肛痛消软膏药效学及毒性实验研究

目的:观察肛痛消软膏的镇痛、消肿、促进创面愈合作用及其安全性。方法:通过热板法观察肛痛消软膏的镇痛作用;采用二甲苯引起的小鼠耳廓肿胀观察肛痛消软膏的消肿作用;通过二度烫伤家兔观察肛痛消软膏促进创面愈合作用;观察肛痛消软膏的皮肤刺激反应、皮肤过敏反应和急性毒性反应。结果:肛痛消软膏对热传导引起的疼痛反应有明显的拮抗作用;对小鼠耳廓肿胀有明显的抑制作用;能明显缩短家兔烫伤创面愈合时间;肛痛消软膏对豚鼠完整及破损皮肤无刺激性反应及过敏反应,家兔对肛痛消软膏未出现任何急性毒性反应。结论:肛痛消软膏具有良好的镇痛、消肿、促进创面愈合的作用,是一种安全的中药制剂

Experimental study on effect of Changrun Formula in regulating expression of AQP3 and AQP9 in colon mucosa of functional constipation rats

目的:观察肠润方对功能性便秘模型大鼠结肠黏膜AQP3、AQP9表达的影响,探究其调控AQP3、AQP9表达的分子机制。方法:60只SD大鼠随机分为空白组、模型组、阳性对照组(麻仁丸组)、肠润方组、肠润方联合P38MAPK抑制剂SB203580组。用复方地芬诺酯制造大鼠便秘模型,采用免疫组织化学及实时荧光定量PCR(q RT-PCR)检测大鼠便秘模型近端及远端结肠黏膜AQP3、AQP9的表达;Western Blot检测信号传导通路相关分子P38MAPK及P-P38MAPK的表达。结果:造模成功后,模型组近端结肠黏膜AQP3表达较空白组明显上升,而远端结肠黏膜AQP9表达较空白组明显下降(t值分别为3.148和7.069,P值均0.05)。肠润方联合P38MAPK抑制剂SB203580治疗后,近端结肠黏膜AQP3 m RNA水平显著上升(t=5.922,P0.05). The level of AQP3 m RNA in proximal colonic mucosa was significantly increased(t=5.922, P<0.01), and AQP9 m RNA was significantly decreased in distal colonic mucosa(t=4.038, P<0.01); The relative expression of P-P38/P38 protein was significantly decreased(t=19.419, P<0.01). Conclusion: The therapeutic action ofChangrun Formula on functional constipation rat models might through inhibiting the expression of AQP3 in proximal colon, and accelerating on expression of AQP9 in distal colon, and the regulating mechanism might relate with the inhibition on P38 MAPK and AKT phosphorylation.福建省自然科学基金项目(No.2012D043)~