液相色谱-串联质谱前驱离子扫描非靶向筛查卤代有机物

建立了液相色谱-串联质谱(LC-MS/MS)前驱离子扫描(PIS)非靶向筛查卤代有机物的分析方法。卤代有机物在PIS分析中产生的卤素碎片离子能通过PIS Q3预设的质核比通道(m/z 35、37、79、81和127),根据通过的质核比通道可判断卤代有机物种类,并获取其分子离子质核比信息,而非卤代有机物无法通过PIS Q3的筛选。当Q2碰撞能为50 eV时,PIS对卤代有机物具有较高的灵敏度。利用伯努利概型总结了不同卤代有机物的理论同位素峰数量和丰度比的规律,结合PIS分析结果可判断有机物携带卤素原子数量。本方法成功应用于实验室样品和海水中卤代有机物的筛查,未来有望用于其它环境样品中卤代有机物的快速筛检。国家自然科学基金项目(Nos.21477115,21876142)资助~

Photosynthetic capacity of three common species of macroalgae and the application in coral aquarium

利用水下饱和脉冲调制叶绿素荧光仪(dIVIng-PAM)测定了珊瑚养殖水处理中3种常见大型海藻石莼(ulVA lACTuCA)、条浒苔(EnTErOMOrPHA ClATHrATA)和羽状蕨藻(CAulErPA MEXICAnA)光能转化效率(fV/fM),快速光曲线(rlC)相关参数和非光化学淬灭(nPQ)。结果表明:石莼和条浒苔的fV/fM分别为0.808±0.004和0.816±0.009,显著高于羽状蕨藻。最大相对电子传递速率rETrMAX(17.52±2.92和19.59±4.43μMOl E·M-2·S-1)、半饱和光强Ik(53.41±8.18和59.71±13.52μMOl PHOTOnS·M-2·S-1)也明显高于羽状蕨藻(rETrMAX和Ik分别为13.72±5.41μMOl E·M-2·S-1和32.67±14.06μMOl PHOTOnS·M-2·S-1),表明石莼和条浒苔有着比羽状蕨藻更高的光合能力和对强光的耐受能力,光保护能力更强;羽状蕨藻较高的rlC初始斜率α表明其在弱光下拥有更强的光捕获能力,弱光下光合效率更高;随光照的增强(0~373μMOl PHOTOnS·M-2·S-1),羽状蕨藻nPQ的增幅有限(0~0.2),最大值仅为条浒苔和石莼的38.1%和62.5%,表明这种藻类更容易受到强光的抑制。因此,在珊瑚养殖的水处理中,可以根据不同的光照选择适宜的藻种或藻种组合并根据光梯度进行布局,反之也可以根据藻种来选择合适的光源,以达到最佳的处理效率。This study investigated the difference of photosynthetic capacity in three macroalgae species( Ulva lactuca,Enteromorpha clathrata,and Caulerpa mexicana) commonly used for nutrient removal in coral aquariums.The photochemical efficiency( Fv/Fm),parameters relevant to rapid light curve( RLC) and non-photochemical quenching( NPQ) in the macroalgae were measured by underwater saturation pulse modulated chlorophyll fluorometer( Diving-PAM).The results showed that Fv/Fmwas 0.808 ± 0.004 for U.lactuca and 0.816 ± 0.009 for E.clathrata,which were higher than that for C.mexicana.Higher maximum relative electron transfer rates( rETRmax)( 17.52 ±2.92 and 19.59 ±4.43 μmol e·m- 2·s- 1) and half-starved value of light intensities( Ik)( 53.41 ±8.18 and 59.71 ±13.52 μmol photons·m- 2·s- 1) were observed in U.lactuca and E.clathrata than that in C.mexicana( 13.72 ± 5.41 μmol e·m- 2·s- 1for rETRmaxand 32.67 ± 14.06 μmol photons·m- 2·s- 1for Ik),indicating that U.lactuca and E.clathrata have stronger photosynthetic capacities,photo-protection capacities and tolerance to higher intense light than C.mexicana.A significantly lower RLC initial slope α in C.mexicana implied that this algal species has a higher photosynthetic capacity under weak light condition.With increasing the light intensity( 0- 373 μmol photons·m- 2·s- 1),however,C.mexicana had a limited increase of NPQ( 0- 0.2),the maximum of which was equivalent to 38.1% of that in U.lactuca and 62.5% of that in E.clathrata.These results indicate that the photosynthesis of E.clathrata may be more easily limited by intense light.Our study suggests that the selection of algal species for nutrient removal in a coral aquarium should be adapted to the light intensity.Single species or the combination of different species of macroalgae can be selected according to the illumination of the coral aquarium in order to maximize the efficiency of nutrient removal.国家海洋局第三海洋研究所基本科研业务项目(海三科2011033); 厦门海洋研究开发院共建项目; 海洋公益性行业科研项目(201105012)资

