Distinct spatial localization of three types of phosphatidyl choline in rat buccal mucosa identified by matrix-assisted laser desorption/ionization imaging mass spectrometry

浜松医科大学博士（医学）doctoral医学系研究科The buccal mucosa is an inner lining exposed to frequent friction caused by teeth occlusion and therefore, a vulnerable site to lesions such as stomatitis, leukoplakia, lichen planus and cancer. Although it is considered an important tissue in the field of oral surgery, not enough information regarding the buccal mucosa is available. In this study, we characterized the buccal mucosa in the oral cavity using imaging mass spectrometry (IMS). Tissues such as epithelium, lamina propria and muscle were identified in rat buccal mucosa by HE staining. Moreover, IMS data of high-intensity ions were classified into 4 groups according to their distribution. In a previous IMS analysis of mouse tongue tissue, we detected linoleic acid-containing phosphatidyl choline (PC)(diacyl 16:0/18:2), oleic acid-containing PC(diacyl 16:0/18:1) and DHA-containing PC(diacyl 16:0/22:6) as major PCs. In the present work, we analyzed buccal mucosa tissue with emphasis on the aforementioned PC by IMS and showed that the investigated PCs existed in the layers of epithelium, lamina propria, and muscle at different ratios. This is the first study to analyze by IMS the buccal mucosa. The results presented here are likely to provide the perspective on understanding oral environments and to develop treatments for oral disorders.doctoral thesi

視覚障害者向けのCAPTCHAに関する研究

明治大学博士（数理科学）Article2017doctoral thesi

Electronic and structural effects on the 13C contact shifts of σ-bonded molecules

Carbon-13 n.m.r. contact shifts induced by nickel acetylacetonate have been observed for various nitrogen heterocyclic compounds in order to elucidate the manner of stereospecific electron spin transmission through the σ-bonded skeleton. It is revealed that the substituent at the α-carbon atom has a sensitive effect on the mode of the carbon-13 contact shifts. The experimental trend is discussed in relation to changes in the electronic structure of ligands or in metal-ligand bonding caused by the introduction of substituents. It is also found that hybridization of the nitrogen non-bonded orbital in ligand molecules plays an important role in intramolecular electron spin transmission. These results are discussed with the aid of molecular orbital calculations of electron spin densities.application/pdfjournal articl

Urbanization in Narashino-Shi viewd from the Population phenomena

短報Short Articlesdepartmental bulletin pape

Research on Learning System made of Student’s Ability in Technology Education.

text紀要論文 / Departmental Bulletin Paperdepartmental bulletin pape

A Survey of Khünkhüree Sites in Mongolia and Their Significance : A Basic Study on the “Konggulie granary” of the Yuan Dynasty

