Ecological Studies on the Nanoflagellates in Sanggou Bay

桑沟湾是我国北方重要的海水养殖海湾。从湾顶到湾口分别是贝类养殖区和藻类养殖区。本研究选择桑沟湾作为研究区域对养殖海区微型鞭毛虫的生态学进行研究。首先，于2011年春季，2011年夏季，2011年秋季和2012年冬季分别对桑沟湾微型鞭毛虫的时空分布、粒径结构和营养结构进行调查。而后，通过围隔实验研究贝、藻养殖对微型鞭毛虫的影响。根据围隔实验的结果设置营养盐添加实验和贝类摄食实验以探讨围隔实验中贝类增强微型鞭毛虫及其相关环节作用的原因。同时，本研究还探讨了微型鞭毛虫对微微型浮游生物的摄食。主要结果如下： （1）桑沟湾微型鞭毛虫以2-5μm微型鞭毛虫为主，微型鞭毛虫的总丰度的年变化范围从0.31×...Sanggou Bay in northern China is an important mariculture bay. The bay could be divided into the bivalves culture areas (B-area) and the macroalgae culture areas (M-area) from the bay head to the bay mouth. The ecological studies on the nanoflagellates (NF) in Sanggou Bay were conducted in the present study. Firstly, the spatial and temporal distributions, the size structure and the trophic struct...学位：理学博士院系专业：海洋与环境学院_海洋生物学学号：2242010015359

Research on facial spectrum and color characteristics of five-zang disease status

目的:观察疾病状态人群面色光谱色度情况,为疾病状态五脏病评价提供可供依据。方法:采用日产柯尼卡美能达CM-2600d分光测色仪,观测健康组(183例)、疾病组(370例)额部、眉间部、鼻部、下颏、左右颧部、左右眼胞8处明亮度l、红光度A、黄光度b、饱和度C值及波长段(400~700nM)下的面色反射率值等指标,并据中医理论对疾病状态进行五脏病位分析。结果:疾病组l值显著低于健康组,b、C值均显著高于健康组;疾病组各波长段下反射率显著低于健康状态组(P<0.05)。疾病组除左右眼胞外的6个位点间的l、A、b、C值有显著性差异(P<0.05),不同点位有其特异性的色度变化特征。疾病组五脏病各组光谱色度特征比较有显著差异(P<0.05),结果与中医面部脏腑五色理论在一定程度上相符合。结论:运用光谱色度测定方法,可以作为诊断疾病状态五脏病的重要指标。Objective: To observe disease status facial spectrum and color,providing good quantitative basis for disease assessment.Methods: We gathered the facial color information in health(183) and disease groups(370) including L,a,b,C values and reflection of different wavelengths in 400-700nm with CM-2600D spectral photometric color measuring instrument on 8 points including frontal part,glabellas nose,mandible two cheeks and eye cells and analysized disease status five-zang positioning according to the principle of TCM syndrome differentiation.Results: L value of Disease group was lower than health status group and b,C values were higher than health status group,reflection of different wavelengths of disease group were lower than health group(P<0.05).6 points in disease group between loci L,a,b,C values with varying degrees of significant differences(P<0.05).Different point had its own specificity facial complexion variation characteristics.Facial spectrum and color of each five-zang disease group had significant difference through comparion of facial complexion.To a certain extent the result was consistent with TCM facial organs Five-color theory.Conclusion: There existed diagnostic value in distinguishing five-zang disease status in some degree by spectral photometric color measuring technique.国家科技支撑计划(No.2012BAI37B06);国家高技术研究发展计划(863计划)资助项目(No.2008AA02Z407);国家自然科学基金资助项目(No.30873463;No.81173200);国家自然基金青年项目(No.81102558);上海市重点学科(第三期)建设项目(No.S30302;No.S30303)---

