14 research outputs found
Molecular Identification of Korean Polychaetes Based on Mitochondrial COI Gene Sequence
Polychaetes are one of the dominant organisms in marine benthic ecosystem, yet understanding of their taxonomy and genetics is limited. Most of the polychaete species have trouble in identification of their species based on the morphological characters due to their similarity among the species. In this study, we attempted a genetic identification of Korean Polychaetes using a partial sequence (658bp) of the mitochondrial gene, cytochrome c oxidase subunit I (COI), which is widely used as a DNA barcode marker. A total of 22 families were included in the analysis: Onuphidae , Capitellidae , Maldanidae , Cirratulidae , Dorviileidae , Lumbrineridae , Oenonidae , Onuphidae , Scalibregmidae , Glyceridae , Lacydoniidae , Nephtyidae , Nereididae , Phyllodocidae , Pilargidae , Polynoidae , Sabellidae , Poecilochaetidae , Spionidae , Eunicidae , Terebellidae , Trichobranchidae. The sequence comparison shows that high variability of the COI sequence within and between the species. Neighbour-Joining tree suggested a sister relationship between Lacydoniidae and phyllodocidae. Further analysis based on more comprehensive species sampling and sequence information would provide a more robust validation of phylogenetic relationships among the polychaete species, genera and families.2
Molecular Identification of Korean Polychaetes Based on Mitochondrial COI Gene Sequence
Polychaetes are one of the dominant organisms in marine benthic ecosystem, yet understanding of their taxonomy and genetics is limited. Most of the polychaete species have trouble in identification of their species based on the morphological characters due to their similarity among the species. In this study, we attempted a genetic identification of Korean Polychaetes using a partial sequence (658bp) of the mitochondrial gene, cytochrome c oxidase subunit I (COI), which is widely used as a DNA barcode marker. A total of 22 families were included in the analysis: Onuphidae , Capitellidae , Maldanidae , Cirratulidae , Dorviileidae , Lumbrineridae , Oenonidae , Onuphidae , Scalibregmidae , Glyceridae , Lacydoniidae , Nephtyidae , Nereididae , Phyllodocidae , Pilargidae , Polynoidae , Sabellidae , Poecilochaetidae , Spionidae , Eunicidae , Terebellidae , Trichobranchidae. The sequence comparison shows that high variability of the COI sequence within and between the species. Neighbour-Joining tree suggested a sister relationship between Lacydoniidae and phyllodocidae. Further analysis based on more comprehensive species sampling and sequence information would provide a more robust validation of phylogenetic relationships among the polychaete species, genera and families.2
Paleoenvironment of the Last Glacial Age and Thanatochoenoses from surface sediment of the Southeastern Continental Shelf, Korea
한국 남동 대륙붕 표층퇴적물에서 산출하는 패각유해군집 특성과 최종빙하기 고환경을 파악하기 위하여 총 26개 정점에서 시료를 채취, 종 분류, 군집분석 및 14C 연대측정을 행하였다. 패류유해 총 개체수 13,074개에서 11속 170종(이매패강 73속 105종, 권패강 43속 60종, 굴족강 3속 5종)이 분류되었으며, GVPA, GPPA, GCA, GVCA, PPA, OLA, DA, NCA, LGA, PASA, GpA 및 BA 등 총 12개 군집이 설정되었다. 패류유해군집 중 11개 정점에서 나타나는 GVPA, GPPA, GCA, GVCA 군집은 Glycymeris Fauna로서 쓰시마를 중심으로한 남동 대륙붕 일대에 분포하며 약 6,000년전 형성된 것으로 생각된다. 그리고 약 15,000년전 서식한 조간대∼천해성종 Scapharca broughtonii, Macoma calcrea, Callithaca adamsi 및 Mya truncata의 서식심도 분석 결과 남해대륙붕은 현 해수면 보다 수심이 약 150∼160m 낮았던 것으로 나타났다. 또한 제주도 후기 플라이오세 서귀포 동물군의 대표적인 화석종인 Mizuhopecten tokyoensis hokurikuensis, Mizuhopecten yessoensis 산출은 최종빙하기 최저해수면시기에 후기 플라이오세 퇴적층의 노출 또는 현재 대륙붕 해저면에 후기플라이오세 퇴적층이 노출되어 있을 가능성을 지시하는 것으로 생각된다.
