Draft Genome Sequence of Vibrio parahaemolyticus SNUVpS-1 Isolated from Korean Seafood

Vibrio parahaemolyticus is the leading cause of food-borne diseases, and several pathogenic strains cause global gastroenteritis outbreaks. Here, we report a draft genome sequence of V. parahaemolyticus SNUVpS-1, which was isolated from seafood in a fishery market in the Republic of Korea and contained TL, toxR, and toxRSold genes. The current draft genome sequence will contribute to the effort to monitor the spread of V. parahaemolyticus seafood isolates and clinical isolates.OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/5SEQ:5PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000030777ADJUST_YN:NEMP_ID:A076079DEPT_CD:551CITE_RATE:0FILENAME:Genome Announc.-2013-Jun-.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:NCONFIRM:

Body extract of tail amputated zebrafish promotes culturing of primary fin cells from glass catfish

The most spectacular regenerative events in vertebrates are epimorphic regeneration. In this study, interestingly, a whole-body extract 24 h after tail amputation enhanced primary cell growth and viability compared to that of a non-tail amputated body. Additionally, these effects of extract treatment in vitro were dose-dependent occurring at concentrations of 0.02, 0.05 and 0.1 mg/ml. This is the first in vitro study on the interaction between primary fin cells from glass catfish and tail amputated body extracts of zebrafish. These results provide an essential knowledge base for rational approaches to tissue and organ regenerationThis study was financially supported by a Korean Research Foundation Grant (KRF-2008-331-E00385).OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/6SEQ:6PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000030777ADJUST_YN:NEMP_ID:A076079DEPT_CD:551FILENAME:Han et al.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates

Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies.OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/2SEQ:2PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000030777ADJUST_YN:NEMP_ID:A076079DEPT_CD:551CITE_RATE:1.5FILENAME:viruses-05-00568 인쇄본.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:NCONFIRM:

Antimicrobial effect of hypochlorous acid on pathogenic microorganisms

Disinfection is essential in terms of the public health or environmental hygiene. Hypochlorous acid (HOCL) solutionwas developed as a disinfectant in Korea. We evaluated the germicidal activity of HOCL against various pathogenic microbes.Thirty-one ATCC strains were exposed to HOCL solution at various concentrations (20, 40 and 80 ppm) for 1 minute. Allthe strains of bacteria, yeasts and mycobacteria were killed at 80 ppm after exposure to HOCL. The results suggest thatHOCL solution could be used to effectively disinfect public areas.OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000030777/7SEQ:7PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000030777ADJUST_YN:YEMP_ID:A076079DEPT_CD:551CITE_RATE:0FILENAME:10 Short Communication 박세창_가편집_1교_저자.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:NCONFIRM:

Complete genome sequence of multidrug-resistant Staphylococcus sciuri strain SNUDS-18 isolated from a farmed duck in South Korea

Objectives: This study aimed to determine the complete genome sequence of multidrug-resistant Staphylococcus sciuri strain SNUDS-18 isolated from a farmed duck in South Korea.Methods: Genomic DNA was sequenced using a PacBio RS II system. The obtained genome was annotated and antimicrobial resistance and virulence genes were identified.Results: The sequenced genome possessed a mecA homologue (mecA1) that was almost identical to that of other oxacillin-susceptible S. sciuri strains, whereas the staphylococcal cassette chromosome mec (SCCmec) was not detected. Moreover, various antimicrobial resistance genes conferring resistance to beta-lactams, aminoglycosides, phenicols, tetracycline and macrolide-lincosamide-streptogramin B (MLSB) antimicrobials were identified.Conclusions: The SNUDS-18 genome and its associated genomic data will provide important insights into the biodiversity of the S. sciuri group as well as valuable information for the control of this potential pathogen. (C) 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.OAIID:RECH_ACHV_DSTSH_NO:T201716233RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:2.022DEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Immunostimulation of Cyprinus carpio using phage lysate of Aeromonas hydrophila

Over the last 50 years, various approaches have been established for the development of antigens for immunostimulation. We used phage lysate (PL), composed of inactivated antigens by the lytic bacteriophage pAh 6-c for Aeromonas hydrophila JUNAH strain to develop a vaccine for the prevention of A. hydrophila infection in Cyprinus carpio (common carp). We also assessed the poly (D,L) lactide-co-glycolic acid (PLGA) microparticles encapsulation method to increase the efficiency of the vaccine. Six groups of vaccines involving encapsulated by PLGA, formalin killed cells, or phage lysate at low or high concentration were prepared for intraperitoneal injection in C. carpio. Blood specimens and head kidney samples were collected at various time points for bacterial agglutination assay and to assess relative expression of immune-related genes interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), lysozyme C, and serum amyloid A (SAA). The vaccine groups using high dose phage lysate antigen showed significantly higher agglutination titers than all other groups at 4- and 6-weeks post vaccination (wpv), with the titer of the PLGA encapsulated vaccine group being highest from 10 wpv to the end of the experiment. The survival rate of fish immunized with the phage lysate vaccines were higher than that of fish immunized with the formailin killed cells vaccine in the challenge experiment conducted 6 wpv. Additionally, the PLGA-encapsulated high dose phage lysate antigen vaccinated groups showed the best protective efficacy in the challenge experiment 12 wpv. Vaccines using the phage lysate antigen also showed higher IL-1 beta and lysozyme C gene expression at 7 days post vaccination (dpv) and 2 wpv, and higher TNF-alpha gene expression was seen at 7 dpv. Higher SAA gene expression was seen in these groups at 1 dpv. These results suggest that phage lysate antigen has the potential to induce robust immune responses than formalin killed cells-based vaccines, and could be more effective as a novel inactivated antigen in preventing A. hydrophila infection in C. carpio.OAIID:RECH_ACHV_DSTSH_NO:T201901278RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:3.185DEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Use of bacterial subcellular components as immunostimulants in fish aquaculture

Aquaculture and fisheries products are expected to play a leading part in addressing the food necessities of the increasing human population. However, in intensive aquaculture, fish are exposed to stress conditions that deteriorate their immune systems, leading to increased disease vulnerability. These disease outbreaks have resulted in production losses and represent one of the key constraints in fish farming. Accordingly, aquaculture disease management efforts have concentrated on the establishment of environmental friendly and lasting methods. Recently, several studies have revealed that various bacteria (especially probiotics)-derived cellular components might be used as promising immunostimulants in fish aquaculture. Such components curtail the risk concomitant with the use of antibiotics, chemotherapeutics, vaccines and probiotics. Among the various health advantages ascribed to bacterial cellular components, the modulation of specific and nonspecific immune responses, cytokine gene expression and anti-pathogenic activities are most anticipated benefits. Their potential as vaccine adjuvants for fish culture has also been demonstrated. However, as an emerging practice, limited knowledge is available regarding the detailed interactions with fish immune systems. Therefore, the underlying mechanisms involved in fish immune cell activation need to be explored further. In this context, the present article aspires to review the available studies on the use of bacterial cellular components in fish culture and their biological effects on fish such as growth promotion, immunostimulation and anti-pathogenic activities as well as to evaluate the factors that control the optimal induction of immune responses in fish. In addition, future issues that merit further investigation are suggested.OAIID:RECH_ACHV_DSTSH_NO:T201606737RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:7.139DEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Role of dietary ginger Zingiber officinale in improving growth performances and immune functions of Labeo rohita fingerlings

