醇组。实验过程中对照组不做处理,白藜芦醇组灌流液加入终浓度为100μmol·L-1的白藜芦醇溶液。采用电生理全细胞记录模式分别记录两组神经元的兴奋性突触后电流（sEPSC）、动作电位（AP）以及Cl-电流。结果与对照组比较,白藜芦醇组兴奋性电流幅度（Amplititude）与频率（Frequency）均降低,梯度电流刺激诱发的AP数目减少,Cl-电流的幅度与频率也明显降低。结论白藜芦醇具有抑制小鼠皮层神经元兴奋性的作用,其机制可能是抑制神经元兴奋性电流的作用优于抑制Cl-电流的作用。国家自然科学基金资助项目(81473740、81673627

Cloning and Expression of L-like Cysteine Protease of Anisakis simplex

目的克隆简单异尖线虫l-样半胱氨酸蛋白酶基因(ASCP)全长,研究其表达特性。方法根据gEnbAnk中简单异尖线虫表达序列标签l-样半胱氨酸蛋白酶基因的部分信息,设计特异引物,用CdnA末端快速扩增技术扩增3′端部分,获得基因全长序列。根据基因全长序列设计引物,以简单异尖线虫总rnA为模板,rT-PCr扩增ASCP基因编码序列,产物经ECOrⅠ和SAlⅠ双酶切,克隆至表达载体PET32а(+),转化大肠埃希菌bl21(dE3)株,以异丙基-β-d-硫代半乳糖苷(IPTg)诱导表达,表达效果经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SdS-PAgE)检测。结果3′端扩增片段大小为1211bP,拼接完整后基因全长1462bP,编码411个氨基酸,与秀丽隐杆线虫的l-半胱氨酸蛋白酶相似性达36.4%;重组载体PET32A(+)-ASCP经ECOrⅠ和SAlⅠ双酶切后有一条约1150bP的条带,测序结果显示重组载体构建成功。SdS-PAgE结果表明,重组蛋白相对分子质量约为Mr60000(含6个组氨酸的标签),与目的蛋白相符。用不同浓度的IPTg诱导对表达量的影响很小,1MMOl/lIPTg诱导2H后表达量达到最高水平。结论成功克隆并表达了l-样半胱氨酸蛋白酶。Objective To clone and express the full lenth of L-like cysteine protease gene of Anisakis simplex (AsCP) .Methods According to L-like cysteine protease encoding gene of A.simplex from GenBank EST database, specific primers were designed to amplify 3′-end of AsCP gene using rapid-amplification of cDNA ends (RACE), and the full lenth of the L-like cysteine protease gene was obtained.Specific primers were designed according to the full length of the gene.Using total RNA of A.simplex third-stage larvae, coding sequence of the AsCP gene was amplified by RT- PCR.The PCR product was digested by EcoR I and Sal I, and cloned into pET32а(+) vector.The recombinant plasmid was checked by double enzyme digestion and sequencing, and the positive recombinant plasmid was transformed into Escherichia coli BL21(DE3).Expression of the protein induced by IPTG of gradient concentration (0.2, 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mmol/L) and by the same concentration (1 mmol/L) of IPTG at different time(0, 1, 1.5, 2, 2.5, 3, 3.5, and 4 h) was conducted.The expression situation of recombinant protein was analyzed by SDS-PAGE.Results A 1 211 bp of 3′-end of AsCP gene was amplified by 3′RACE, full length of the gene was 1 462 bp and coding 411 amino acids.It showed 36.4% identity with the L-cysteine protease of Caenorhabditis elegans.Double enzyme digestion of the constructed recombinant plasimid pET32a(+)-AsCP showed that there was about 1 150 bp fragment, the constructed recom- binant plasmid was then identified by sequencing.SDS-PAGE showed that the recombinant protein (Mr 60 000) was identical with the target.IPTG showed little effect on the protein expression, and the production of protein was up to maximum after 2 hours induction.Conclusion The AsCP gene has been cloned and expressed.福建省科技计划项目(No.2008N2005)---