This paper deals with the classification of species, assemblage analysis, and 14C age dating about molluscan collected from total 26 stations in the southeastern continental shelf of Korea. The total individual number of 13,074 thanatochoenoses were classified into 11 genus 170 species (Bivalvia: 73genus 105species, Gastropoda: 43 genus 60species, Scaphopoda: 3 genus 5 species), and 12 following assemblages, GVPA, GPPA, GCA, GVCA, PPA, OLA, DA, NCA, LGA, PASA, GPA, and BA, are fixed. Among the molluscan assemblages, GVPA, GPPA, GCA, GVCA assemblages occurred at 11 stations found to be the Glycymeris Fauna. It was observed that Glycymeris Fauna are distributed mainly on Tsushima Island around south continental shelf of Korea. And it can be concluded based on 14C age dating that they were occurred about 6,000 years ago. Scapharca broughtonii, Macoma calcrea, Callithaca adamsi and Mya truncata that inhabited in intertidal∼neritic areas around 15,000 yrs B.P. show that south continental shelf of Korea was approximately 150∼160 meters lower than the present sea-level. In additiont, the production of Mizuhopecten tokyoensis hokurikuensis, Mizuhopecten yessoensis seem to indicate that the Late Piocene shell bed outcropped during the LGM or in the present sea-level of the southeastern continental shelf of Korea.22Nkc
The early impacts of the ‘Hebei Spirit’ oil spill on the Macrozoobenthic Communities in the subtidal area around Tae-an, western coast of Korea
본 연구는 충남 태안에서 발생한 허베이 스피리트 유류유출 사고 직후 유류오염이 대형저서동물 군집에 미치는 초기 영향을 알아보기 위해 사고, 10일 후인 2007년 12윌 18일부터 22일까지 72개 정점을 대상으로 조사를 실시하였다. 사고 주변 해역에서 출현한 대형저서동물은 총 228종, 서식밀도는 561 개체 m−2 였으며, 생물량은 39.7 gww m−2 였다. 본 연구에서 가장 많은 개체수를 보인 대형저서동물은 단각류의 Ampelisca sp.였다. 그러나 이 종은 유류 오염에 민감한 종으로 유류오염의 직접적 영향을 받았던 만리포 주변 해역에서는 전혀 출현하지 않았다. 반면 기회주의적 다모류에 속하는 얼굴갯지렁이류의 Prionospio bocki, Prionospio cirrifera가 만리포 주변 해역에서 우점하게 나타나는 등 유류오염에 의한 징후가 나타났다. 또한 유류사고 직전에 수행된 연구 결과들에 비해 서식밀도 및 생물량 조성이 크게 감소하였다. 그러나 일부 해사채취해역에 위치한 정점들에서는 유류사고 전후의 군집조성이 큰 차이를 보이지 않았다.
In order to detect the early impacts of the Hebei Spirit oil spill on the subtidal macrozoobenthos, we conducted a benthic survey at 72 stations in the subtidal area of Tae-an, the eastern Yellow Sea from Dec. 18th 2007 to Dec. 22th after 10 days from the Hebei Spirit oil spill. A total of228 species from 72 stations was sampled with mean density of 561 ind m−2 and mean biomass of 39.7 gww m−2 . In this study, Ampelisca occurred as a major dominant fauna in this survey, but did not appear around oil spill areas. Meanwhile some opportunistic polychaete worms like Prionospio bocki, Prionospio cirrifera appeared in the oil spill area, especially near coastal beaches. The overall abundance and biomass of macrozoobenthos decreased after oil spill. However some stations previously disturbed with sand extraction showed a similar macrozoobenthic community structure.22Nkc
The DNA barcode, molecular characters for Korean Polychaetes.
