牵拉力对共培养的表皮干细胞及成纤维细胞迁移行为的影响

背景:创面愈合是一个复杂的动态过程,是细胞之间、细胞与细胞外基质及细胞因子间相互影响和协同作用的结果。另外,大量相关研究表明机械应力可以诱导组织细胞增殖,促进创面愈合。因此创面愈合也是一个典型的力学-生物学耦合过程,但力学刺激促进创面愈合的细胞/分子的调控机制目前尚不清楚。皮肤由多种细胞组成,通过牵张皮肤等在体方法难以区分其中某类细胞对外界力学刺激的单独响应以及

社区管理高血压患者心理健康状况及其影响因素分析

目的了解社区管理高血压患者的心理健康状况及其影响因素,为制定心理干预策略提供依据。方法于2015年12月至2016年3月采用多阶段分层整群抽样方法抽取宁波市海曙区、北仑区和江北区所辖范围内当地基本公共卫生服务系统中的18岁及以上高血压患者1 284例为调查对象。采用患者健康问卷抑郁量表(PHQ-9)和焦虑筛查问卷(GAD-7)评估高血压患者的心理健康状况。采用SPSS 19.0统计软件进行高血压患者心理健康状况影响因素的单因素和多因素logistic回归分析。结果本次调查的1 284名高血压患者中,抑郁阳性率为9.58%,其中轻度抑郁的阳性率为7.87%,中重度抑郁阳性率为1.71%;焦虑阳性率为4.44%,其中轻度焦虑阳性率为3.82%,中重度焦虑阳性率为0.62%。多因素logistic回归分析结果显示,离异或丧偶(OR=2.73,95%CI:1.45~5.14)、合并脑卒中(OR=19.71,95%CI:1.89~205.61)和病程≥16年(OR=1.76,95%CI:1.02~3.05)是高血压患者抑郁的危险因素,而年龄45~59岁(OR=0.32,95%CI:0.16~0.66)、大专及以上文化程度(OR=0.33,95%CI:0.15~0.75)是高血压患者抑郁的保护因素;离异或丧偶(OR=3.73,95%CI:1.71~8.12)、合并脑卒中(OR=15.85,95%CI:1.97~127.30)和合并肿瘤(OR=19.11,95%CI:4.85~75.29)是高血压患者焦虑的危险因素,均有统计学意义(P<0.05,P<0.01)。结论应将慢性病患者心理问题筛查及干预逐步纳入社区卫生服务中,针对不同年龄、文化程度、婚姻状况、病程及合并症的高血压患者采取不同的心理干预策略。浙江省公益性技术应用研究计划项目(2015C33099

Technical advances in space cell bioreactor

为了研究空间微重力环境对生命系统的影响,在空间进行细胞生物学实验是不可或缺的环节。空间细胞生物反应器作为支持空间生命科学研究的重要技术手段,直接影响到空间实验数据的质量和研究水平。迄今为止,空间细胞生物反应器技术仍在发展之中.,尚无统一标准。综述空间细胞生物反应器发展的现状,介绍几类典型空间细胞生物反应器的工作原理,着重分析培养液供应方式和空间流体力学环境的特点,并简要分析对温度、溶氧、溶液PH值等过程参数控制及在线显微观测技术,以期探讨空间生物反应器的未来发展方向。</p

Selectivity of biopolymer membranes using HepG2 cells

Bioartificial liver (BAL) system has emerged as an alternative treatment to bridge acute liver failure to either liver transplantation or liver regeneration. One of the main reasons that the efficacy of the current BAL systems was not convincing in clinical trials is attributed to the lack of friendly interface between the membrane and the hepatocytes in liver bioreactor, the core unit of BAL system. Here, we systematically compared the biological responses of hepatosarcoma HepG2 cells seeded on eight, commercially available biocompatible membranes made of acetyl cellulose&ndash;nitrocellulose mixed cellulose (CA&ndash;NC), acetyl cellulose (CA), nylon (JN), polypropylene (PP), nitrocellulose (NC), polyvinylidene fluoride (PVDF), polycarbonate (PC) and polytetrafluoroethylene (PTFE). Physicochemical analysis and mechanical tests indicated that CA, JN and PP membranes yield high adhesivity and reasonable compressive and/or tensile features with friendly surface topography for cell seeding. Cells prefer to adhere on CA, JN, PP or PTFE membranes with high proliferation rate in spheriod-like shape. Actin, albumin and cytokeratin 18 expressions are favorable for cells on CA or PP membrane, whereas protein filtration is consistent among all the eight membranes. These results further the understandings of cell growth, morphology and spreading, as well as protein filtration on distinct membranes in designing a liver bioreactor.</p

Evaluation on difference of therapeutic efficacy of Jiawei Xiaoyao Granules and Pills in treatment of emotional disorder during perimenopause based on Greene Climacteric Scale

目的:在前期加味逍遥丸治疗围绝经期情绪障碍具有比较优势的基础上,基于grEEnE量表探讨其颗粒剂和丸剂不同剂型间疗效差异。方法:经筛选后患有情绪障碍的围绝经期女性75例随机等分为A、b、C3组,分别对应采用帕罗西汀、加味逍遥丸和加味逍遥颗粒剂治疗8周。所有患者在治疗前后各进行1次grEEnE及其子因子评定,并在观察期结束时进行TESS评定。结果:grEEnE总分差及其焦虑、抑郁因子分差:3种治疗方法均能改善,且相互间无显著差异;grEEnE性因子和血管因子分差:后二组均优于A组(P<0.01);TESS评分:与A组比较,b、C组均有统计学意义(P<0.01),评分均小于A组,且后二组间无显著差异。结论:加味逍遥的2种剂型间疗效无显著差异,考虑到帕罗西汀的不良反应,治疗该病加味逍遥的2种剂型都值得推荐。Objective: To investigate the difference in therapeutic efficacy between Jiawei Xiaoyao Granules and pills based on the preliminary research of treating emotional disorder during perimenopause with Jiawei Xiaoyao Pills and Greene Climacteric Scale.Methods: 75 female patients with emotional disorder during perimenopause were slected and randomly divided into 3 groups as group A, B and C.Patients in the 3 groups were treated with paroxetine, Jiawei Xiaoyao Pills and Jiawei Xiaoyao Granules respectively for 8 weeks.Greene and Greene factors assessment were carried out in all the patients before and after treatment, and the TESS assessment was carried out at the end of the observation period.Results: The three therapies all could improve the gap in total score of Greene and its factors as anxiety and depressed, and the difference among these three groups was no significant.The factors of Greene and gap in score of vascular factors of group B and C was better than that of group A(P<0.01).The difference in TESS score between group A and group B and C was significant(P<0.01), while the difference between group B and group C was not significant.Conclusion: The difference in curative effect between the two formulations was not significant.Because of the adverse reactions of paroxetine, in the treatment of emotional disorder during perimenopause, the two formulations of Jiawei Xiaoyao Powder were worth to be recommended.国家自然科学青年基金项目(No.81302960)~

Fluid flow-induced calcium response in early or late differentiated osteoclasts

Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling

Asymmetric Migration of Human Keratinocytes under Mechanical Stretch and Cocultured Fibroblasts in a Wound Repair Model

Keratinocyte migration during re-epithelization is crucial in wound healing under biochemical and biomechanical microenvironment. However, little is known about the underlying mechanisms whereby mechanical tension and cocultured fibroblasts or keratinocytes modulate the migration of keratinocytes or fibroblasts. Here we applied a tensile device together with a modified transwell assay to determine the lateral and transmembrane migration dynamics of human HaCaT keratinocytes or HF fibroblasts. A novel pattern of asymmetric migration was observed for keratinocytes when they were cocultured with non-contact fibroblasts, i.e., the accumulative distance of HaCaT cells was significantly higher when moving away from HF cells or migrating from down to up cross the membrane than that when moving close to HF cells or when migrating from up to down, whereas HF migration was symmetric. This asymmetric migration was mainly regulated by EGF derived from fibroblasts, but not transforming growth factor alpha or beta_1 production. Mechanical stretch subjected to fibroblasts fostered keratinocyte asymmetric migration by increasing EGF secretion, while no role of mechanical stretch was found for EGF secretion by keratinocytes. These results provided a new insight into understanding the regulating mechanisms of two or three-dimensional migration of keratinocytes or fibroblasts along or across dermis and epidermis under biomechanical microenvironment

一种微型可程控的旋压式夹管阀-,

本发明公开了一种微型可程控的旋压式夹管阀，包括电机固定盘和驱动电机，所述电机固定盘与驱动电机的外壳固定连接，所述电机固定盘上表面由下至上依次设置有若干个泵体，所述泵体边缘上表面设有若干个容纳胶管的卡槽，所述胶管顺次穿过相邻的卡槽并且通过压盖固定在泵体上，所述压盖通过旋进螺栓固定在泵体上，所述泵体中心均设有凸轮盘，所述凸轮盘通过安装孔固定安装有驱动轴，所述驱动轴与驱动电机连接，所述驱动电机还连接有PLC控制系统，所述PLC控制系统与外接计算机电性连接，并接收外接计算机的控制信号来控制驱动电机的转动；可以实现多路通道的同时控制，提高阀门控制的效率，而且能够实现电动的统一控制，更加节能、准确