A Comparative Study on the Extration of β-carotene and its Content of the Ten Species of Dunaliella (Chlorophyceae)

建立了从杜氏藻鲜藻中直接提取、纯化β-胡萝卜素及快速检测其含量的新方法，发现在十种（株）杜氏藻dunAlIEllAbArdAuIl中生成β-胡萝卜素的量最高，达藻干重的4.19%，在最佳条件下可达13%，经动物急性毒性实验测得小鼠对杜氏藻β-胡萝卜素的最大耐受量＞240Mg·kg￣（-1）.A new method has been used For extraction,puriFication and determination of thecontent level of β-carotene From the green alga Dunaliella.It has been Found that D .bardauil can accu-mulate 4.19%β-carotene of its dried weight.The content of β-carotene is the highest level in the tenspecies(strains) of Dunaliella tested.Morever under a Favourable condition,D.bardauil is able to pro-duce β-carotene as much as 13% of its dried weight.According to the tolerance determination,thetolerance For rats to the β-carotene extracted From Dunaliella was more than 240 mg·kg￣(-1)