台美“贸易暨投资框架协议”谈判的历程、特征和走势

2013年3月,停摆达5年之久的台美“贸易暨投资框架协议“(TIfA)重启商谈。此次会谈虽未取得重大成果,但TIfA作为台美之间唯一的高层经贸协商管道,对台美经贸关系的促进,乃至于双方互信关系的提升具有重要作用。本文拟回顾台美TIfA商谈的历程,总结其基本特点,并对其走势作出初步的判断

基于GPP-SDR的HSUPA接收机软件设计及SSE指令优化

文章首先介绍了Td-HSuPA信道,然后对Td-HSuPA接收机的软件设计及SSE优化进行重点分析,并给出了优化前后的运行时间对比。通过对关键算法模块采用SSE指令进行优化,可极大地减少运行时间,提高软件运行效率。IBM开放合作研究项目IBMSUR/OCR(20122X03003007-004

Establishment of a nude mouse model of glioma orthotopic xenograft with double-fluorescent labeling

目的建立一种稳定、可实时监测的胶质瘤原位移植瘤裸鼠模型。方法用带有荧光素酶（luciferase-Luc）和绿色荧光蛋白（green fluorescent protein-GFP）基因的慢病毒感染U251神经胶质瘤细胞,流式细胞仪筛选稳定表达GFP-Luc荧光的细胞系,并通过CCK-8实验、细胞周期实验、Transwell肿瘤迁移及侵袭实验等评价荧光细胞的增殖、迁移和侵袭能力是否改变;将细胞接种至裸鼠大脑尾状核,建立胶质瘤原位移植瘤模型,利用小鼠活体成像系统监测脑内肿瘤的生长情况,并通过石蜡切片,HE染色评价细胞在裸鼠脑内的病理特征及成瘤能力。结果成功构建稳定表达GFP荧光和luciferase荧光的U251胶质瘤细胞系及动物模型,慢病毒整合并未改变细胞的增殖、迁移及侵袭能力;模型生长周期适中,成瘤率高,瘤体在颅内生长稳定,HE切片符合人胶质瘤特征。结论双荧光标记的胶质瘤细胞相比于传统细胞更有利于胶质瘤动物模型的实验研究;U251-GFP-Luc胶质瘤细胞裸鼠模型,其肿瘤生长和病理特性与人胶质瘤相似,且可实时观察肿瘤生长,可作为胶质瘤实验研究的理想动物模型。Objective To establish a stable and real-time monitorable nude mouse model of orthotopic glioma xenograft. Methods U251 glioma cell line was infected by a leutiviral vector containing green fluorescent protein （GFP） and luciferase （Luc） gene. Ceils stably expressing fluorescence of GFP and Luc were sorted by flow cytometry. CCK-8 test and Transwell tumor invasion and migration assay were used to compare the biological features between the ceils stably expressing GFP-Luc fluorescence and cells without fluorescence. Then the ceils were implanted intracranially in the right caudate nucleus of athymic BaIb/c nude mice to establish the tumor model. The growth of intracerebral tumor was monitored over time by a bioluminescence imaging （BLI） system. Hematoxylin-eosin （HE） staining was used to evaluatethe histopathological features and tumorigenicity of the transplanted glioma cells in the brain of nude mice. Results U251 glioma cell line with stably expressing GFP-Luc fluorescence and the corresponding orthotopic xenograft model were successfully established. There was no statistically significant difference in the proliferation, invasion and migration abilities between the cells with stably expressing GFP-Luc fluorescence and the control cells. This model showed a high tumor formation rate and stable tumor growth, and takes a moderate time to establish this model. Conclusions Compared with the traditional glioma cells, GFP-Luc-transfected human glioma cells are more feasible for the studies of glioma in vivo. The tumor growth and pathological characteristics in this U251-GFP-Luc glioma model are similar to human glioma, and the growth of this tumor can be real-time monitored. It can be used as an ideal animal model for experimental studies of glioma.国家自然科学基金（编号：30970733）

Study of Deep-level Fine Structure by Laplace Defect Spectroscopy

用Laplace缺陷谱仪 (LDS)实验研究了GaAsP中Fe深受主上空穴发射和AlGaAs中SnDX中心上电子发射引起的非指数瞬态 ,发现它们起因于混晶无序效应。与DLTS的单一谱峰比较 ,LDS谱呈现出多峰结构。由深能级上空穴与电子热发射率随温度关系的直线拟合 ,得到多峰结构各峰谱的激活能 ,认为它们反映杂质深中心与其近邻原子的不同结构。研究表明 ,LDS适用于深能级精细结构的研究。A Laplace defect spectrometer (LDS) is applied to study non exponential transients resulting from hole emission from Fe related deep acceptors in GaAsP and electron emission from Sn related DX centers in AlGaAs. The non exponential transients which bear a relationship to the alloy random effect are investigated under different conditions. Their LDS spectra exhibit several well resolved sharp peaks assigned to the fine structures of the Fe related deep acceptors and the two DX centers, respectively. The activation energies of the fine structures are determined by linear fitting of the slopes of temperature dependences of hole and electron emission rates. The results show that the LDS is useful for investigation of deep level fine structures.国家自然科学基金资助项目 (6 9976 0 2 3) ;; 福建省自然科学基金资助项

Scrap iron for treatment of reactive dye wastewater

研究了反应时间、染料浓度、进水pH以及不同的废铸铁屑投加量的条件下,废铸铁屑内电解法处理模拟印染废水的脱色能力。并采用铁屑滤床强化厌氧-好氧膜生物反应器(A/O MBR)处理含活性染料的模拟废水。研究结果表明,铁屑对模拟印染废水的最佳脱色时间为12min,酸性条件下铁屑的脱色率优于碱性条件,随铁屑投加量的增加,系统对印染废水的脱色率提高,铁屑滤床强化A/O MBR处理可以提高组合工艺出水色度和COD的去除率。 【英文摘要】 Scrap iron was effective for decolorizing reactive dye wastewater due to the redox reactions taking place near surface of scrap iron which functioned as electrode of the micro-cell systems. The effects of reaction time, dye concentration, influent pH and scrap iron dosage were investigated. The optimum reaction time was 12 min; the decolorization was better when the influent pH was acidic and/or a higher iron dose was employed. Scrap iron addition enhanced performance of the anaerobic-aerobic membrane biore..

CLSI EP15-A3在临床生化正确度验证中的应用

目的探讨美国临床和实验室标准化协会（CLSI）EP15-A3文件在正确度验证中的应用价值。方法按照EP15-A3文件5×5实验设计方案,用IFCC提供的Rela A（水平1）和Rela B（水平2）样品、美国国家标准与技术研究院（NIST）909C参考物质分别验证肌酐（Cr）、尿素（Urea）、尿酸（UA）、总胆固醇（TC）、三酰甘油（TG）、葡萄糖（Glu）的正确度。每个样品每天1批,每批重复5次,共5 d,每个样品获得25个数据。用Grubbs’法计算离群值,单因素方差分析（ANOVA）计算用户的批内不精密度（SR）和实验室内不精密度(SWL),基于SR和SWL计算结果的均值及其标准差,计算靶值（TV）及其标准误差,最后计算出TV的确认区间（VI）,观察各指标检测均值是否在VI内,如通过则用户证明了候选方法的偏差可接受;若不通过则计算均值和TV的相对偏差,观察该相对偏差是否小于用户定义的可接受范围（<1/2 TEa）,若是则证明候选方法的偏差可接受。否则,需查找原因或与厂家联系。结果6个生化项目的测定结果均通过Grubbs’法离群值检查,各项目无离群值。该次验证实验中,除NIST 909C参考物质样品Urea、TC,Rela样品Cr水平2均值不在VI内,但相对偏差均小于1/2 TEa外,其他均值都在VI内。结论用EP15-A3文件进行验证的6个生化项目的正确度均符合临床要求。国家自然科学基金(81572088);;“十二五”国家科技支撑计划(2013BAI02B04,2012BAI37B01);;广东省中医院拔尖人才项目(2014-47);广东省中医院综合标准化示范项目(YN2015BZ10

EZH1/2抑制剂UNC1999对外周血免疫细胞表型的影响

目的探讨EZH1/2抑制剂UNC1999对外周血免疫细胞表型的影响。方法用CCK-8法检测UNC1999作用于外周血单个核细胞（PBMC）后的细胞存活率；多色流式细胞术分析免疫细胞表型。结果相比对照组，UNC1999组经典型单核细胞（CD14++CD16-）比例上调［（19.53±1.79）% vs. （66.60±5.02）%，t=13.31，P=0.006］，中间型单核细胞（CD14++CD16+）和非经典型单核细胞（CD14+CD16+）比例下调［（35.08±3.97）% vs. （15.42±2.89）%，t=6.130，P=0.026；（35.50±3.53）% vs. （8.40±3.12）%，t=25.740，P=0.002］； CD56dimCD16+、CD56domCD16+ NK细胞亚群比例下调［（3.39±0.86）% vs. （0.27±0.06）% ，t=4.882，P=0.040；（80.50±0.64）% vs. （0.63±0.23）% ，t=133.100，P&lt;0.000 1］；初始B细胞比例上调［（10.67±1.76）% vs. （37.99±3.76）%，t=17.690，P=0.003］，记忆B细胞、过渡B细胞、浆细胞比例下调［（23.39±4.20）% vs. （11.82±1.90）%，t=7.059，P=0.020；（3.58±0.47）% vs. （1.52±0.56）%，t=26.970，P=0.001；（0.18±0.03）% vs. （0.00±0.00）%，t=8.647，P=0.013］；DC比例上调［（0.20±0.05）% vs. （ 1.38±0.13）%，t=16.500，P=0.004］，其中pDC/DC下调［（24.90±1.95）% vs. （12.70±2.11）%，t=7.566，P=0.017］，mDC/DC上调［（32.41±13.14）% vs. （60.87±8.43）%，t=8.252，P=0.014］；CD8+T细胞亚群中CD8+中枢记忆T细胞、CD8+PD-1+比例上调［（5.62±1.24）% vs. （18.38±2.34）%，t=15.600，P=0.004 ；（2.50±1.02）% vs. （18.34±2.69）% ，t=8.822，P=0.013］，CD8+效应记忆T细胞比例下调［（28.27±10.15）% vs. （15.62±9.48）% ，t=19.480，P=0.003］；CD4+T细胞亚群中CD4+CD27+比例下调［（82.77±2.66）% vs. （56.00±9.01）%，t=5.715，P=0.029］。其余细胞亚群差异无统计学意义（P&gt;0.05）。结论UNC1999可以改变PBMC免疫细胞表型

骨髓间充质细胞联合PDMS支架构建移植胰岛微环境的实验研究

目的为了提高移植胰岛的活性和功能,构建适合移植胰岛生存的微环境。方法采用聚二甲基硅氧烷(PDMS)和氯化钠晶体构建三维支架,联合骨髓间充质细胞(MSCs)、纤维蛋白和胰岛共同构建迷你\"人工胰腺\"。采用链脲佐菌素(STZ)诱导的糖尿病大鼠移植模型评价效果,将\"人工胰腺\"移植到糖尿病大鼠大网膜内,对照组行假手术,术后隔天监测移植大鼠血糖水平;数据采用t检验和曼-惠特尼U检验。结果用PDMS构建的三维巨孔支架,支架内可见大量不规则孔洞空间。胰岛和MSCs可成功装载入支架内,HE染色结果显示,支架孔内存在胰岛,胰岛周围包绕有MSCs。糖尿病大鼠大网膜内移植结果显示,移植后各时间点(1,3,5,7 d),\"人工胰腺\"移植组糖尿病大鼠血糖水平分别为(278.70±86.06) mg/dl、(323.50±44.29) mg/dl、(283.30±74.00) mg/dl、(304.80±13.33) mg/dl,较假手术对照组(606.00±52.40) mg/dl、(589.70±55.78) mg/dl、(615.00±54.84) mg/dl、(630.30±48.17) mg/dl均降低,差异具有统计学意义(t=7.96、9.15、8.82,U=0.00,P均<0.01)。结论 MSCs联合PDMS三维支架构建的微环境,可为移植胰岛提供生存的环境,为临床开展胰岛移植提供新的策略

Research Development of Mechanical Alloying and Amorphous Alloy

[中文文摘]机械合金化是一种通过高能研磨实现的固相粉体加工技术。现已证明可以通过对纯组元混合粉或预合金粉进行机械合金化处理,合成包括非晶合金在内的多种平衡与非平衡合金相。主要评述了机械合金化法在非晶态合金材料研究领域的优势和特点,重点介绍了当前有关机械合金化致非晶化机理的研究成果以及未来这一领域的发展方向。[英文文摘]Mechanical alloying (MA) is a solid-state powder processing technique involving repeated welding and fracturing of particles in a high-energy ball mill. It has been shown to be capable of synthesizing a variety of equilibrium and non-equilibrium alloy phases including the amorphous alloy from blended elemental or prealloyed powders. Mechanical alloying is one of the most potential method in the preparation and science research of amorphous alloy. The characteristics and superiority of mechanical alloying on amorphous alloy research are discussed in this article. Additionally , it int roduces the present research status of the mechanism of amorphization and the development direction.福建省自然科学基金项目(E0310021); 福建省科技计划项目(2002I018)