Physical health care monitoring for people with serious mental illness

Background Current guidance suggests that we should monitor the physical health of people with serious mental illness and there has been a significant financial investment over recent years to provide this. Objectives To assess the effectiveness of physical health monitoring as a means of reducing morbidity, mortality and reduction in quality of life in people with serious mental illness. Search methods We searched the Cochrane Schizophrenia Group Trials Register (October 2009) which is based on regular searches of CINAHL, EMBASE, MEDLINE and PsycINFO. Selection criteria All randomised or quasi-randomised clinical trials focusing on physical health monitoring versus standard care or comparing i) self monitoring vs monitoring by health care professional; ii) simple vs complex monitoring; iii) specific vs non-specific checks iv) once only vs regular checks or v) comparison of different guidance. Data collection and analysis The authors (GT, AC, SM) independently screened search results and identified three studies as possibly fulfilling the review's criteria. On examination, however, all three were subsequently excluded. Main results We did not identify any randomised trials which assessed the effectiveness of physical health monitoring in people with serious mental illness. Authors' conclusions There is no evidence from randomised trials to support current guidance and practice. Guidance and practice are based on expert consensus, clinical experience and good intentions rather than high quality evidenc

Parameter Distributions of Inositol 1,4,5-Trisphosphate Receptor Channel Model with the Immune Algorithm

肌醇1,4,5-磷酸三酯受体(InOSITOl 1,4,5-TrISPHOSPHATE rECEPTOr,IP3r)是内质网钙离子释放的重要通道,对细胞信号的传递至关重要。国内外已经提出一些IP3r通道模型,用来解释拟合IP3r通道膜片钳实验数据,其中的dE yOung-kEIzEr模型在数值模拟中应用最为广泛。随着人工免疫系统研究的迅速发展,人工免疫算法已经在多目标优化问题上取得了广泛应用。作者应用混合免疫多目标优化算法,根据爪蟾卵母细胞和草地夜蛾细胞核膜IP3r通道的开放概率、平均开放时间和平均闭合时间的动力学实验数据,对dE yOung-kEIzEr通道模型进行参数拟合,获得大量优化的拟合参数,从而得到通道模型参数分布图。通过对比两种细胞的拟合参数分布,指出这两种不同细胞IP3r通道的开关动力学差异,主要来源于抑制CA2+结合时IP3结合位点和当IP3结合时抑制CA2+结合位点的结合动力学不同。The inositol 1,4,5-trisphosphate receptor(IP3 R),which is a Ca2+-release channel in the endoplasmic reticulum,plays an important role in cellular signaling system.In recent years,several IP3 R models have been proposed to fit the stationary data of patch clamp recordings.The De Young-Keizer model is one of the most widely applied IP3 R models.With the rapid development of artificial immune system,the artificial immune algorithms have been widely applied to multi-objective optimization problems.In this paper,the authors applied the immune algorithm to fit the parameters in De Young-Keizer model according to the nuclear membrane experimental data of open probability,mean open time and mean closing time of the Xenopus oocyte and Spodoptera frugiperda Sf9 cells.By comparing the fitting parameter distributions of the two types of channels,it is indicated that the differences in the IP3 R channel dynamics between the two types of cells are originated from the dynamic differences in the IP3 binding site when inhibitory Ca2+is bonded and the inhibitory Ca2+binding site when IP3 is bonded.国家杰出青年科学基金项目(11125419); 福建省高校领军人才资助计划; 教育部新世纪优秀人才支持计划(NCET-12-0649)~

新型中心波长稳定的高效率L波段掺铒光纤超荧光光源

采用后向抽运的两级级联的掺铒光纤超荧光光源的新型结构,运用同步抽运技术对两级光纤按比例进行后向抽运,从而实现高效率且中心波长稳定的L波段超荧光光源输出。研究了光纤总长度、两级光纤长度分配以及抽运比例对超荧光光源输出特性参数的影响。结果表明,通过采用两级级联后向结构可以将光谱从C波段有效地转移到L波段,而且能够实现在高抽运功率下具有中心波长对抽运功率波动不敏感的特性。在290 mW功率1;1抽运下,实验获得了输出功率97 mW,线宽47.3nm,中心波长稳定的L波段超荧光光源

Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用

【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50 μmol/L)诱导各组细胞凋亡,行Hoechst33258细胞染色对比观察各组细胞凋亡情况,通过MTT法分析各组细胞存活率?RT-PCR与Western blotting检测转染后Bcl-xl基因的表达情况.【结果】 转染SH-SY5Y细胞后,检测结果显示Bcl-xl基因能够高效转染并在此细胞中蛋白表达，相对于未转染细胞来说,转染细胞能部分对抗硝普钠诱导的凋亡,MTT显示有统计学意义(P < 0.05).【结论】 Bcl-xl基因能够通过升高基因的表达抑制NO诱导的类神经元细胞死亡,同时也能间接说明硝普钠产生的NO对细胞的毒性可能大部分是通过凋亡途径来产生的

碳离子诱导的DNA双链断裂

ＤＮＡ双链断裂（ＤＳＢｓ）是电离辐射诱导的最重要的原发损伤，研究ＤＳＢｓ有利于揭示细胞辐射敏感性的机理。用倒转脉冲场凝胶电泳结合荧光扫描进行ＤＮＡ定量研究７５ＭｅＶ／ｕ１２Ｃ６＋对小鼠Ｂ１６黑色素瘤细胞ＤＳＢｓ的诱导，结果表明：ＤＳＢｓ产额约为０．７４ＤＳＢｓ／１００Ｍｂｐ／Ｇｙ；ＤＮＡ片段分布在两个区域。大片段区分子量约为１．４Ｍｂｐ～３．２Ｍｂｐ，分子量小于１．２Ｍｂｐ的为小片段区；并且随着剂量的增加，大片段区ＤＮＡ含量逐渐下降，而小片段区的ＤＮＡ含量显著增加。表明Ｂ１６ＤＮＡ分子上可能存在对重离子较为敏感的位点。攀登计划（B）”项目,中国科学院兰州分院基

General physical health advice for people with serious mental illness

There is currently much focus on provision of general physical health advice to people with serious mental illness and there has been increasing pressure for services to take responsibility for providing this. For the comparison of physical healthcare advice versus standard care we identified five studies (total n = 884) of limited quality. For measures of quality of life one trial found no difference (n = 54, 1 RCT, MD Lehman scale 0.00 CI -0.67 to 0.67) but another did (n = 407, 1 RCT, MD Quality of Life Medical Outcomes Scale - mental component 3.7 CI 1.7 to 5.6). There was no difference between groups for the outcome of death (n = 407, 1 RCT, RR 1.3 CI 0.3 to 6.0), for the outcome of uptake of ill-health prevention services, one study found percentages significantly greater in the advice group (n = 363, 1 RCT, MD 36.9 CI 33.1 to 40.7). Economic data were equivocal. Attrition was large (> 30%) but similar for both groups (n = 884, 5 RCTs, RR 1.18 CI 0.97 to 1.43). Comparisons of one type of physical healthcare advice with another were grossly underpowered and equivocal