モンゴル高原は，そのすべてが遊牧に適した水や草の豊かな地帯ではない。 険しい山地や乾燥地が広範囲にみられ，その中に遊牧可能な小地域がパッチ状 に点在しているのが実像である。モンゴル高原に成立した歴代の遊牧国家の版 図は，一見すると広大だが，じつはこのような小地域の集合体と考えた方がよ かろう。遊牧だけでなく，農耕や手工業も，このような限られた生活空間でお こなわれていた。一つ一つの小地域が自立あるいは他地域の欠落点を補完しな がら，有機的に結合することによって，遊牧国家の領域が成り立っていたと考 える。そうであるならば，それぞれの小地域内の自然環境や生産力，歴史的変 遷などを知ることで，遊牧国家の興亡の背景の一端が明らかになるはずだ。 　このような視点に立って筆者らは，モンゴル国ウブルハンガイ県フンフレー 地区で調査をおこなった。そこはゴビ地帯にあるオアシスで，13 ～ 14 世紀の 遺跡があり，文献史料にも登場する。考古学，歴史学，地理学などが協力して 学際的にアプローチできる，格好の地域といえる。 　その結果，この地域はモンゴル帝国時代には「孔古烈」とよばれ，豊富な湧 水を利用して，モンゴル高原の中心地カラコルムへ食糧を供給した農耕地帯で あったことがわかった。あわせて，この地はゴビ砂漠の南北縦断路と，アルタ イ山脈北麓を通るモンゴル高原の東西横断路の交点で，交通の要衝であるとと もに，軍事的攻防の舞台であった。これらのことから，今後モンゴル帝国の興 亡史を研究する上で重要な地域になると指摘した。Not all areas of the Mongol plateau are rich in water and grass, vital for nomads. Steep mountainous regions and arid terrain stretching for miles are dotted with small parcels of land where nomads can survive. The territory of the successive generations of nomadic states which established themselves on the Mongol plateau may appear vast at first glance, but it should really be considered as a collection of these small parcels. Not only nomadism, but also farming and cottage industries were carried on in these restricted living spaces. Probably the nomadic states were formed by organic bonding among small areas as individual parcels became self-reliant or could provide what another parcel lacked. If this is the case, then by analyzing aspects such as the natural environment, productive strength and historical transformation within each individual parcel, we should be able to throw light on the background to the rise and fall of the nomadic states. From this perspective, the authors investigated the Khünkhüree district in Mongolia’s Övörkhangai Prefecture. In an oasis in the Gobi region, there are remains from the 13th–14th centuries which are also mentioned in written records. This area is well-suited to an interdisciplinary approach by archaeology, historical study and geography. As a result, we discovered that the area was called Konggulie during the period of the Mongol empire and was a farming area, utilizing abundant underground water, which supplied provisions to Kharakhorum in the center of the Mongol plateau. The area lay at the crossroads between the route which cut through the Gobi desert from north to south and the route which ran eastwest across the Mongol plateau past the northern foothills of the Altai mountain range. We show that as well as being a strategic point for traffic, the fact that it was a stage for military attack and defense makes it an important area for future research into the history of the rise and fall of the Mongol empire.departmental bulletin pape

ラジオ波焼灼術後の安静による苦痛の緩和を目的とした体位変換の取り組み －患者にアンケート調査を行って－

departmental bulletin pape

Measurement of neutrino oscillation by the K2K experiment

journal articl

Wobbling excitation of triaxial nuclear molecule 28

journal articl

RNAi efficiency in different tissues in <i>rrf-1</i> mutants.

<p>‘+’ and ‘−’ refer to the presence or absence of a phenotype in response to RNAi treatment in wild-type or <i>rrf-1</i> animals. The <i>rrf-1(pk1417), rrf-1(pk1417).4x (rrf-1(pk1417) four times outcrossed to WT(A)</i> and <i>rrf-1(ok589)</i> mutants behaved similarly to RNAi targeting endogenous genes and are combined here as <i>rrf-1</i>. For strains expressing GFP-tagged transgenes, loss of GFP expression was assayed in response to <i>gfp</i> RNAi in the <i>rrf-1(pk1417)</i> mutant only.</p><p>Phenotype abbreviations: Pro: proximal germ cell proliferation abnormal; Tum: tumorous germline; Prz: paralyzed, Unc/Twitcher: uncoordinated, sub-class, Twitcher; Gob: gut-obstructed; Clr: clear; Lva: larval arrest; Gro: slow growth; Ste: sterile; Bli: blistered; Dpy: dumpy; Bmd: body morphology defects; DTC: distal tip cell.</p>1<p>Citations refer to studies describing expression patterns and gene functions of the genes targeted by RNAi and to descriptions of the transgenes used;</p>2<p>Only the intestinal expression of <i>daf-16p::gfp::daf-16</i> was examined;</p>3<p>The intestinal, pharyngeal, and gonadal expression of <i>lgg-1p::gfp::lgg-1</i> was examined;</p>4<p>Data not shown;</p>5<p>RNAi phenotypes observed in 2^nd generation;</p>6<p>Transgene expression was only partially reduced.</p