Moleculor Clone and Functional Analysis of Betaine Aldehyde Dehydrogenase Gene of Avicennia marina

甜菜碱在植物体内以胆碱为底物,经两步酶催化反应合成,其中催化第二步反应的是甜菜碱醛脱氢酶(Betaine A l-dehyde Dehydrogenase,BADH).为研究甜菜碱醛脱氢酶基因在酵母中是否具有生物学功能,通过PCR-RACE技术从红树植物白骨壤(Avicennia marina)中分离得到BADH基因,将其转入酿酒酵母AH109中.通过对重组酵母生长曲线的测定,发现重组酵母对NaC l的耐受度由转化前的9%提高到14%,表明白骨壤BADH基因在酵母中能得到有效表达出具生物学活性的蛋白质.The synthesis of betaine in plants is two-step oxidation reaction of choline,and the the second step is catalyzed by betaine aldehyde dehydrogenase(BADH).In order to study the function of BADH gene in yeast,the BADH in Avicennia marina was obtained with PCR technique and transformed into Saccharomyces cerevisiae AH109.The salt tolerance of recombinant yeast increased from 9% to 14% by reorganizing the recombinant yeast′s growth curve.The results showed that the BADH gene can be effectively produce protein with biological activity in the recombinant yeast.国家自然科学基金(30771304);; 福建省自然科学基金(B0510002);; 国家基础科学人才培养基金项目(J0630649)资

Avirulent Strain of Ralstonia solanacearum Inducing Plant Systemic Acquired Resistance in Tomato

通讯作者: htzhou@ xmu.edu.cn; saiqunzh@163. com[中文文摘]青枯雷尔氏菌无致病力突变株RS-F523接种后能显著提高番茄对青枯病的抗性,但RS-F523在体外对强致病力菌株RS-F052没有直接的抑制作用.RS-F523接种后植株中与系统获得性抗性(systemic acquired resistance,SAR)相关的过氧化物酶(POD)活性、过氧化氢酶(CAT)活性,H2O2含量以及植株可溶性总蛋白都发生了波动变化,其变化方式和特点与RS-F052接种植株后的变化完全不同,提示RS-F523的生防机制可能与其接种后导致植物的SAR有关.[英文文摘]Tomato resistance ability to Ralstonia solanacearum RS-F052 improves after being inoculated with avirulent strain RS-F523,although RS-F523 cannot inhibit the growing of RS-F052 in vitro.The activities of POD and CAT,the amount of H2O2 and the soluble total proteins,which are all believed to associate with systemic acquired resistance,are tested in the plant which is inoculated with RS-F523.The results show that each of the indexes above fluctuates and differs from that of the plant inoculated with RS-F052,indicating that the biocon trolmechanism of RS-F523 is associated with SAR.国家自然科学基金(30771304);福建省自然科学基金(B0510002);福建省科技计划项目(2005N045);国家基础科学人才培养基金项目(J0630649)资

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^