The Research and Application of Algorithm of Syllabus by ‘Walking in the Mine Fields’

二十世纪五十年代，国外就有人着手研究用计算机来编制课表，1975年Even等人证明了课表问题属于NP完全问题，是典型的组合优化和不确定性调度问题，时间和空间作为临界资源的一种不定型分配，这是一类困难问题。但是这类问题有它固有的数学模型，对于这类问题，人们至今还没有得到其通用的算法，而是更倾向于去寻找一种有效的近似算法。一个可行的课表满足以下条件：⑴同一时间，一个教师只能上一门课程；⑵同一时间，一个学生只能上一门课程；⑶同一时间，一个教室只能上一门课程；国内外许多专家和学者从不同的侧重点设计出各种排课算法，其中不凡优秀。但大多算法有其不足之处，存在局限性。经过反复认真和冷静的思考，‘雷区行走’排...Since the 1950’s,some people have got down to researching making school timetable with the help of computer. In 1975,Even and other proved that the aspect of school timetable belongs to NP complete problems, which is the typical combination and indefinite assignment, and which is regarded as the indefinite resource arrangement in time and space. It is indeed a difficult problem. However, it has a ...学位：工学硕士院系专业：信息科学与技术学院计算机科学系_计算机应用技术学号：X20034305

Preparation of Nano-pore Insulation Panels Based on Fumed Silica

本文以纳米多孔SiO2粉末和两种玻璃纤维作为原料采用湿法工艺制备不同系列的隔热板，并选择γ-氨丙基三乙氧基硅烷(KH-550)、γ-（甲基丙烯酰氧）丙基三甲氧基硅烷(KH-570)作为表面修饰剂，对所制备的纳米孔隔热板进行表面修饰改性研究。 本文主要研究了制备工艺对试样成型的影响、纳米多孔SiO2粉末和玻璃纤维质量比与材料机械性能之间的关系、SiO2粉末和玻璃纤维质量比与材料隔热性能之间的关系、不同直径的玻纤对材料隔热性能的影响，还研究了表面修饰剂种类与溶液浓度、溶液pH值、修饰时间、修饰温度等因素对试样表面疏水性能的影响。研究发现： 1.制备工艺对试样成型效果影响较大。研究结果表明，制备...In this paper, a series of different mass ratio thermal insulation boards based on fumed silica powder and two kinds of glass fibers was prepared. The boards were modified with γ-Aminopropyl triethoxysilane (KH-550) and γ-Methacryloxypropyl trimethoxysilane (KH-570), respectively. The effects of preparing technology on specimen molding were studied. The optimal preparation parameters were determi...学位：工程硕士院系专业：材料学院_材料工程学号：2072010115005

EQUATION ON COMPLEX MANIFOLDS

本文得到了复流形上边界不一定光滑的强拟凸域上的kOPPElMAn-lErAy公式，并得到这个域上的-方程的解的积分表示，这个表示的特点是不含边界的积分The author obtains the Koppelman Leray Formula For a strictly pseudoconvex domain with not necessarily smooth boundary in complex maniFolds, and an integral representation For the solution of equation on this domain is obtained

C~n中(p,q)型微分形式的-闭开拓

文中研究对给定在C~n中一域的边界上的C~(2)类(p,q)型微分形式越过边界的?闭开拓问题,得到了可以?闭开拓的二个充分必要条件和 Hartogs 与Bochner 型定理

Comparison of genomic DNA of hybrid ( Oreochromis niloticus ♀ × O.aureaus ♂) and its parents

采用RAPD技术 ,用 2 0个随机引物对尼罗罗非鱼、奥利亚罗非鱼及其杂交一代的精巢DNA进行扩增反应 ,发现引物OPZ - 14和OPZ - 18在尼罗罗非鱼和奥利亚罗非鱼之间扩增出有差异的DNA片段 ,作为鉴定两种鱼的分子标记有较高可信度。对这两种鱼的杂交一代基因组DNA的RAPD扩增能获得足够的多态性 ,2 0个引物中有 8个引物扩增出差异性产物 ,表明杂交一代基因杂合性增强 ,这是杂种优势得以形成的重要遗传物质基础之一。Using Random Amplified Polymorphic DNA(RAPD) technique with 20 kinds of random primers, genomic DNA of hybrid (Oreochromis niloticus ♀ ×Oreochromis aureaus ♂) and its parents were analysed. The results showed that only with the two kinds of primers, OPZ-14 and OPZ-18, species specific fragments were produced between parent fishes, even between different fish belonging to the same species, and some of them could be used as molecular markers to identify O.niloticus and O.aureaus. And 8 primers generate polymorphic products among hybrid fish and its parents. DNA fragments amplified from the hybrid fish were more abundant than those from its parents, which may be the result of more abundant genes in the hybrid fish, because of heredity or mutation. These results provide some molecular biological basis for species identification and heterosis effect in fish.福建省水产厅资助! (闽水外经 96 6 4