细粒棘球蚴细胞系培育及其免疫研究

从细粒棘球蚴病人体内获取生发层和原头节作为培养材料, 采用改良DMEM 培养液和鼠胶原蛋白包被的培养 瓶进行细胞培养, 建立了适合人源细粒棘球蚴细胞体外培养的方法, 并探讨棘球蚴细胞培养的制约因素。首次成功培育出一 株人源细粒棘球蚴细胞系(13G-5), 至1997 年3 月1 日为止该细胞系已培养了140 d, 其间传了21 代。该细胞系主要以成纤 维型细胞为主, 呈贴壁生长;用该细胞系细胞接种于昆明(Km)小鼠腹腔内, 能形成具包囊样结构的类似包囊;接种鼠产生对 囊液抗原的特异性抗体;ELI SA 能够检测出细胞系细胞代谢物中特异性抗原;该细胞系细胞和E .granulosus 原头节、包囊液 具有相同的ES T 酶带;使用该细胞系细胞的代谢物作为粗制抗原进行免疫预防接种, 能使60%的Km 小鼠获得完全抵抗E . granulosus 感染的能力;该抗原能使43.33%～ 77.42 %的包虫病人诊断出来。此外, 检定了人源细粒棘球蚴染色体, 染色体数 为14 ～ 18 条, 并首次对其进行了G-、C-带分析

Progress in the study of the selective feeding of heterotrophic nanoflagellate

微型异养鞭毛虫(Hnf)是海洋微微型浮游生物的重要摄食者,通过摄食作用对后者的种类(或类群)组成、粒径分布、数量结构和营养价值等属性具有重要的影响,而这与Hnf的摄食选择性有直接关系。对Hnf摄食选择性的研究有助于深入了解Hnf在海洋微食物环乃至整个海洋生态系统中的作用。就国际上已开展的Hnf摄食选择性相关研究进行了回顾,分析和总结了影响Hnf选择性摄食的关键因素,如食物大小、游动性、营养价值及食物细胞表面的生化结构特征等,并重点介绍了Hnf摄食选择性形成的主要机制以及Hnf的选择性摄食在调节海洋微微型浮游生物群落结构中的作用。Heterotrophic nanoflagellate(abbr.,HNF) is the principal consumer of picoplankton in marine ecosystem.Recognizing its selective feeding impact is essential to evaluate the role of HNF in the marine microbial food web.Over the last few decades,there has been a concerted effort by many researchers to the understanding of selective grazing behaviors among HNF.This paper,(1) summarize current knowledge on factors affecting HNF selective feeding;(2) discuss mechanisms involved in HNF selective feeding;(3) review the influence of HNF selective feeding on the shape,size structure,taxonomic composition and physiological status of picoplankton communities.国家973计划项目课题(No.2011CB409804); 国家自然科学基金项目(No.40876078

Morphological and Molecular Identification of a Marine Flagellate

采用微液滴操作法在厦门海域分离培养了一株海洋鞭毛虫C6,运用显微镜观察和18SrdnA基因技术鉴定其种类,并将其与gEnbAnk中已知的14种海洋鞭毛虫18SrdnA序列比较,用nJ法和uPgMA法构建系统发育树.通过PCr扩增获得1 774bP 18SrdnA序列,与nCbI已登陆的其他同源序列进行系统分析,结果显示此海洋鞭毛虫属于领鞭毛虫(CHOAnOflAgEllATES).与已知种MOnOSIgA brEVICOllIS的相似性为99%,为进一步研究该种鞭毛虫提供了遗传背景,但要明确其种类还需其他研究佐证.Using microscope and 18 SrDNA techniques,a strain of nanoflagellate(C6)separated in Xiamen Bay was identified and analyzed.One 1 774 bp fragment of 18 SrDNA was amplified from the strain C6.Homology analysis between the yielded sequence and the 18 SrDNA sequences accessed in the National Center for Biotechnology Information(NCBI)from other strains showed that C6 belonged to choanoflagellates,which shared 99% homologue with the known species of Monosiga brevicollis.Choanoflagellates play a very important role in biological evolution and marine ecology,however,molecular phylogenetic studies within choanoflagellates are still extremely limited.According to results of morphological and DNA sequence analysis,we consider that C6 should be Monosiga brevicollis,but more collaborations are still required for specific identification.This study provides basic genetic information for further study of physiological characteristics and ecological functions of the strain C6 in Xiamen Bay.国家自然科学基金(41376131); 国家重点基础研究发展计划(973计划)(2011CB409804

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements