<Original Article> Age-Related Trend in Biological Rhythms Investigated Via a Periodic-Linear Regression Analysis: the Clinospectror Method

This report deals with a biostatistical procedure for analyzing the trend of biological rhythms as a function of age. The method is based on a periodic-linear regression analysis (PLRA) in which the periodic time of biological rhythms interacs with the linear time of chronological age. The procedure, called ""clinospectror method"", analyzes the trend for the properties of biological rhythms, i.e., the mean level of oscillation, the extent of waveform profile, and the timing of oscillatory crest. The clinospectror method allows to detect specific models of aging processes in bioperiodic functions in virtue of the concordance or discordance in the age-related trend for rhythmometric parameters. The PLRA also allows to forecast past and future properties, as well as decay times, in bioperiodic phenomena. Finally, the method is applicable to the assessment of biological age, and to the compution of senility index.journal articl

「進め 日本語教室」 第1回 「日本語教師1年生レポート」

一般雑誌記事 / Articlearticl

How the Knowledge System of Product Design Varies with Era

In order to research the variation of knowledge system of Product Design with era, I selected three books which were published in 1960s, 1980s and 2000s in Japan. From these books I dig out many terms of knowledge, and assort them into three categories, (1) the common terms among three books, (2) two books and (3) one term in one book. (1) means the basic knowledge of Product Design for a long term, such as a general idea, history, form and function. (3) means the new terms of the era. These results will be useful for making the curriculum of Product Design in the near future.departmental bulletin pape

Broad Band Software-Defined Radio Receivers Based on Superconductive Devices

We have studied software-defined radios (SDRs) based on superconducting devices. The increasing data rate in mobile communications will require a band-width of up to a few hundred MHz in the future. We have confirmed numerically the broad-band characteristic of oversampled analog-to-digital converters including a lowpass and bandpass modulator. Furthermore, the bandpass modulator constructed without a resistor is found to have sensitively improved by two orders of magnitude compared to that of the lowpass modulator. This result means that the digital RF radio architecture based on a bandpass ADC is better suited for a future SDR receiver.journal articl

Acquiring and Characterizing Plane-to-Ray Indirect Light Transport

Separation of light transport into direct and indirect paths has enabled new visualizations of light in everyday scenes. However, indirect light itself contains a variety of components from subsurface scattering to diffuse and specular interreflections, all of which contribute to complex visual appearance. In this paper, we present a new imaging technique that captures and analyzes these components of indirect light via light transport between epipolar planes of illumination and rays of received light. This plane-to-ray light transport is captured using a rectified projector-camera system where we vary the offset between projector and camera rows (implemented as synchronization delay) as well as the exposure of each camera row. The resulting delay-exposure stack of images can capture live short and long-range indirect light transport, disambiguate subsurface scattering, diffuse and specular interreflections, and distinguish materials according to their subsurface scattering properties.conference pape

FUS CLIP-seq identified increased FUS binding in introns flanking cassette exons.

<p>A) FUS CLIP assay conditions. B) Western blot analysis of FUS protein immunoprecipitated from FUS CLIP. C) Autoradiography of radiolabeled FUS-RNA complexes from FUS CLIP. Red boxes highlight FUS protein complex regions selected for further analysis. D) Percentage of FUS CLIP clusters mapped to the indicated regions (UTR: untranslated region; CDS: coding sequence; ncRNA: non-coding RNA). E) Normalized complexity map of FUS binding at 87 FUS-associated cassette exons and flanking introns. Control was an average of 100 sets of normalized complexity of 87 constitutive exons randomly selected from genes expressed in HeLa cells, as determined by RNA-seq.</p

ALS-Associated <i>FUS</i> Mutations Result in Compromised <i>FUS</i> Alternative Splicing and Autoregulation

<div><p>The gene encoding a DNA/RNA binding protein <i>FUS/TLS</i> is frequently mutated in amyotrophic lateral sclerosis (ALS). Mutations commonly affect its carboxy-terminal nuclear localization signal, resulting in varying deficiencies of FUS nuclear localization and abnormal cytoplasmic accumulation. Increasing evidence suggests deficiencies in FUS nuclear function may contribute to neuron degeneration. Here we report a novel FUS autoregulatory mechanism and its deficiency in ALS-associated mutants. Using FUS CLIP-seq, we identified significant FUS binding to a highly conserved region of exon 7 and the flanking introns of its own pre-mRNAs. We demonstrated that FUS is a repressor of exon 7 splicing and that the exon 7-skipped splice variant is subject to nonsense-mediated decay (NMD). Overexpression of <i>FUS</i> led to the repression of exon 7 splicing and a reduction of endogenous FUS protein. Conversely, the repression of exon 7 was reduced by knockdown of FUS protein, and moreover, it was rescued by expression of EGFP-FUS. This dynamic regulation of alternative splicing describes a novel mechanism of FUS autoregulation. Given that ALS-associated FUS mutants are deficient in nuclear localization, we examined whether cells expressing these mutants would be deficient in repressing exon 7 splicing. We showed that FUS harbouring R521G, R522G or ΔExon15 mutation (minor, moderate or severe cytoplasmic localization, respectively) directly correlated with respectively increasing deficiencies in both exon 7 repression and autoregulation of its own protein levels. These data suggest that compromised FUS autoregulation can directly exacerbate the pathogenic accumulation of cytoplasmic FUS protein in ALS. We showed that exon 7 skipping can be induced by antisense oligonucleotides targeting its flanking splice sites, indicating the potential to alleviate abnormal cytoplasmic FUS accumulation in ALS. Taken together, FUS autoregulation by alternative splicing provides insight into a molecular mechanism by which FUS-regulated pre-mRNA processing can impact a significant number of targets important to neurodegeneration.</p></div

Data_Sheet_1_RELN gene-related drug-resistant epilepsy with periventricular nodular heterotopia treated with radiofrequency thermocoagulation: a case report.pdf

An increasing number of gene mutations associated with epilepsy have been identified, some linked to gray matter heterotopia—a common cause of drug-resistant epilepsy. Current research suggests that gene mutation-associated epilepsy should not be considered a contraindication for surgery in epilepsy patients. At present, stereoelectroencephalography-guided radiofrequency thermocoagulation is an important method to treat periventricular nodular heterotopia-associated drug-resistant epilepsy. We present a case of drug-resistant epilepsy, accompanied by periventricular nodular heterotopia and a heterozygous mutation of the RELN gene, successfully treated with radiofrequency thermocoagulation, resulting in a favorable outcome.</p