双胎妊娠早期糖尿病相关危险因素分析及预测

【目的】探讨双胎妊娠期糖尿病（GDM）早孕期的相关危险因素，以寻找早孕期预测双胎GDM 的有效指标。【方法】回顾性分析2015  年1  月至2019  年6  月在中山大学附属第三医院规律产检并分娩，且病例资料完整的双胎妊娠孕妇共 318 例，根据 75 克口服葡萄糖耐量试验（OGTT）结果，将其分为双胎 GDM 组 84 例，正常双胎组（双胎未合并GDM）234 例，收集记录研究对象的临床特征，包括年龄、孕前体质量指数、孕产次、既往病史、此次受孕方式、双胎绒毛膜性质；早孕期（11~13+6 周）的空腹血糖（FPG）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）及早孕期唐氏筛查生物学标记物妊娠相关血浆蛋白A（PAPP-A）和人绒毛膜促性腺激素β亚单位（Free β-HCG）的检测结果，通过多因素 Logistic 逐步回归分析法分析双胎 GDM 的早孕期相关危险因素，采用受试者工作特征曲线评价双胎 GDM 的早期预测。【结果】①两组比较，双胎 GDM 组的年龄、孕前体质量指数高于正常双胎组（P 0.05）；③ 年龄、孕前体质量指数、PCOS 病史、早孕FPG 水平均为双胎GDM 发生的独立危险因素（P < 0.05）；④早孕期FPG 预测双胎GDM 的ROC 曲线下面积（AUC）为0.617（95% CI：0.547 ~ 0.688），FPG 为 4.495 mmo/L 时，约登指数为 0.223，敏感度、特异度分别为 65.5%、56.8%；早孕期 TG 预测双胎 GDM 的 AUC 为 0.65（95%CI：0.584 ~ 0.716），TG 为 1.195 mmo/L 时，约登指数为 0.24，敏感度、特异度分别为 77.4%、46.6%；而早孕期 FPG、TG 结合孕妇年龄、孕前体质量指数、PCOS 病史预测双胎 GDM 的 AUC 为 0.751 （95% CI：0.693 ~ 0.810），敏感度、特异度分别为73.8%、70.1%。【结论】孕妇年龄、孕前体质量指数、PCOS 病史、早孕FPG 水平均为双胎GDM 发生的独立危险因素；早孕期FPG、TG 可作为早期预测双胎GDM 发生的指标，结合临床特征可以增加早期预测价值

高致病性禽流感病毒血凝素蛋白广谱中和表位模拟肽的筛选与鉴定

以H5N1型禽流感病毒HA蛋白广谱中和单抗8H5为基础,利用噬菌体展示肽库技术及类病毒颗粒融合表达技术研究HA模拟表位。ELISA检测结果显示:筛选获得模拟HA表位的模拟肽123,进行类病毒颗粒融合蛋白表达后,仍具有与8H5单抗特异结合的能力。免疫荧光检测结果说明,类病毒颗粒免疫小鼠后产生了能与HA交叉反应的抗体。禽流感病毒HA模拟表位的研究与性质的分析及类病毒颗粒融合蛋白的表达与活性分析、免疫原性分析,都为研制禽流感通用表位疫苗奠定了基础

Research progress of high voltage electrolyte for lithium-ion batteries

随着对锂离子电池能量密度要求的提高,新一代高电压锂离子电池和与之匹配的高电压电解液的研究开发也日益受到重视。从优化电解液的组成和使用具有特殊功能的新型溶剂、添加剂等方面对锂离子电池高电压电解液的研究进行分析和总结,并对未来的应用前景进行展望。With the increasing requirements for the lithium-ion battery energy density, more and more attentions are focused on the research and development of a new generation of high voltage lithium-ion battery and high voltage electrolyte.The recent research progress related to high voltage electrolyte solutions was analyzed and summarized from the electrolyte composition, special solvent and additives

Comparison of Recoveries of PAHs Obtained by Ultrasound-assisted Extraction from Wet and Freeze-dried Marine Sediment