Polychaetes appear as dominant organisms in many of the coastal marine ecosystems, yet understanding of their taxonomy and genetics is limited. Most of the polychaete species have trouble in identification of their species based on the morphological characters due to their similarity among the species. On the other hand, the molecular markers such as the DNA barcode provide objective characters distinguishing each species apart. In Korea, polychaetes inhabit a variety of habitats in all the coasts. Because the mud flats has been well-developed, polychaetes have a great diversity and abundance. In this study, we present the DNA barcode, the mitochondrial gene, cytochrome c oxidase subunit I (COI) as the molecular characters for Korean Polychaetes in order to establish an objective references for species identification. Polychaetes were collected by grap sampling in the southern sea of Korea, identified morphologically, and imaged digitally. DNA was extracted, PCR amplified, and the standard barcode region was sequenced bidirectionally. A total of 48 individuals belonging to 33 species in 29 genera were analyzed and their identities were affirmed by both morphology and molecular characters. The species include Heteromastus filiformis, Clymenella koreana, Praxillella affinis, Maldane cristata, Scalibregma inflatum, Dorvillea annulata, Ninoe palmate, Arabella iricolor, Diopatra bilobata, Glycera chirori, Glycera subaenea, paralacydonia paradoxa, Nephtys ciliate, Nectoneanthes latipoda, Perinereis nuntia, Perinereis aibuhitensis, Nereis multignatha, Nectoneanthes oxypoda, Anaitides koreana, Genetyllis castanea, Eulaia viridi, Anaitides maculate, Sigambra tentaculata, Lepidonotus tenuisetosus, Chone teres, Poecilochaetus johnsoni, Laonice cirrata, Amaeana trilobata, Loimia medusa, Terebellides horikoshii, Melinna cristata, Aphelochaeta manitaris, Cirriforania tentaculata.2
Genetic Identification of Korean Polychaetes collected from Chuja and Jeju island, using the DNA Barcode
A total of 63 individuals belonging to 33 species in 8 order of polychaetes collected from Chuja and Jeju island were sequenced for a 658bp partial region of the mitochondrial cytochrome c oxidase subunit I(COI) gene, the typical molecular marker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful momarker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful mo2
Genetic Identification of Korean Polychaetes collected from Chuja and Jeju island, using the DNA Barcode
A total of 63 individuals belonging to 33 species in 8 order of polychaetes collected from Chuja and Jeju island were sequenced for a 658bp partial region of the mitochondrial cytochrome c oxidase subunit I(COI) gene, the typical molecular marker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful momarker called DNA barcode. The species include Nicomache minor, Maldane pigmentata, Praxillella affinis of the order Capitellida, Dorvillea annulata, Protodorvillea kefersteini, Marphysa bellii, Lumbrineris japonica, Lumbrineris latreilli, Marphysa mortenseni, Diopatra bilobata of the order Eunicida, Pherusa parmata of the order Flabelligerida, Armandia lanceolata, Scalibregma inflatum of the order Opheliida, Glycera chirori, Glycera onomichiensis, Micropodarke dubia, Nephtys oligobranchia, Nephtys ciliata, Paralacydonia paradoxa, Eteone longa, Sigambra tentaculata, Syllis spongiphila, Trypanosyllis taeniaformis, Aglaophamus sinensis, Inermonephtys japonica, Anaitides koreana of the order Phllodocida, Owenia fusiformis, Chone teres of the order Sabellida, Laonice cirrata of the order Spionida, Ampharete arctica, Amphicteis gunneri, Melinna cristata, Terebellides horikoshii of the order Terebellida. Average intraspecific and interspecific distances based on Kimura two parameter model(K2P) were 3.5% and 34.9% respectively. The Neighbor-joining tree for all 63 sequences revealed the phylogenetic relationship among the species. The result supports the role of the COI gene as useful mo2
