Isolation, Biological Activity and Secondary Metabolite Investigations of Marine Actinomycetes

天然产物是药物的重要来源。海洋具有高压、低温、高盐度等极端环境，生活在其中的海洋放线菌已发展出独特的代谢方式，这不仅确保这类微生物能够在极端环境中生存，也造就了其产生新颖的次级代谢产物的能力。 本论文采用分散差速离心法与4种选择性分离培养基，从15份台湾海峡海泥样品中分离到海洋放线菌216株，其中脯氨酸-MOPS培养基与聚乳酸培养基对于海洋放线菌有良好的分离效果。 采用固体发酵与液体发酵方式，对216株海洋放线菌进行小量发酵，获得432份发酵初提物。以6株细菌和2株真菌作为指示菌，对432份发酵初提物进行抗菌活性筛选，有164株(77%)海洋放线菌对8种指示菌中的一种或几种具有抑制作用，有...The nature products are important source of the medicine. As marine environmental with high-pressure, low temperature and high salinity, marine actinomycetes have developed unique metabolic way, it is not only ensuring its survival in marine, but also provides the potential of producing novel metabolites. In this thesis, DDC (dispersion and differential centrifugation) method and four isolation m...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2172009115212

Ru修饰Pd二十四面体纳米晶的合成及其甲醇电催化氧化性能

表面结构控制和表面异种金属修饰是调控催化剂反应性的重要方法。因此,我们结合高指数晶面结构的高反应性与表面修饰异种金属,合成具有{730}高指数晶面的钯二十四面体纳米晶,并通过循环伏安扫描电沉积法得到Ru修饰的钯二十四面体纳米晶。电化学测试结果表明,低的Ru覆盖度(θRu=0.08)可显著提高对碱性介质中甲醇电氧化的催化性能。电化学原位红外光谱结果表明,少量Ru的修饰没有减少CO的生成,而是促进了低电位下甲醇氧化成甲酸根。国家自然科学基金(21573183);;\n中央高校基本科研业务费(20720160045)资助项目~

Diversity and bioactivities of marine actinobacteria from marine sediments of Fujian coast

目的探索福建沿海沉积物中可培养海洋放线菌的多样性及其抗菌、抗肿瘤活性,为发现新的药物先导化合物提供新菌源。方法从15份福建沿海沉积物选择性分离放; 线菌;16S; rRNA基因序列分析多样性;采用10种活性筛选模型分析生物活性。结果共分离到216株海洋放线菌,MOPS-脯氨酸培养基的分离效果最好。选取77株; 代表菌株进行16S; rRNA基因序列分析,发现其归属于14个属,其中10株为潜在新种。有164株(77%)海洋放线菌对8种指示菌中的一种或几种具有抑制作用;有39株; (18.1%)对HeLa细胞或HepG2细胞有细胞毒活性(抑制率≥50%)。结论福建沿海沉积物样品中含有丰富多样的海洋放线菌,大量菌株具有抗菌与; 抗肿瘤活性,是天然产物开发的重要资源。Objective To find new lead compounds, the diversity, antimicrobial and; antitumor activities of marine actinobacteria from marine sediments of; Fujian coast were investigated. Methods Actinobacteria strains were; selective isolated from 15 marine sediments of Fujian coast. The 16S; rRNA gene sequences analysis of representative strains were carried out; to evaluate their diversity. All of the isolates were assessed using 10; screening models for their bioactivities. Results A total of 216 marine; actinobacteria were isolated by using four media. The isolation effect; of MOPS-proline medium was the best choice. Phylogenetic analysis based; on 16S rRNA gene sequences showed that 77 representative strains; belonged to 14 genera, and there were 10 strains identified as potential; novel species. One hundred and sixty-four (77%) strains showed; antimicrobial activity against at least one kind of microorganisms.; Thirty-nine (18.1%) strains showed cytotoxic activity with inhibition; rate above 50% against Hela or HepG2 cells in vitro. Conclusion This; study indicated that actinobacteria from marine sediments of Fujian; coast are abundant, many of them had antimicrobial and cytotoxic; activities, which can be further explored.厦门市科技计划项

