바이오분자 검출을 위한 유전율 변화 전계 효과 트랜지스터 어레이와 범용 판독 플랫폼의 개발

학위논문(박사) - 한국과학기술원 : 전기및전자공학전공, 2009. 8., [ xi, 160 p. ]In this thesis, general-purpose readout platforms are studied in detection of various biomolecules with dielectric modulated field effect transistor (DMFET) array for point-of-care testing (POCT) applications. For preliminary study on field effect transistor (FET)-based electrical biosensors, a DNA sensor array is fabricated and characterized with an integrated current readout circuitry. The operational principle of the fabricated DNA sensor is based on the ion sensitive field effect transistor (ISFET) and the intrinsic charge of DNA affects the surface potential of the sensing region in the sensors. Various concentrations of polymerase chain reaction (PCR) amplicon are successfully quantified with the implemented readout system. As a first version of DMFET array in this research, a single-gate dielectric modulated field effect transistor (SGDMFET) array is fabricated and characterized with a custom designed readout platform. A prostate cancer marker, prostate specific antigen (PSA), and its antibody are immobilized in the vertical nanogap of SGDMFET. With the implemented readout platform, drain currents of SGDMFET array are monitored and successfully detect PSA and antibody by current increments caused by dielectric constant changes. Finally, a double-gate dielectric modulated field effect transistor (DGDMFET) array is fabricated and characterized with a revised version of readout platform. In order to verify nanogap filling with sample solution and evaporation of the sample solution from the nanogap, calculations and simulations are performed. Antigen and antibody of avian influenza are successfully detected by threshold voltage shifts caused by dielectric constant changes in the nanogap. In order to avoid cross-contamination, fabricated biosensor arrays of SGDMFET and DGDMFET are mounted on discrete PCBs as cartridges to be used in the application of POCT. Implemented system can be used as a general-purpose readout platform to detect various kinds of...한국과학기술원 : 전기및전자공학전공

COATING LAYER

본 발명은 소수성, 소유성, 발수성 및 발유성을 갖는 코팅막에 관한 것이다.본 발명의 실시 예에 따른 코팅막은 기판과 상기 기판의 표면에서 바깥쪽으로 돌출된 돌출부를 포함하되, 상기 돌출부는 상기 기판의 표면과 수직한 방향으로의 단면이 역사다리꼴이고, 상기 돌출부는 상기 기판의 표면에 복수로 배열되되, 서로 인접하는 두 돌출부들은 서로 이격되도록 배열된 것을 특징으로 한다