基于生物质谱技术的磷酰化修饰策略在多肽测序中的应用

该文建立了一种利用磷酰化修饰结合电喷雾质谱(ESI-Q-TOF)测定多肽氨基酸序列的有效方法。利用Atherton-Todd反应,以二丙基亚磷酰酯(DPP)为磷酰化试剂,应用生物质谱技术,对磷酰化修饰后的5种模型肽的磷酰化反应情况进行了系统研究,考察了磷酰化肽的二级质谱特征,并与未经磷酰化反应的肽的二级质谱特征对比。结果表明,经过磷酰化修饰后,肽的二级质谱中的a1离子信号强度明显增加,可以准确鉴定其N端氨基酸;b系列离子信息完整,信号强度增强,使得多肽C ID测序的谱图简单、清晰,有利于肽的氨基酸序列的测定;赖氨酸(K,128.10 u)和谷氨酰胺(Q,128.13 u)两种氨基酸质荷比相近,由于二者磷酰化修饰后的差异性,使其得到准确区分。经过5种已知氨基酸序列的模型肽的磷酰化后结合质谱技术进行氨基酸序列测定验证,结果表明该方法简单、快速、准确,提高了利用质谱技术进行多肽测序的准确度和灵敏度,可为蛋白质组学研究提供有效的技术手段

杂色山雀产卵期回巢行为及节律研究

鸟类的繁殖行为和活动对整个种群的发展至关重要，是鸟类行为学研究的热点问题。人们在研究鸟类繁殖行为时大多关注育雏期，往往忽略了产卵期。鸟类产卵是高耗能的过程，研究鸟类产卵期回巢行为及节律，有助于更全面了解鸟类的繁殖策略。本文以杂色山雀（Sittiparus varius）为研究对象，在2019年3—7月对进入产卵期的繁殖巢箱进行监测，记录杂色山雀日回巢频率、停留时长以及衔巢材等情况。结果显示：（1）杂色山雀产卵时间为4：00—5：30，产卵期间有雌鸟夜宿巢箱现象，并观测到鸟类间 “情饲”行为。（2）产卵期大多数是只有雌性回巢，雄性极少回巢，双亲回巢次数差异极显著（P=0000，n=11）。（3）产卵期间雌性对其自身巢花费较多时间及能量投入，雌鸟在上午回巢较频繁，以补巢行为为主，雄性回巢既不补巢也不进入巢箱。（4）雌雄总回巢次数与产首枚卵日期呈显著负相关关系（P=0011，n=11）

Periodic Mesoporous Ethanesilicas with trans-(1R,2R)-Diaminocyclohexane in the Framework for Asymmetric Catalysis

Mesoprous ethane-silicas with trans-(1R,2R)-diamninocyclohexane bridged in the framework were synthesized by one-step co-condensation under basic/acid conditions. These novel materials (complexed with [Rh(cod)Cl]2) are efficient catalysts for the asymmetric transfer hydrogenation of acetophenone. Compared with pure mesoporous silica counterparts, these chiral ethane-silicas are twice more active, which could be contributed to the increased hydrophobicity of the mesoporous ethane-silicas. Tuning the mesophase of the material from MCM-41 to SBA-15 can further improve the catalytic activity of the resulting catalyst. Adjusting the linker of the chiral ligand from propyl group to benzyl group can boost the catalytic activity and chiral inductivity of the materials. Key words: Periodic Mesoporous Organosilicas, Chirality, trans-(1R,2R)-Diaminocyclohexan

Adaptation of leaf anatomical characteristics of eleven understory species to different environments in coniferous plantations

人工林内两种主要生境（林窗和林内）的光照条件存在着显著差异，而这种差异也是影响人工林林下植物叶片形态和结构的重要环境因子。对川西米亚罗林区60a人工云杉林2种不同生境（林窗和林内）的11种植物比叶重、叶片解剖结构及气孔特征等进行了比较研究，结果显示：林窗生境中11种供试植物比叶重（LMA）均显著大于林内生境；多数阔叶物种叶片厚度（LT）、栅栏组织厚度（PT）、栅栏组织厚度／海绵组织厚度（PT／ST）、栅栏细胞层数在林窗生境中更大或更多，但叶表皮细胞厚度（UET、LET）、海绵组织厚度（ST）在两种生境中无显著差异；林窗生境中生长的各物种栅栏细胞均为长形且长度大于林内生长的相同物种；不同物种气孔密度（SD）对两种生境有一致的响应趋势，但显著程度有异，除三褶脉紫菀外10种植物的气孔长径（SL）受两种生境影响不大，这与气孔特性受多种环境因素制约，且各种因素对气孔作用效应和作用方面不同有关。结合各物种喜光特性综合分析后认为同一群落的林下植物对光生境具有一定程度的趋同适应性；从可塑性方面分析发现叶片光合组织的可塑性大于非光合组织，且各物种叶片平均可塑性与喜光特性基本吻合

Research on Rotating Permanent Magnet Eddy Current Brake

This paper studied the non-friction rotating permanent magnet eddy current brake technique, which was used on nonpowered vehicles. Based on the theory of eddy current brake, the rotating permanent magnet eddy current brake was designed according to the structure of CRH2 EMU’s trailer bogie. The dynamic analyses of rotating permanent magnet eddy current brake was made by ANSYS,the brake’s power at different speeds was calculated. At different numbers of pole pairs, circumferential distances of the pole, thickness of pole pad and gap widths of rotating permanent magnet eddy current brake, the running resistances caused by leakage of magnet field were analyzed. The conclusions got from this article provide some theory foundations for application of the rotation eddy current brake