BOCHNER TECHNIQUE ON STRONG KHLER-FINSLER MANIFOLDS

By using the Chern-Finsler connection and complex Finsler metric,the Bochner technique on strong Khler-Finsler manifolds is studied.For a strong Khler-Finsler manifold M,the authors first prove that there exists a system of local coordinate which is normalized at a point v ∈ M-=T 1,0M\o(M),and then the horizontal Laplace operator H for diffierential forms on PTM is defined by the horizontal part of the Chern-Finsler connection and its curvature tensor,and the horizontal Laplace operator H on holomorphic vector bundle over PTM is also defined.Finally,we get a Bochner vanishing theorem for diffierential forms on PTM.Moreover,the Bochner vanishing theorem on a holomorphic line bundle over PTM is also obtaine

高职院校学风影响因素调研

学风建设是高职院校学生工作的重点,影响着高职院校的人才培养质量,也影响着高职院校的声誉和竞争力。随着我国高等教育的发展,高职院校学生群体特征不断变化,给高职院校学风建设带来了新问题。及时了解学校学风状况,探究影响高职院校学风的因素,深刻研究学生的学习目的和学习动机等方面存在的问题,进而采取有效措施推动学风建设,成为高

甲硫氨酸亚砜还原酶A在肾透明细胞癌侵袭转移中的作用机制

目的本研究旨通过细胞学和动物实验验证甲硫氨酸亚砜还原酶A（MSRA）对肾透明细胞癌（ccRCC）增殖、凋亡、侵袭和转移的影响，揭示其潜在的生物学机制，为ccRCC的治疗提供新的分子靶点和策略。方法利用转染实验构建MSRA过表达细胞株；通过细胞增殖实验、平板克隆实验、凋亡实验、划痕实验、侵袭实验评估MSRA对ccRCC细胞增殖、凋亡、侵袭和转移的影响；并进一步通过ROS检测，RT-qPCR、Western blot实验探讨其作用机制及相关信号通路；随后，利用裸鼠皮下移植瘤模型在动物水平上验证MSRA对ccRCC肿瘤生长和转移的影响，并初步探索其潜在的分子机制。结果OS-RC-2和786-O细胞株MSRA mRNA水平和蛋白表达量较低（RT-qPCR：0.57±0.09，0.56±0.04；WB：0.26 ±0.13，0.24±0.09）；RT-qPCR和Western blot实验提示成功构建了OS-RC-2-pLVSO2-MSRA（RT-qPCR：108.04±1.80；WB：117.01±20.19）和786-O-pLVSO2-MSRA（973.45±51.37，WB：190.34±30.13）过表达细胞株，差异具有统计学意义（P＜0.001）；增殖实验提示OS-RC-2-pLVSO2-MSRA（72 h：1.246±0.003）和786-O-pLVSO2-MSRA（72 h：1.468±0.001）增殖活力降低，差异具有统计学意义（P＜0.001）；平板克隆实验提示OS-RC-2-pLVSO2-MSRA（0.090±0.002）和786-O-pLVSO2-MSRA（0.080±0.002）克隆数减少，差异具有统计学意义（P＜0.001）；凋亡实验提示OS-RC-2-pLVSO2-MSRA（2.013±0.116）和786-O-pLVSO2-MSRA（4.767±0.199）凋亡率增加，差异具有统计学意义（P＜0.001）；划痕实验提示OS-RC-2-pLVSO2-MSRA（0.643±0.028）和786-O-pLVSO2-MSRA（0.603±0.034）迁移距离较少，差异具有统计学意义（P＜0.001）；Transwell实验提示OS-RC-2-pLVSO2-MSRA（16.80 ±2.28）和786-O-pLVSO2-MSRA（21.40±4.78）穿透细胞数减少，差异具有统计学意义（P＜0.001）；荧光实验显示OS-RC-2-pLVSO2-MSRA（50.59±6.24）和786-O-pLVSO2-MSRA（62.87±5.35）活性氧含量降低，差异具有统计学意义（P＜0.001）；Western blot实验显示OS-RC-2-pLVSO2-MSRA和786-O-pLVSO2-MSRA中N-cadherin和Vimentin的表达量下降，而E-cadherin的表达量增加，差异具有统计学意义（P＜0.001）；Western blot实验显示OS-RC-2-pLVSO2-MSRA和786-O-pLVSO2-MSRA中p-ERK1/2和p-SMAD3的表达量下降，差异具有统计学意义（P＜0.001）；动物实验提示OS-RC-2-pLVSO2-MSRA肿瘤体积降低（155.00±50.46），差异具有统计学意义（P＜0.001）；动物组织Western blot实验提示N-cadherin和Vimentin的表达量下降，而E-cadherin的表达量增加，差异具有统计学意义（P＜0.001）；OS-RC-2-pLVSO2-MSRA中IHC实验提示Ki-67、N-cadherin和Vimentin的表达量下降，而E-cadherin的表达量增加，差异具有统计学意义（P＜0.001）。结论表达MSRA抑制ccRCCROS的表达，抑制EMT过程，进而抑制ccRCC的增殖、侵袭和转移