Detection of Anisakid Nematodes by an SYBR Green Ⅰ Real-time PCR

目的运用荧光定量PCr法检测异尖线虫类病原体。方法于鱼类内脏中检获6种异尖线虫类幼虫:抹香鲸异尖线虫、简单异尖线虫、内弯对盲囊线虫、带鱼针蛔线虫、灰海鳗对盲囊线虫和台湾海峡鱼类中一优势种对盲囊线虫。提取各虫体dnA,PCr扩增ITS-2序列,测序并进行数据库比对。依据测序结果设计特异引物,常规PCr检验引物特异性。将ITS-2序列扩增产物回收、纯化后经T克隆转入大肠埃希菌dH5α,提取重组质粒,鉴定后作为标准品模板建立荧光定量PCr标准曲线,并做敏感性和重复性试验。结果构建的荧光定量PCr标准曲线循环阈值与模板浓度呈良好的线性关系,相关系数均在0.998以上。重复性实验中,6种虫体对应的变异系数(CV)最小值为0.18%,最大值为2.80%,试验间平均CV最小值为0.55%,最大值为1.94%,无非特异性扩增,溶解曲线的特异性和重复性良好。灵敏度实验中,可检出的最低模板浓度为1x102拷贝/μl,比常规PCr灵敏度高100倍。结论初步建立了SybrgrEEnⅠ荧光定量PCr检测异尖线虫类病原体的方法 。Objective To establish an SYBR Green Ⅰ real-time quantitative PCR method for the detection of anisakid nematodes with zoonotic potential from Taiwan Strait.Methods Anisakid larvae of six species (Anisakis simplex, A.physeteris, Raphidascaris trichiuri, Contracaecum aduncum, C.muraenesoxi, and Contracaecum sp., a predominant species in fishes in the strait) were obtained from the guts of marine fishes and identified chiefly based on the morphological features.The ITS-2 rDNA sequences from the larvae were amplified by PCR using universal primers, then cloned and bidirectionally sequenced.According to these sequences, six specific forward primers were designed and synthesized.Specificity was determined by a series of conventional PCR respectively, the ITS-2 sequences amplified above were cloned into T vector which was subsequently transformed into E.coli DH5α.Following extraction and identification, the positive recombinant plasmid was used as quantitative template to generate standard curve and melt curve.Sensitivity and reproducibility were determined.Results All the 6 standard curves established by the recombinant plasmids showed adequate linear relationship between threshold cycle(Ct) and template concentration.Melt curves were specific and all the 6 correlation coefficients were above 0.998.In the reproducibility test, the coefficients of variation (cv) of Ct values for detection of the 6 nematodes ranged between 0.18% and 2.80%, and the cv of the inter-assay ranged between 0.55% and 1.94%.The sensitivity of the real-time PCR was 1×102 copies/μl, about 100 times higher than the conventional PCR assays.The real-time quantitative PCR detection needed only 3.5 hours from the sample treatment to result report.Conclusion An SYBR Green Ⅰ fluorescent quantitative PCR has been developed for detecting anisakid nematodes with adequate sensitivity and specificity.福建省科技计划项目(No.2008N2005)---

Development of Electrochemical Biosensor for Detection of PML/RARα Fusion Gene in Acute Promyelocytic Leukemia

针对急性早幼粒细胞白血病(APl)中PMl/rArα融合基因的碱基序列,设计了锁核酸(lnA)修饰的发夹结构捕获探针,结合信号探针构建新型的“三明治“电化学传感模式。信号探针末端修饰的生物素可与酶上的亲和素结合,通过检测酶催化H2O2氧化底物3,3',5,5'-四甲基联苯胺(TMb)产生的电化学信号,实现对靶序列的检测。该传感器可识别和定量检测PbS缓冲液中人工合成的PMl/rArα融合基因序列。结果表明,该传感器能很好地区分互补序列、单碱基及多碱基错配序列,杂交电流值与目标链浓度在1.0x10-11~1.6x10-10 MOl/l范围内呈较好的线性关系,检出限为1.0x10-13 MOl/l。同时,该新型传感器成功地用于无稀释人血清中PMl/rArα融合基因的检测,具有特异性强、灵敏度高和重复性好的优点,有望用于临床实际样品的检测,进而实现临床上急性早幼粒细胞白血病的早期诊断及预后判断。A novel DNA electrochemical probe(locked nucleic acid,LNA) was designed and involved in constructing an electrochemical DNA biosensor for the detection of PML/RARα fusion gene in acute promyelocytic leukemia(APL).This biosensor was based on a "sandwich" detection strategy,which involved a pair of LNA probes,e.g.hairpin capture probe and reporter probe.Streptavidin-HRP was bound to biotin labeled at the end of reporter probe via streptavidin-biotin affinity binding.In the presence of hydrogen peroxide(H2O2),HRP catalyzed the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidene(TMB) to offer an enzymatically amplified electrochemical current signal for the detection of target DNA.This sensor was applied in the direct quantitative detection of synthetic PML/RARα fusion gene in PBS buffer.The results indicated that the biosensor showed an excellent specificity to distinguish the complementary sequence and different mismatch sequences.A linear relationship between the amperometric signal and the target concentration was obtained in the range of 1.0×10-11-1.6×10-10 mol/L with a detection limit of 1.0×10-13 mol/L.In addition,the biosensor was used for the determination of PML/RARα fusion gene in human serum samples without dilution with high sensitivity,selectivity and good repeatability.This method would be expected to use in real sample for further solving the actural problems of early diagnosis and prognosis monitoring of APL.863计划资助项目(2008AA02Z433);福建省高校产学研科技重点项目(2010Y4003);国家自然科学基金资助项目(20805006;20975021);福建省自然科学基金资助项目(2010J05019

我国高水平游泳运动员运动损伤特点

对 1999年全国游泳冠军赛和锦标赛的 18个省市代表队 12 0名高水平运动员的运动损伤进行全面、系统的调查研究。结果显示 :我国高水平游泳运动员的运动损伤患病率达 6 5 % ;女运动员患病率明显高于男运动员 ;运动员损伤多属于急性中度损伤 ;损伤多发生在冬训和赛前大负荷训练阶段 ;损伤好发部位为肩、腰、膝部 ,以肩袖损伤最多 ;受伤者多为具有 6～ 9年训练年限、运动等级达到健将级水平的 14～ 18岁的运动员 ;损伤的主要原因有超负荷和局部负荷过重、训练水平不够、生理机能或心理状态不良等。福建省教委课题! (NO .JB991345