This study evaluated the effects of ginger (Zingiber officinale) as a feeding supplement on the growth, skin mucus immune parameters, and cytokine-related gene expression of Labeo rohita, and its susceptibility to Aeromonas hydrophila infection. Diets containing six different concentrations of dried ginger (0% [basal diet], 0.2% [G2], 0.4% [G4], 0.6% [G6], 0.8% [G8], and 1.0% [G10] were fed to fish (average weight: 12.3 g) for 60 days. Growth parameters were examined at 30 and 60 days post-feeding. Skin mucosal immune responses and gene expression were examined 60 days post-feeding. Results showed that growth parameters such as final weight gain (93.47 +/- 1.73 g) and specific growth rate (3.41 +/- 0.14) were significantly higher in G8 than in the control. Among the skin mucosal immune parameters examined, lysozyme (46.5 +/- 3.8 U mg(-1)), immunoglobulin level (8.9 +/- 0.4 unit-mg mL(-1)), protein level (44.3 +/- 2.2 mg mL(-1)) were significantly higher in G8. However, alkaline phosphatase activity (171.6 +/- 10.2 IU L-1) was high (P < 0.05) in the G10 group. Skin mucus of G8 exhibited significantly higher inhibition zones when tested against pathogenic bacterial strains. For cytokine-related genes, antioxidant genes (zinc/copper superoxide dismutase [SOD1], glutathione peroxidase [GPx], anti-inflammatory cytokines (interleukin-10 [IL-10], transforming growth factor-beta [TGF-beta]), signalling molecules nuclear factor erythroid 2-related factor 2 [Nrf2], and Inhibitor protein kappa beta alpha [I kappa B-alpha]) were all up-regulated in the head kidney, intestine, and hepatopancreas of fish that were fed experimental diets. In addition, expression abundance was significantly higher in most tissues in G2 and/or G10, than in the control. Conversely, expression of genes encoding pro-inflammatory cytokines (IL-1 beta, tumour necrosis factor-alpha [TNF-alpha]), signalling molecules Kelch-like-ECH-associated protein 1 (Keap1), and nuclear factor kappa B p65 (NF-kappa Bp65) were down-regulated in treatment groups. Moreover, fish fed a 0.8% [G8] ginger supplemented diet exhibited significantly higher relative post-challenge survival (65.52%) against Aeromonas hydrophila infection. Collectively, these results suggest that dietary supplements of ginger (at 0.8%) can promote growth performance, skin mucus immune parameters, and strengthen immunity of L. rohita. Therefore, ginger represents a promising food additive for carps in aquaculture. (C) 2016 Elsevier Ltd. All rights reserved.OAIID:RECH_ACHV_DSTSH_NO:T201604968RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:3.148DEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo rohita macrophages via the suppression of the NF-kappa B signalling pathway

Pinocembrin is a flavonoid that has been reported to exhibit various pharmacological and biological activities including antimicrobial, antioxidant, and anti-inflammatory. To explore the anti-inflammatory activity of pinocembrin in a fish cell line, we investigated its ability to regulate the inflammatory mediators elevated by lipopolysaccharide (LPS) in Labeo rohita head-kidney (HK) macrophages. HK macrophages of L. rohita were treated with LPS (1 (mu g mL(-1)) in the presence or absence of pinocembrin. We examined the inhibitory effect of pinocembrin on LPS-induced nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production. The inhibitory effect of pinocembrin on nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was investigated by RT-PCR and western blot. The effect of pinocembrin on pro-inflammatory cytokines (tumour necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta)) and anti-inflammatory cytokine IL-10 was investigated by ELISA and RT-PCR. The phosphorylation of three mitogen activated protein kinases (MAPKs) ERK, JNK, and p38 was analysed by western blot. Pinocembrin inhibited LPS-induced productions of NO and PGE2, and also markedly inhibited TNF-alpha, IL-1 beta, iNOS, and COX-2 production in a concentration-dependent manner. In addition, TNF-alpha and IL-1 beta mRNA expression levels decreased significantly, while IL-10 mRNA expression increased (P < 0.05) with pinocembrin pre-treatment. RT-PCR and western blot analysis showed that pinocembrin decreased both the mRNA and protein expression levels of LPS-induced iNOS and COX-2 in HK macrophages. Pinocembrin suppressed the phosphorylation of MAPK in LPS-stimulated HK macrophages. Further, pinocembrin significantly inhibited LPS-induced NF-kappa B transcriptional activity via the attenuation of I kappa B alpha degradation. Taken together, pinocembrin reduced the levels of pro-inflammatory mediators, such as iNOS, COX-2, TNF-alpha, and IL-1 beta, by inhibiting NF-kappa B activation via the suppression of ERK and p38 phosphorylation, and by attenuating the degradation of I kappa B alpha. These results suggest that pinocembrin is a potential novel candidate for the treatment of inflammatory conditions in L. rohita macrophages. (C) 2016 Elsevier Ltd. All rights reserved.OAIID:RECH_ACHV_DSTSH_NO:T201600579RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:3.148DEPT_NM:수의학과EMAIL:[email protected]_YN:YCONFIRM:

Draft Genome Sequence of a Clinical Isolate, Aeromonas hydrophila SNUFPC-A8, from a Moribund Cherry Salmon (Oncorhynchus masou masou)

OAIID:RECH_ACHV_DSTSH_NO:220130000030777004RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076079CITE_RATE:0FILENAME:Genome Announc.-2013-Han-.pdfDEPT_NM:수의학과EMAIL:[email protected]_YN:NFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/c8016102-dfe3-43b1-8df0-05d68b13741f/linkCONFIRM: