DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

Dynamic Volatility Relationship between the CSI300 Futures and the Spot Market:Perspective from the VECM-GJR-DCC-MGARCH-t Model

在VECM-dCC-MgArCH模型基础上,以gJr形式考虑变量非对称作用、用T分布来描述股市数据的非正态分布特征,构建了VECM-gJr-dCC-MgArCH-T模型,并实证分析推出沪深300股指期货至今,沪深300期现货市场的动态波动关系。结果表明:沪深300期现货市场波动之间整体有较高关联性,但相关程度变化不定,在行情上涨时期两者关系大幅减弱;同时,现货市场波动对不利冲击的反应更敏感;现货市场过去意外冲击和过去波动都会抑制期货市场波动,而期货市场过去意外冲击和过去波动则会加剧现货市场波动。Based on the VECM-DCC-MGARCH model,considering asymmetric effect through GJR form and using t-distribution to describe abnormal distribution characteristics,we proposes an improved VECMGJR-DCC-MGARCH-t model,and empirically analyses dynamic volatility relationship between the CSI300 futures and the spot market since establishment of CSI300 index futures.The results show that there exists a high correlation between CSI300 futures and spot market volatility as a whole,but the correlation varies.In particular when the market quotation goes up,their correlation significantly reduces.Furthermen,the spot market's reactions to negative influence is more sensitive.Finally,past unexpected volatilities and past volatilities in the spot maket weaken the fluctuations of futures,though past unexpected fluctuations and past fluctuations in the futures strengthen the spot market volatilities.国家自然科学基金面上项目(71373219);国家自然科学基金青年项目(71103150); 全国统计科学研究计划项目(2012LY045); 中央高校基本科研业务费项目(2013221012); 江苏高校优势学科建设工程资助项目(PAPD); 江苏省普通高校研究生科研创新计划项目(CXLX12_0596

基于多孔吸滤材料的自动灌排垂直 绿化装置的结构设计和参数确定

为了实现垂直绿化中针对不同景观植物的灌溉需求，设计了基于多孔吸滤材料的自动灌排垂直绿化装 置。装置利用土壤水分平衡原理，通过多孔吸滤材料使土壤水势由负压向正压的变化，营造不同的土壤水分环境，以 满足多种景观植物需水要求，并通过土壤含水率与土壤基质势之间耦合关系调节灌水器出流量，实现针对不同植物 的自动灌溉。为了确定装置参数，将水箱高度、土壤埋深、吸水线材材质及直径、多孔陶瓷渗水片原料配比作为待定参 数，通过景观植物灌溉需水量的理论计算、不同线材材质及直径对土壤吸水高度和含水率的影响试验的结果分析，多 孔陶瓷渗水片原料配比优选，确定了装置中各项待定参数。通过效益分析对比，装置较现有垂直绿化装置成本降低 50% ～ 70%。</div

设计流量和土壤质地对微孔陶瓷灌水器入渗特性的影响

为探明微孔陶瓷灌水器土壤中入渗流量变化的原因，明确微孔陶瓷灌水器的出流原理，该研究基于土桶模拟试 验，研究3 种设计流量（0.72、1.87 和4.40 L/h）的微孔陶瓷灌水器下2 种土壤（黄绵土、塿土）的渗流特性。结果表明， 使用不同灌水器灌溉后，短时间内入渗流量均迅速减小，而后缓慢减小趋于稳定。设计流量与土壤质地均影响灌水器的 出流。灌水器周围土壤水势的变化是造成入渗流量变化的直接原因，土壤含水率的变化是入渗流量变化的根本原因。在 没有淹没出流的情况下，土壤含水率越高，入渗流量越小。设计流量为1.87 L/h 灌水器应用于塿土中，当土壤含水率由13%增大至40%时，入渗流量由1.4 L/h 下降至0.3 L/h 左右。灌水器周围土壤含水率对入渗流量具有反馈调节作用。采用 微孔陶瓷灌水器作为灌溉系统的核心部件，在内部水头适宜（微压或零压）的情况下，通过灌水器入渗流量与土壤含水 率的耦合作用，可实现土壤水分的自动调控，达到主动灌溉的目的。该文可为微孔陶瓷灌水器的推广应用提供参考。</p

The Cloning of complementary DNA encoding p49 ES antigen of Trichinella spiralis

应用dnA重组技术将编码旋毛虫肌幼虫49kdA抗原的基因克隆于大肠杆菌中.设计特异的PCr引物，用PT-PCr技术直接从旋毛虫肌幼虫总rnA中反转录并扩增出约1.1kb的靶dnA.用bAMHI和ECOrI酶解后将其克隆到质粒载体PuC19中，用X-gAl培养基筛选重组子.该重组子经相同的核酸内切酶水解获得约2.7kb和1.1kb两个片段，分别与PuC19和目的基因的大小相同，目的基因经序列分析并与文献报道的序列比较发现具有97.8%的同源性.Complementary DNA(cDNA) encoding 49KD ES antigen of Trichinella spiralis muscle larvae was cloned in E.coli by recombinant DNA technology.Aabout1.1kb DNA fragment was obtained by RT PCR from total RNA of Trichinella spiralis using a specific 5′ end 3′ end primer.PCR product was cleaved with restriction enzymes EcoRI and BamHI and ligated into the pUC19 vector.The cloning was screened by X gal medium.When the recombinant DNA was cleaved by the same enzynes, 1.1kb and 2.7kb fragments were obtained, which were similar to the langth of PCR product and of pUC19 vcvtor.There is 97.8 percent homologous between the sequence of PCR product and the sequence which reported in literature by sequencing analysis.福建省重点（农医）资