Electrochemical Characterization of (PhMgCl)2-AlCl3/Mixed Ether Electrolytes

制备了全苯基有机铝镁盐(PhMgCl)2-AlCl3，研究以3种不同混合醚，即dimethoxyethane (DME)+THF、Diglyme (DG)+THF和Tetraglyme (TG)+THF作溶剂对全苯基有机铝镁盐在不同金属电极上的电化学性能的影响. 结果表明，和(PhMgCl)2-AlCl3/THF体系相比，(PhMgCl)2-AlCl3/DG+THF(3:2)仍具有较高的离子电导率（1.605×10-3 S?cm-1）、良好的可逆沉积镁特性及阳极抗氧化性能（电化学窗口大于2.8 V）. 且该DG+THF混合溶剂还可大幅降低电解液的饱和蒸汽压（由23.46 kPa降低到9.41 kPa），减少了电池使用过程电解液的挥发，从而提高了可充镁电池的安全性能. 比较Pt、Ni、Cu和Al等不同金属基质电极，发现Pt的电化学性能最好，而Al最差.The (PhMgCl)2-AlCl3 salts were prepared and the electrochemical properties of (PhMgCl)2-AlCl3 electrolytes containing dimethoxyethane(DME)+THF, Diglyme(DG)+THF and Tetraglyme(TG)+THF solvents with different proportions on various metal electrodes were studied. Compared with (PhMgCl)2-AlCl3/THF, the (PhMgCl)2-AlCl3/DG+THF(3:2) solvents showed high ionic conductivity of 1.605×10-3 S?cm-1, good reversible Mg deposition behavior and anodic anti-oxidation performance with an electrochemical window wider than 2.8 V. In addition, the DG+THF mixed solvent could greatly lower the saturated vapor pressure by reducing from 23.46 kPa to 9.41 kPa, which suppresses volatilization of the electrolyte and improves the overall safety of rechargeable battery. Among Pt, Ni, Cu and Al electrodes, Pt offers the best electrochemical properties, while Al the worst.国家重点基础研究发展计划973课题（2007CB209700）资助作者联系地址：上海交通大学化学化工学院，上海 200240Author's Address: School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China通讯作者E-mail：[email protected]

Application of Ionic Liquid PP14TFSI in Electrolyte Systems for Rechargeable Mg Batteries

制备了可充镁电池电解质苯酚基镁盐，以四氢呋喃（THF）与N-甲基-N-丁基-哌啶-双三氟甲基磺酰胺（PP14TFSI）离子液体混合物代替四氢呋喃作为该电解质的溶剂. 当THF与PP14TFSI体积配比为1:1时，该苯酚基镁盐电解液镁可逆溶出性能最佳，电化学窗口宽（2.7 V vs. Mg），离子电导率高（7.77 mS·cm-1）. 此外，热重测试表明离子液体的加入大大降低了THF溶剂的挥发性，提高了可充镁电池的安全性能. 四氢呋喃 + N-甲基-N-丁基-哌啶-双三氟甲基磺酰胺混合溶剂有望作为可充镁电池电解液的首选溶剂.A phenolated-based Mg salt was prepared. The mixture of THF and N-methyl-N-butyl-piperidinium bis(trifluoromethanesulfonyl)imide (PP14TFSI) serves as the mixed solvent instead of pure THF for rechargeable Mg batteries systems. This new type of electrolyte system not only has optimal reversibility of Mg, but also owns a wide electrochemical window (2.7 V vs. Mg) and high ionic conductivity (7.77 mS·cm-1). More importantly, the addition of Ionic Liquid suppresses the volatility of THF and improves the safety of rechargeable Mg batteries. The result shows that the mixture of THF and piperidyl-based Ionic Liquid could be regarded as a good solvent candidate for rechargeable Mg batteries.作者联系地址：上海交通大学化学化工学院，平野材料创新研究所，上海 200240Author's Address: School of Chemistry and Chemical Engineering, Hirano Institute for Materials Innovation, Shanghai Jiao Tong University, Shanghai 200240, China通讯作者E-mail：[email protected]