Diffuse transport and spin accumulation in a Rashba two-dimensional electron gas

The Rashba Hamiltonian describes the splitting of the conduction band as a result of spin-orbit coupling in the presence of an asymmetric confinement potential and is commonly used to model the electronic structure of confined narrow-gap semiconductors. Due to the mixing of spin states some care has to be exercised in the calculation of transport properties. We derive the diffusive conductance tensor for a disordered two dimensional electron gas with spin-orbit interaction and show that the applied bias induces a spin accumulation, but that the electric current is not spin polarized.journal articl

第10号

一般雑誌記事 / Articlearticl

ゲンチョ ノウソッチュウ エキハッショウ シゼン コウケツアツ ラット ニ オケル アンジオテンシン II 1ガタ ジュヨウタイ キッコウヤク CV 11974 ノ シンキン ホゴ コウカ ニツイテ

【目的】アンジオテンシンIIの左室拡張能と左室の線維化,心肥大との関連を明らかにするために,脳卒中易発症自然高血圧ラット(SHRSP)を用い検討した。【方法】SHRSPに12週齢から24週齢まで,アンジオテンシンII 1型受容体拮抗薬 CV-11974を投与(0.5mg/kg i.p.)した治療群,ヒドララジンを投与(14-27mg/kg p.o.)した治療群および未治療群と正常血圧コントロールとしてのWKY群で収縮期血圧,心体重比,心筋細胞面積,間質の線維化,冠血管の中膜の厚さ,心筋 stiffness,反応充血時の最大冠灌流量,最小血管抵抗,ミオシン重鎖V3の割合を計測し比較検討した。【結果】CV-11974には心肥大抑制効果があるがヒドララジンでは認められなかった。しかし,降圧により間質の線維化は CV-11974とヒドララジンでは共に抑制が認められたが,冠血管周囲の線維化および中膜の肥厚の抑制効果は CV-11974にしか認められなかった。同様に,心筋 stiffness,冠予備能,さらにはミオシン重鎖の比率においても CV-11974にしか正常化させる効果がなかった。【結論】アンジオテンシンIIは心筋の phenotype changeや線維化を誘導する主要な因子であり,拡張能障害に深く関与していると考えられた。journal articl

Improved measurement of CP-violation parameters sin⁡2ϕ1 and |λ|, B meson lifetimes, and B0-B̅0 mixing parameter Δmd

journal articl

マダム花子のdeath scenes について

departmental bulletin pape

Protein Thermal Stability Enhancement by Designing Salt Bridges: A Combined Computational and Experimental Study

<div><p>Protein thermal stability is an important factor considered in medical and industrial applications. Many structural characteristics related to protein thermal stability have been elucidated, and increasing salt bridges is considered as one of the most efficient strategies to increase protein thermal stability. However, the accurate simulation of salt bridges remains difficult. In this study, a novel method for salt-bridge design was proposed based on the statistical analysis of 10,556 surface salt bridges on 6,493 X-ray protein structures. These salt bridges were first categorized based on pairing residues, secondary structure locations, and Cα–Cα distances. Pairing preferences generalized from statistical analysis were used to construct a salt-bridge pair index and utilized in a weighted electrostatic attraction model to find the effective pairings for designing salt bridges. The model was also coupled with B-factor, weighted contact number, relative solvent accessibility, and conservation prescreening to determine the residues appropriate for the thermal adaptive design of salt bridges. According to our method, eight putative salt-bridges were designed on a mesophilic β-glucosidase and 24 variants were constructed to verify the predictions. Six putative salt-bridges leaded to the increase of the enzyme thermal stability. A significant increase in melting temperature of 8.8, 4.8, 3.7, 1.3, 1.2, and 0.7°C of the putative salt-bridges N437K–D49, E96R–D28, E96K–D28, S440K–E70, T231K–D388, and Q277E–D282 was detected, respectively. Reversing the polarity of T231K–D388 to T231D–D388K resulted in a further increase in melting temperatures by 3.6°C, which may be caused by the transformation of an intra-subunit electrostatic interaction into an inter-subunit one depending on the local environment. The combination of the thermostable variants (N437K, E96R, T231D and D388K) generated a melting temperature increase of 15.7°C. Thus, this study demonstrated a novel method for the thermal adaptive design of salt bridges through inference of suitable positions and substitutions.</p></div

The primer sequences for generating various ABO alleles cDNA.

<p>The primer sequences for generating various ABO alleles cDNA.</p

Δ<i>T</i>_m and kinetic parameters of wild-type BglA and mutant enzymes.

a<p><i>T</i>_m of wild-type protein was detected to be 38.5°C.</p>b<p>Suggested mutation.</p><p>n.d.: Not determined.</p><p>Δ<i>T</i>_m and kinetic parameters of wild-type BglA and mutant enzymes.</p

The frequency for the alternative splicing types.

<p>The frequency for the alternative splicing types.</p

High-Resolution Melting Molecular Signatures for Rapid Identification of Human Papillomavirus Genotypes

<div><h3>Background</h3><p>Genotyping of human papillomarvirus (HPV) is crucial for patient management in a clinical setting. This study accesses the combined use of broad-range real-time PCR and high-resolution melting (HRM) analysis for rapid identification of HPV genotypes.</p> <h3>Methods</h3><p>Genomic DNA sequences of 8 high-risk genotypes (HPV16/18/39/45/52/56/58/68) were subject to bioinformatic analysis to select for appropriate PCR amplicon. Asymmetric broad-range real-time PCR in the presence of HRM dye and two unlabeled probes specific to HPV16 and 18 was employed to generate HRM molecular signatures for HPV genotyping. The method was validated via assessment of 119 clinical HPV isolates.</p> <h3>Results</h3><p>A DNA fragment within the L1 region was selected as the PCR amplicon ranging from 215–221 bp for different HPV genotypes. Each genotype displayed a distinct HRM molecular signature with minimal inter-assay variability. According to the HRM molecular signatures, HPV genotypes can be determined with one PCR within 3 h from the time of viral DNA isolation. In the validation assay, a 91% accuracy rate was achieved when the genotypes were in the database. Concomitantly, the HRM molecular signatures for additional 6 low-risk genotypes were established.</p> <h3>Conclusions</h3><p>This assay provides a novel approach for HPV genotyping in a rapid and cost-effective manner.</p> </div