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

溶菌酶1在小鼠超数排卵前后孵化及休眠囊胚中差异表达的研究

目的研究溶菌酶1(lysozyme 1,LYZ1)基因在超数排卵前后小鼠孵化囊胚和休眠胚胎中的分布以及表达,探究动物胚胎着床过程中新的调节机制。方法从妊娠5 d ICR小鼠体内获取的正常孵化囊胚和超排囊胚,利用小鼠延迟着床模型于妊娠第8天获取休眠胚胎和超排休眠胚胎。利用免疫荧光和Western Blot方法检测LYZ1蛋白在四组胚胎中的分布和差异表达变化。结果 LYZ1在超数排卵前、后小鼠孵化囊胚和休眠胚胎中均有表达,且主要集中在内细胞团中,滋养层细胞和胞质中少见分布;与未进行超排的小鼠相比,LYZ1蛋白在超排后小鼠胚胎中表达量显著上调,与未营造休眠模型的小鼠相比,LYZ1蛋白在休眠模型小鼠胚胎中的表达量显著上调。结论 LYZ1蛋白在囊胚内细胞团中表达,可能参与调节胚胎内细胞团的发育;LYZ1蛋白在超排-休眠胚胎中的高表达,说明LYZ1蛋白在休眠和超数排卵的双重影响下,会因为抵御不利环境而上调。2016年度科技创新服务能力建设-科技计划重点项目(KZ201610020018);;2018科技创新服务能力建设(KZ201610020018)~

SARS的激素治疗与胸片所见

【目的】探讨严重急性呼吸综合征（SARS）的糖皮质激素治疗与胸片所见的关系。【方法】收集广州市458例SARS患者的一般临床资料及每次胸片资料。所有患者都临床确诊并经免疫学检查证实,临床资料及胸片资料同时较齐全才作为研究对象。根据应用激素与否将其分为两组。【结果】应用激素组257例,未用激素组201例。两组在首次见到肺内病变时间之差异无统计学意义（t=1.417,P=0.157）;激素用于临床表现较严重的病例,胸片上肺内病变达高峰时间较未用激素组长（t=3.242,P=0.001）,所累及的肺小区个数较多（t=3.982,P〈0.001）,住院时间较长（F=2.731,P〈0.001）。始用激素于病程10d之内及10d之后比较,后者肺内病变达高峰时所累及的肺小区个数较多。最大日剂量较大者,其病变存留较久、住院时间较多（F=3.040,7.044;P〈0.001,〈0.001）。【结论】激素用于症状较重的SARS患者的情况下,病死率不高,提示激素在SARS治疗中有重要意义

Are Conflicts with Supervisor Always Detrimental? Differentiated Effects of Supervisor-subordinate Relational and Task Conflicts on Shared Mental Modelsand Team Performance

上下级冲突的研究大多仅关注冲突如何对当事人产生负面影响。与此不同,本研究探讨团队领导与上级部门领导之间的上下级冲突如何影响团队运作效能。以132个负责通讯网络监控与维护的高技术团队为研究对象,回归分析结果表明,团队队长与上级部门领导之间的上下级任务冲突和共享心智模型以及团队绩效正相关,而关系冲突则负向影响共享心智模型和团队绩效;上下级冲突对团队绩效的影响显著大于共享心智模型对团队绩效的影响。作者从上下级冲突如何影响团队的整体运作,以及任务冲突和关系冲突何时对团队产生不同影响等几个角度展开讨论。</p

悬挂式长短可调鼠尾取血固定装置

本实用新型涉及一种悬挂式长短可调鼠尾取血 固定装置，它包括：一个前头开口的玻璃罩、烧瓶夹、一个圆 筒和后盖，其圆筒上部一侧壁上开有一条形槽，下部侧壁上对 称设置一凸起或开有螺孔；圆形玻璃罩安装在圆筒内，并用烧 瓶夹夹在圆形玻璃罩的颈部，烧瓶夹的螺杆穿出条形槽用螺母 固定在圆筒壁上；其后盖上有一个可使鼠尾穿过的孔，该后盖 套在塑料筒的尾端固定。该固定装置可根据动物的身长调节从 玻璃罩到圆筒尾端的距离，一种型号就可以满足多种种系和日 龄的大鼠采血要求的需要。采血时动物处于头上尾下的悬挂状 态，在重力的作用下， 静脉自然充盈，不需加热，并对动物的制动效果满意，可以满 足大部分动态检测的要求