以美国环保局(EPA)优先控制的16种多环芳烃(polycyclic aromatic hydrocarbons,PAHs)为研究对象,建立了湿法超声波辅助萃取技术提取沉积物中痕量PAHs的可靠方法,对萃取剂、萃取功率、萃取次数和萃取时间进行了优化。并与传统干法超声辅助萃取进行了比较,湿法超声波辅助萃取的PAHs回收率为57%～125%,而干法超声波辅助萃取PAHs的回收率为48%～113%,对相对分子质量小的PAHs如萘～芴的回收率,湿法明显高于干法。该文建立的湿法超声辅助萃取适用于沉积物和泥土中痕量PAHs的快速测定。An ultrasound-assisted extraction method for the extraction of 16 trace PAHs(USEPA priority pollutant) from wet(i.e.untreated) marine sediment was developed.The effects of solvent type,power of ultrasound,and extraction time were studied and optimized.The developed method was compared with the traditional ultrasound-assisted extraction for freeze-dried sediment.The results showed that recoveries of sixteen PAHs extracted from the wet marine sediment ranged from 57% to 125%,whereas those extracted from the freeze-dried marine sediment ranged from 48% to 113%.The results also indicated that the recoveries of PAHs with smaller relative molecular mass(e.g.naphthalene and fluorine) extracted from the wet sediment were higher than those from the freeze-dried sediment.Thus the developed method was appropriate for rapid determination of trace PAHs in marine sediment and soil.海洋公益性行业科研专项(200705011);; 中国海监技术支撑体系项目;; 2008年海洋环境保护及节能减排专

Concentration and distribution of polycyclic aromatic hydrocarbons in surface sediments collected in the Southern Yellow Sea

采用气相色谱与质谱联用(gC/MS)技术,在一个航次内对南黄海表层沉积物中16种优先监控的PAHS的污染状况进行了调查,采用菲/蒽、荧蒽/芘、荧蒽/(荧蒽+芘)、吲哚芘/(吲哚芘+苯并(g,H,I)苝)等特定比值对PAHS来源进行了分析.结果表明,南黄海表层沉积物中检出PAHS的总含量为90.4~732.65ng·g-1,各站点均以4~6环为主;与其它站位相比,倾废区的H01站位受到PAHS污染较为明显,无论是16种PAHS总量还是高分子量组分最高值都出现在该站点,虽然该海区沉积物中PAHS的含量没有超出生物影响低值,但苯并(b)荧蒽、吲哚芘和苯并(g,H,I)芘等一些没有最低安全标准的PAHS也有不同程度的检出,对海洋生物具有潜在的毒副作用.PAHS可能来源于原油、生物和煤燃烧造成的污染.The concentrations of sixteen representative Polycyclic Aromatic Hydrocarbons(PAHs) in surface sediment samples collected from the Southern Yellow Sea were determined by GC-MS.The distribution and potential sources of PAH contamination in the region were investigated.The types of PAHs found in the surface sediments were mainly 4～6 ring PAHs,and they are unevenly distributed among the different sampling sites covering an area of 139 km2.The area is known for continuous waste dumping activities in recent years,and indeed sites adjacent to such activities were found to have high levels of PAHs.Some high molecular weight PAH compounds such as benzo$$b$$fluoranthene,dibenzo$$a,h$$anthracene and benzo$$g,h,i$$perylene were detected in some samples.The health implication of these contaminants needs careful assessment since toxicity guidelines are not available for these species.The potential sources of PAHs are discussed using various source-specific PAH indexes such as Phenathrene/Anthracene、Fluoranthene/Pyrene、Fluoranthene/(Fluoranthene+Pyrene) and Indeno [1,2,3-cd] pyrene/(Indeno[1,2,3-cd] pyrene+Benzo [g,h,i] perylene).The composition of the observed PAHs reflects that they originate from both petroleum utilization and incomplete combustion.海洋公益性行业科研专项(No.200705011);中国海监技术支撑体系项目;2008年海洋环境保护及节能减排专项---

Development of Electrochemical Biosensor for Detection of PML/RARα Fusion Gene in Acute Promyelocytic Leukemia

针对急性早幼粒细胞白血病(APl)中PMl/rArα融合基因的碱基序列,设计了锁核酸(lnA)修饰的发夹结构捕获探针,结合信号探针构建新型的“三明治“电化学传感模式。信号探针末端修饰的生物素可与酶上的亲和素结合,通过检测酶催化H2O2氧化底物3,3',5,5'-四甲基联苯胺(TMb)产生的电化学信号,实现对靶序列的检测。该传感器可识别和定量检测PbS缓冲液中人工合成的PMl/rArα融合基因序列。结果表明,该传感器能很好地区分互补序列、单碱基及多碱基错配序列,杂交电流值与目标链浓度在1.0x10-11~1.6x10-10 MOl/l范围内呈较好的线性关系,检出限为1.0x10-13 MOl/l。同时,该新型传感器成功地用于无稀释人血清中PMl/rArα融合基因的检测,具有特异性强、灵敏度高和重复性好的优点,有望用于临床实际样品的检测,进而实现临床上急性早幼粒细胞白血病的早期诊断及预后判断。A novel DNA electrochemical probe(locked nucleic acid,LNA) was designed and involved in constructing an electrochemical DNA biosensor for the detection of PML/RARα fusion gene in acute promyelocytic leukemia(APL).This biosensor was based on a "sandwich" detection strategy,which involved a pair of LNA probes,e.g.hairpin capture probe and reporter probe.Streptavidin-HRP was bound to biotin labeled at the end of reporter probe via streptavidin-biotin affinity binding.In the presence of hydrogen peroxide(H2O2),HRP catalyzed the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidene(TMB) to offer an enzymatically amplified electrochemical current signal for the detection of target DNA.This sensor was applied in the direct quantitative detection of synthetic PML/RARα fusion gene in PBS buffer.The results indicated that the biosensor showed an excellent specificity to distinguish the complementary sequence and different mismatch sequences.A linear relationship between the amperometric signal and the target concentration was obtained in the range of 1.0×10-11-1.6×10-10 mol/L with a detection limit of 1.0×10-13 mol/L.In addition,the biosensor was used for the determination of PML/RARα fusion gene in human serum samples without dilution with high sensitivity,selectivity and good repeatability.This method would be expected to use in real sample for further solving the actural problems of early diagnosis and prognosis monitoring of APL.863计划资助项目(2008AA02Z433);福建省高校产学研科技重点项目(2010Y4003);国家自然科学基金资助项目(20805006;20975021);福建省自然科学基金资助项目(2010J05019

产时超声作为新的产程评估手段的体验感及接受度调查

目的探讨孕妇对产时超声（IPUS）这一新的产程评估手段的体验感及接受程度。方法对2023年2月至2024年12月入住中山大学附属第三医院岭南医院产房拟行阴道试产的180例孕妇，在临产后、实施分娩镇痛前给予IPUS及阴道检查（VE）进行产程评估，其后通过自制的问卷调查表，调查孕妇对两种检查方法的体验感及对IPUS的接受度，并采用视觉模拟疼痛量表（VAS）对检查的疼痛程度进行评分，比较孕妇对两种产程评估方法的体验感及疼痛程度的差异。结果IPUS的接受度高达96.67%（174/180，其余6例孕妇选择不确定）；60%以上的孕妇认为IPUS评估舒适，且所有孕妇均无不适感，而使用VE评估则有32.8%的孕妇感到不适（χ2=196.02，P＜0.001）。近2/3的孕妇认为VE后会带来心理阴影，而IPUS后无1例孕妇认为会带来心理阴影（χ2=261.52，P＜0.001）。约77.78%（140/180）的孕妇认为若用IPUS代替VE，可以减少其对阴道分娩的恐惧、增强分娩的信心。孕妇对使用的VAS 0（0，2）显著低于使用VE的VAS 4（4，6），差异具统计学意义（Z=-14.62，P＜0.001），进一步进行分层分析显示，90%（164/180）以上孕妇认为IPUS检查无疼痛，无孕妇有中度或以上疼痛感，而进行VE的孕妇中，有43.33%（78/180）有中度或以上疼痛 （P＜0.001）。结论IPUS作为一种新行产程评估方式，能够减少传统VE带来的疼痛不适，降低孕妇对分娩的恐惧、提升分娩信心，且在我国孕产妇中也有很高的接受度

Relationship between genetic lineages and pathotypes of Magnaporthe grisea isolates from Fujian

采用rEP-PCr技术对福建省189个稻瘟病菌菌株进行dnA指纹图谱分析.结果表明:在58%相似水平上可以将供试菌株分为15个遗传谱系,表明该菌具有丰富的多态性.其中谱系fJl04出现频率为35.98%,为优势谱系.利用全国统一生理小种鉴别体系和lTH-nIlS鉴别体系从供试的189个稻瘟病菌株中分别鉴别出15和21个致病型(生理小种),优势型分别为zb13和J76.2,出现频率分别为58.72%和28.57%.采用这2套鉴别体系鉴别的致病型与dnA指纹图谱之间无明显的对应关系.The DNA fingerprints of 189 isolates of Magnaporthe grisea from Fujian were constructed using rep-PCR technique.All isolates could be divided into 15 genetic lineages at 58% similarity level.Among the 15 genetic lineages,FJL04 that consisted of 68 isolates was recognized as dominant lineages with occurrence frequency of 35.98%.The 189 isolates could be respectively classified into 15 and 21 pathotypes by Chinese race differentials and LTH-NILs,and their dominance types were ZB13 and J76.2 with occurrence frequency of 58.72% and 28.57%,respectively.There was no obviously corresponding relationship between pathotypes and genetic lineages.福建省科技厅资助项目(2005N030、2006N0034);福建省农科院青年科技人才创新基金资助项目(200708

高致病性禽流感病毒广谱嵌合抗体的构建表达及活性研究

高致病性禽流感病毒高度变异且缺乏有效的治疗药物.在前期研究工作中,本课题组发现一株鼠源广谱中和单抗13D4,在动物实验中显示出对H5N1禽流感病毒具有广谱治疗效果.在本研究中,从分泌13D4单克隆抗体的杂交瘤细胞株中抽提总RNA,经RT-PCR扩增出轻重链可变区DNA序列,并分别与人IgG1的轻重链恒定区基因序列拼接,构建人-鼠嵌合抗体.筛选出稳定表达嵌合抗体的CHO细胞株,从培养上清中纯化出嵌合抗体.竞争Elisa结果表明,嵌合抗体与鼠源单抗能够识别同一个抗原表位并具有相似的亲和力.血凝抑制反应和中和活性测定结果证明,13D4嵌合抗体保留了对不同亚型H5N1病毒的广谱反应性,并且对两株H5N1病毒具有中和活性.本研究获得的13D4嵌合抗体将具有潜在的治疗价值

养正消积胶囊辅助GP方案治疗晚期非小细胞肺癌临床研究

目的观察养正消积胶囊辅助GP方案治疗晚期非小细胞肺癌(NSCLC)的临床疗效。方法采用随机数字表法将89例晚期NSCLC患者分为对照组44例及观察组45例。2组均采用GP方案;观察组并予养正消积胶囊,每次4粒,每日3次,口服。2组均21d为1个周期,连续治疗2个周期,随访1年。观察2组治疗前后Ki67、Bax、Bcl-2蛋白表达及肿瘤标志物[血管内皮生长因子(VEGF)、骨桥蛋白(OPN)、癌胚抗原(CEA)、糖类抗原199(CA199)]、中医症状评分、生活质量评分,比较2组客观缓解率(ORR)、疾病控制率(DCR)、无进展生存时间(PFS),监测不良反应。结果观察组ORR、DCR明显优于对照组(P<0.05)。与本组治疗前比较,观察组治疗后、随访1年VEGF、OPN、CEA、CA199水平明显降低(P<0.05);对照组治疗后上述指标明显降低(P<0.05),随访1年CEA水平明显降低(P<0.05)。与本组治疗前比较,2组治疗后Ki67、Bcl-2蛋白表达明显降低,Bax蛋白表达明显升高(P<0.05);2组治疗后比较,观察组Ki67、Bcl-2蛋白表达低于对照组(P<0.05),Bax蛋白表达高于对照组(P<0.05)。与本组治疗前比较,2组治疗后中医症状评分(神疲乏力、胃纳少馨、腰膝无力、夜尿频多、头晕目眩)、生活质量评分(躯体功能、认知功能、社会功能、情绪功能)明显改善(P<0.05);2组治疗后比较,观察组上述评分改善明显优于对照组(P<0.05)。观察组PFS明显长于对照组(P<0.05),不良反应明显低于对照组(P<0.05)。结论养正消积胶囊辅助GP方案治疗晚期NSCLC可有效降低患者肿瘤标志物水平,改善生存质量,减少吉西他滨和顺铂的不良反应,提高疗效。福建省卫生厅中医药科研专项课题(wst201210