Breeding high-yield oil-producing strain to use cheap carbon source by UV induced protoplast mutagenesis

【目的】研究并建立利用原生质体紫外诱变技术选育可利用廉价碳源发酵的高产油新菌株的方法。【方法】采用1.5%蜗牛酶和1.0%纤维素酶混合液水解去除细胞壁得到2A00015(近平滑假丝酵母,CAndIdA PArAPSIlOSIS)的原生质体,将其放于紫外灯下诱变及再生壁培养,筛选获得可利用廉价碳源发酵的高产油酵母,并采用气相色谱质谱联用法(gC-MS)测定其脂肪酸组成。【结果】突变效果最好的突变菌株2A00015/25用葡萄糖发酵培养7 d后,其生物量、油脂产率和产油量分别为17.77 g/l、58.12%和10.32 g/l,较原始菌株分别提高了12.45%、23.32%和38.68%;利用废糖蜜发酵培养,其生物量、油脂产率和产油量分别为18.54 g/l、49.44%和9.17 g/l,较原始菌株分别提高了9.09%、21.16%和32.18%。利用废糖蜜培养其产油效率虽低于利用葡萄糖培养,但从环境保护及原材料成本的角度考虑,用废糖蜜作为碳源发酵培养产生油脂更具优势。诱变菌株利用废糖蜜发酵后产生油脂经检测含有8种脂肪酸,其脂肪酸组成与植物油近似,其中不饱和脂肪酸含量占脂肪酸总量的82.4%。【结论】通过利用原生质体紫外诱变技术,成功选育出一株新的可利用廉价碳源的高产油海洋菌株,产油率达到49.4%,提高了21.2%。[Objective] We used UV induced protoplast mutagenesis to study breed a new high-yielding lipid-producing strain which could use cheap carbon source.[Methods] Get the 1.5% glusulase and 1.0% cellulose solution.Hydrolyze to remove the cell wall and obtain the protoplast of 2A00015(Candida parapsilosis).Put it under the ultraviolet lamp for mutagenesis and cultivate regenrated wall.Then screen to get the high oil generated yeast which could be fermented by low-cost carbon source.Determine its fat acid components by gas chromatography with mass spectrometric(GC-MS).[Results] Cultivated the mutant strain with best mutation 2A00015/25 in the glucose.We found that the biomass, oil yield rate and oil production are separately 17.77 g/L, 58.12% and 10.32 g/L, which are separately 12.45%, 23.32% and 38.68% higher than the original strain.We have also cultivated the mutant strain in the waste molasses and found the biomass, oil yield rate and oil production are separately 18.54 g/L, 49.44% and 9.17 g/L, which are separately 9.09%, 21.16% and 32.18% higher than the original strain.The oil yield rate is lower in the waste molasses cultivation than that in glucose cultivation.However, in consideration of environment protection and cost of raw materials, the waste molasses is of much more advantages.It is tested that the fat generated from the waste molasses fermentation consists of eight kinds of fat acid.Its fat acid components are similar to the vegetable oil, in which the content of unsaturated fatty acid comprised 82.4% of the total fatty acid.[Conclusion] Basing on UV induced protoplast mutagenesis, we have successfully bred a new high-yielding oil strain which can make use of the low-cost carbon source, with its oil production rate 49.4% which has increased by 21.2%.深海(微)生物资源勘探与资源潜力评价项目(No.DY125-15-R-01