6 research outputs found
X-선 결정학에 의한 황색포도상구균 유래 SAV0324 단백질의 구조 연구
Get PDF학위논문 (석사)-- 서울대학교 대학원 약학대학 약학과, 2017. 8. 이봉진.Staphylococcus aureus is a gram positive bacteria which
produces enzymes, toxins, and small RNAs that are often harmful to
the body. About 20-30% of the human population are carriers of the
bacteria and the bacteria is responsible for causing many diseases
and casualties worldwide.
SAV0324 is a glycine cleavage system H-like protein
(GcvH-L) which acts as a carrier for lipoyl moiety. SAV0324
undergoes lipoylation by SAV0327 protein and subsequently undergoes
ADP-ribosylation process. Without ADP-ribosylation, the virulence of
Staphylococcus aureus bacteria decreases and therefore, inhibition of
lipoylation would be crucial.
Using Structure Based Drug Design (SBDD) as a background
knowledge, the structure of SAV0324 protein was determined at
1.88Å. Specifically, SAV0324 conserved five alpha-helices and nine
beta-strands in its structure and the site of lipoylation in SAV0324
was already determined to be E53 (53rd glutamate) and K56 (56th lysine) residues by other researchers. Furthermore, crystallization of
SAV0327 protein was successful. Through binding test between
SAV0324 and SAV0327, the results showed the two proteins did not
have direct binding interaction in the absence of lipoic acid. Thus, in
addition to SAV0324s structural information, determination of
SAV0327 protein structure and its active site would be of great
significance. Knowing both SAV0324 and SAV0327 thoroughly would
be critical in controlling and understanding bacterial virulence of
Staphylococcus aureus.I. General Introduction ············································ 1
1.1 Staphylococcus aureus ··········································· 1
1.2 SAV0324 ········································································· 2
1.3 Structure Based Drug Design ···························· 3
II. Purpose of Study ················································· 5
III. Materials and Methods ···································· 6
2.1 Materials ········································································ 6
2.1.1 Reagents ···········································································6
2.1.2 Apparatus ·········································································6
2.2 Methods ·········································································· 7
2.2.1 Cloning ··············································································7
2.2.2 Overexpression Test ·····················································8
2.2.3 Solubility Test ································································9
2.2.4 Affinity Chromatography (IMAC) ·····························9
2.2.5 Size Exclusion Chromatography (SEC) ··················· 10
2.2.6 Dialysis and Concentration ··········································11
2.2.7 MALDI-TOF ···································································12
2.2.8 Circular Dichroism (CD) ··············································12
2.2.9 Crystallization ··································································13
2.2.10 Structure Determination ···············································13
IV. Results ·································································· 14
3.1 Polymerase Chain Reaction (PCR) ··················· 14
3.2 Overexpression and Solubility ···························· 15
3.3 Purification (IMAC) ················································ 17
3.4 MALDI-TOF ······························································ 18
3.5 Circular Dichroism (CD) ······································· 19
3.6 Purification (SEC) ···················································· 20
3.7 Crystallization ···························································· 21
3.8 Structure Determination ········································ 22
3.8.1 SAV0324 Structure ··························································22
3.8.2 Crystal Data Collection & Refinement ·······················24
3.8.3 Ramachandran Plot ··························································25
3.8.4 Topology ·············································································26
3.8.5 Homology Search ·····························································27
3.9 Active Site ·································································· 29
3.10 Binding Test ······························································ 30
3.11 SAV0327 ······································································· 31
3.11.1 Cloning, Overexpression, and Solubility ·················· 31
3.11.2 Purification (IMAC & SEC) ········································34
3.11.3 Crystallization ··································································36
V. Discussion ····························································· 38
VI. References ··························································· 41
국문초록 ········································································ 44Maste
String type touch sensor module
No full text본 발명의 터치 센서 모듈은 단일점에 대한 터치를 감지하기 위한 다수의 단일점 터치 센서 및 구동 신호를 상기 각 단일점 터치 센서에 전달하는 다수의 시간 지연 소자를 포함하며, 상기 각 시간 지연 소자와 각 단일점 터치 센서가 병렬로 연결되어 스트링 형태로 배열되며, 상기 구동 신호가 상기 시간 지연소자에 입력되어 스트링 형태의 배열에 따라 전달됨에 따라 순차적으로 상기 단일점 터치 센서들이 활성화된다. 본 발명에 의하면 일차원 멀티 터치 센서라고 할 수 있는 스트링 형태의 터치 센서 모듈을 제공함으로써, 별도의 설계나 제작공정 없이 다양한 형태의 곡면에 터치 센서를 적용하여 디자인할 수 있는 효과가 있다
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No full text본 발명은 곡면 디스플레이 장치에 이용되는 보간재 및 이를 이용한 곡면 디스플레이 장치와 이를 통하여 동적 곡면을 구현하는 방법에 관한 것으로서, 본 발명의 보간재는 굽힘에 대한 저항값이 크고, 늘임에 대한 저항값이 작은 것이고, 보간재는, 복수의 비연성 및 비탄성의 비변형부; 및 상기 복수의 비변형부 사이에 위치하는 변형가능한 변형부;를 포함하는 것일 수 있다
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