诱导小鼠骨髓间充质干细胞向雄性生殖细胞的定向分化

目的 探讨小鼠骨髓间充质干细胞是否能够在体外被诱导发生向雄性生殖细胞方向的分化.方法 从雄性小鼠骨髓中分离能够长期贴壁生长的细胞,并鉴定其是否为间充质干细胞.对分离的细胞进行生殖细胞特异性报告基因标记(stra-8-GFP).采用视黄酸诱导标记的细胞发生向生殖细胞方向的分化.通过观察报告基因表达和生殖细胞相关基因mRNA表达情况确定是否发生了分化.结果 从小鼠骨髓中分离到的贴壁生长的细胞表达间充质干细胞的表面标志CD90、CD44、CD105和Sca-1;细胞在体外可以被诱导分化为成骨、成软骨及成脂肪细胞.报告基因标记的间充质干细胞在被视黄酸诱导2d后开始表达绿色荧光蛋白和生殖细胞相关基因Mvh、Fragilis和Stella的mRNA.未经视黄酸诱导的细胞不表达绿色荧光蛋白和生殖细胞相关基因.结论 小鼠骨髓间充质干细胞在体外可以被视黄酸诱导发生向雄性生殖细胞方向的分化. Objective To investigate if mouse bone marrow derived-mesenchymal stem cell can be differentiated into male germ cell in vitro. Methods Mesenchymal stem cells were isolated from mouse bone marrow and were identified by surface markers and differentiation study in vitro. Germ cell specific reporter gene (stra-8-GFP) was transfected into the identified cells with Lipofectin 2000; Retinoic acid was used to induce the transfected cells to differentiate into germ cell. The germ cell differentiation was evaluated by expression of reporter gene and germ cell specific genes. Results The isolated cells had positive expression of CD90, CD44, CD105, Sca-1, similar with mesenchymal stem cells, and could be differentiated into osteocytes, chondrocytes and adipocytes in vitro. After being induced by retinoic acid for two days, the expression of GFP and germ cell specific gene mRNA were detected in the stra-8-GFP transfected cells. Conclusion Mouse bone marrow-derived mesenchymal stem cells could be differentiated into germ cells in vitro under induction of retinoic acid

Lin28调节小鼠胚胎干细胞向原始生殖细胞分化

目的探讨小鼠胚胎干细胞（mouse embryonic stem cells，mESCs）向原始生殖细胞（primordial germcells，PGCs）分化过程中特异基因表达变化及可能机制。方法mESCs分化形成拟胚体（embryoid bodies，EBS），不同浓度atRA（1uM，2uM，5uM）持续诱导EBS16h、2d和5d，RT—PCR、和免疫荧光分别检测Lin28和Blimpl以及相应蛋白的表达变化。结果atRA诱导16h的EBs其Lin28mRNA表达量较高，随着诱导时间延长而逐渐降低，Blimpl则无明显变化。WB结果显示EBs的Lin28蛋白表达随诱导时间延长逐渐减弱，而Blimpl蛋白表达逐渐增强。免疫荧光显示Lin28和Blimpl阳性信号均定位于细胞胞浆，其变化特点与WB结果相一致。结论EBs经atRA诱导后可影响Lin28的表达，Lin28和Blimpl的变化并不协调，其蛋白表达随着诱导会随之发生变化。atRA诱导可能使PGCs分化的Lin28低水平表达时期提前出现

化学法MgCl_2——n-BuOH——SiCl_4——TiCl_4体系丙烯等规聚合催化剂中n-Bu含量对催化行为的影响

用丁醇和氯化镁制成醇溶剂化物,经SiCl_4解醇而得到的MgCl_2晶体,其中包含有少量的硅酯并吸附有丁醇。丁氧基的存在对以后制成的催化剂的性能有很大的影响。将TiCl_4负载于上述反应法制备的MgCl_2载体上,TiCl_4便与载体上残存的丁醇及表面的硅酯作用生成烷氧基氯化钛。改变制备条件及负载不同配比的氯化钛-丁氧基钛混合物,便可得到烷氧基含量不同的催化剂。催化剂前身的表面上钛化合物可用Ti(n-BuO)nCl_4-n(n<4)表示。本实验结果表明,若n大于0.1,随着n值的增大,催化剂的活性下降,产物的等规度也偏低,而聚合物的分子量则升高。本文对这些规律作了些探索性的解释,为改进这种催化剂提供一定线索

内蒙古典型草原植物叶片碳氮磷化学计量特征的季节动态

分析植物叶片(C)、氮(N)、磷(P)含量及其比值的季节动态,不仅有助于认识植物生长发育和养分吸收利用等生理生态过程,也有利于认识植物化学计量的动态平衡关系。该文选择内蒙古典型温带草原18种常见植物,在生长季的6–9月,每半月一次进行连续采样,在此基础上分析了叶片C、N、P含量及其比值在生长季内的变化。主要结果:1)植物叶片C、N、P含量及其比值的季节性变化在不同功能类群间不同步,其中叶片N、P含量的季节变化体现了明显的稀释作用。2)叶片C、N、P含量及其比值在不同功能类群间差异显著,单子叶、多年生禾草类的叶片N、P含量显著低于双子叶和多年生杂类草植物,而其叶片C:N、C:P则高于双子叶和多年生杂类草植物。3)叶片N、P含量显著正相关,叶片C:N和C:P分别与N和P含量显著负相关,可能体现了植物体内营养元素间的内在耦合机制。4)叶片N含量与C:N,叶片P含量与C:P以及叶片N含量与P含量均呈现等速生长关系,且等速生长关系在生长季保持稳定