The study of pancreas three-dimensional reconstruction based on the Virtual Chinese Human-Female No 1

目的研究数字化虚拟胰腺及其周围结构图像的三维重建,创建三维可视化胰腺解剖学,为虚拟胰腺手术及临床胰腺外科提供数字化三维解剖学依据。方法采用南方医科大学虚拟中国人女性一号(VCH-F1)腹部胰腺段影像数据集,以现代看图软件(ACDSee)及图像处理软件(Photoshop)对图像进行分割,编程提取、三维重建胰腺并实现胰腺三维可视化。结果一次完成胰腺及与胰腺关系极为紧密的周围结构:十二指肠、胆总管、下腔静脉、门静脉主干及主要属支、腹主动脉、腹腔干动脉及分支、肠系膜上动脉的三维重建,并实现任意视角下的三维可视化,三维胰腺真实、立体感强,能三维可视化,以全新的胰腺三维结构反映复杂多变的胰腺外形及毗邻结构关系。结论基于VCH-F1的胰腺三维重建及三维可视化,以三维可视化的形式真实再现了胰腺及胰腺周围重要结构,是创建全新的三维可视化胰腺解剖学,虚拟胰腺手术及临床胰腺外科提供数字化三维解剖学依据的理想方法。Objective To study the three-dimensional(3D) reconstruction and 3D visualization of the pancreas and create anatomy of the digitalized visual pancreas so as to construct a concrete basis for virtual operation and surgical operation on pancreas.Methods The digital imaging data of pancreas,duodenum,common bile duct,arteries and veins were obtained from the Virtual Chinese Human-Female 1(VCH-F1) and processed using ACDSee and Photoshop so as to reconstruct 3D pancreas digitally and realize 3D visualization of pancreas.Results We successfully 3D reconstructed and visualized the pancreas and the peri-pancreatic structures: the duodenum,the common bile duct,the inferior vena cava,the portal vein vessels,the aorta,the ceoliac trunk vessels.The 3D and visualized pancreas manifested itself with its complete structure as well as its adjacency to other tissues.Conclusions The 3D reconstruction and 3D visualization of the pancreas based on the digital data of VCH-F1 produces a digitally visualized pancreas,which promises us a novel method for virtual operation on pancreas,clinical operation on pancreas and anatomy of 3D visualized pancreas.国家自然科学基金资助项目(30470493);; 广东省科技计划资助项目(2003C34303

浙江龙王山安吉小鲵的产卵场微生境选择与卵孵化的影响因素

全球两栖动物种群的急剧衰退已成为保护生物学领域的一个核心问题。产卵场选择是两栖动物生活史中至关重要的过程，受到多种环境因素的影响，直接关系到后代的存活率和亲本的适合度。安吉小鲵（Hynobius amjiensis）为国家一级重点保护野生动物，分布范围狭窄、种群数量极少，目前缺乏对该物种产卵场微生境选择的相关研究。2023年11月—2024年3月，本研究团队在浙江安吉小鲵国家级自然保护区内的14个潜在繁殖池监测了安吉小鲵的产卵和孵化过程，并采用主成分分析和广义相加混合模型分析了该物种产卵场选择偏好、卵孵化成功率以及孵化时长等主要影响因素。结果表明：安吉小鲵依据多种环境因子选择筑巢地点，其中，在繁殖池内的相对位置（水体深度、距水面距离、距岸边距离）和繁殖池水质（水体pH、水体溶解性总固体、水体氧含量）是影响安吉小鲵产卵场微生境选择的关键因素；水体温度、水体氧含量和水深等对卵孵化率具有显著影响；卵孵化时长显著受到水体温度和水体pH的影响。研究结果揭示了安吉小鲵对繁殖场的选择偏好，可为安吉小鲵种群的复壮提供数据支持

Digitized virtual liver and hepatic surgery after filling hepatic duct systems

目的:肝脏管道灌注后进行数字化虚拟肝脏及其手术的研究.方法:用四种颜色的灌注材料对肝脏的四种管道分别进行灌注和固定,然后进行包埋、冰冻和铣切,获取连续肝脏断面图像数据集.再将数据集的图像经配准和分割后在VTK的基础上,使用面绘制和体绘制相结合的方法,进行三维重建和虚拟切割实验.结果:获取肝脏连续肝脏断面图像910张,且四种管道颜色不同.三维重建肝脏模型形态逼真,能随意旋转、放大和缩小,通过设定肝脏及其各管道结构的透明度和颜色能单独或组合显示各结构.并且在模型上能行虚拟肝脏切割实验.结论:不同颜色灌注材料对肝脏管道灌注后铣切获取连续断面数据集有益于肝实质及其内部各管道的识别.以此进行三维重建和虚拟手术的研究,有益于肝脏解剖学和肝脏外科的研究.AIM: To study the digitized virtual liver and hepatic surgery after filling hepatic duct systems. METHODS: Four kinds of intrahepatic ducts were perfused with different filling material ex vivo respectively. And then the samples were used to be frozen, embedded and sliced to obtain serial sectional images. The whole series of hepatic images was employed to reconstruct a 3 dimensional (3D) liver. After the image registration of the tissue sections, the image information of liver parenchyma, hepatic veins, bile duct and cholecyst was obtained by image segmentation, followed by the 3D reconstruction using Visualization ToolKit (VTK) to construct a 3D morphological model of the liver surface and intrahepatic structures. The virtual liver could also be resected at any angle to observe the structure of the resection surface. Furthermore, a Windows PC-based 3D visualized demonstration system and Windows operation system of liver were developed. RESULTS: Total 910 consecutive sectional images of liver were taken. Hepatic arteries, inferior cava veins/hepatic veins, portal veins and biliary ducts were shown with red, blue, green, and yellow colors, respectively. This demostration system of liver provided a graphic user interface to rotate and scale the 3D liver to observe 3D morphological features of the liver and its duct systems by setting the pellucidit and color. A virtual liver simulation system for resection with primary function was established. CONCLUSION: The technique of perfusion of hepatic ducts may be beneficial to identification of intrahepatic structure. And research on digitized virtual liver and virtual hepatic surgery may be beneficial to the development of the hepatic anatomy and surgery.国家自然科学基金(30470493);; 广东省科技计划项目(2003C34303