Studies on Electrophoresis of Erythrocyte : Part 1. Measuremnent of Red Cell Mobility in Various Diseases

Article信州医学雑誌 16(5): 897-904(1967)journal articl

Improved measurements of the partial rate asymmetry in B→hh decays

journal articl

Space Charge Dynamics in Polypropylene

We investigated the space charge dynamics in polypropylene, M-PP and Z-PP which were polymerized using metallocene and Ziegler catalysts, respectively. Space charge distributions depended upon temperature, polarity and electrode materials. Carriers injected from the semiconducting electrode played an important role in the formation of space charge in PP at high temperatures. The amount of space charge was the largest around 40℃ for both M-PP and Z-PP. They showed similar space charge dynamics in spite of their difference in morphology. Z-PP showed larger current than M-PP. These results were also compared with those of polyethylene and they were discussed considering the difference in morphology among M-PP, Z-PP and polyethylene.journal articl

知的財産権を用いた資金提供・調達に関する実態調査の結果と今後のあり方について

departmental bulletin pape

Loss of the micronuclear C3ΔRFB rDNA allele during vegetative propagation

<p><b>Copyright information:</b></p><p>Taken from "Deletion of the rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome"</p><p>Nucleic Acids Research 2006;34(2):620-634.</p><p>Published online 30 Jan 2006</p><p>PMCID:PMC1356531.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Heterozygous germline transformants (C3ΔRFB/B or wild-type C3/B) were continually propagated following the establishment of clonal lines (transformant clones 1–13). Expanded clones were serially passaged by transferring ∼1000 cells into fresh media (1:100 dilution). DNA was isolated at ∼70 (early) and ∼150 (late) fissions and subjected to PCR amplification with primers that selectively amplify micronuclear C3 rDNA (wild-type C3 or C3ΔRFB alleles, primers 2 + 36), or wild-type C3 and B rDNA (primers 2 + 23). Control PCR templates—B: wild-type B rDNA strain (CU428), C: wild-type C3 rDNA strain (SB1934). The micronuclear genotype of clonal TX611 lines could not be unambiguously established due to the potential presence of macronuclear rDNA minichromosomes derived from aberrant rDNA excision ( and , C3-long)

Abnormal transition from conjugal development to the first vegetative cell division in heterozygous C3ΔRFB/B progeny

<p><b>Copyright information:</b></p><p>Taken from "Deletion of the rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome"</p><p>Nucleic Acids Research 2006;34(2):620-634.</p><p>Published online 30 Jan 2006</p><p>PMCID:PMC1356531.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Normal kinetics for pair formation and separation in C3ΔRFB mutant progeny. Microscopic analysis of pair formation and separation in wild-type crosses [SB210 × TX614(1)], crosses between wild-type and mutant strains [SB210 × TX607(1), SB210 × TX610(11), SB210 × TX611(1)] and cross between two mutant strains [TX607(1) × TX610(11)]. () Cytological examination of crosses between wild-type strain SB210 and wild-type C3 rDNA transformant, TX614(1) (WT × WT),or SB210 and C3ΔRFB transformant, TX607(1) (WT × Mutant) with the DNA staining dye apofluor. The vast majority of cells displayed normal progression through development (data not shown), with a small percentage of mutant mating partners containing extra micronuclei/pronuclei prior to genetic exchange (compare micrographs 1 and 6). Panels 2–5 (WT × WT) and 7 ×10 (WT × Mutant) depict representative cells in exconjugant mating populations (24 h mating followed by 7–9 h re-feeding). () Cartoon depicting the progression of mating cells during development (0–24 h) and the fate of exconjugants after re-feeding at 24 h for (WT × WT), and (WT × mutant) or (mutant × mutant) crosses. See text for details. () Quantification of the number of ‘abnormal cells’ for different mating 7–9 h after re-feeding (see text for details). () Progeny of crosses between TX611(1) and A* mating type III obtained by single pair isolation after 23–25 h and subsequent propagation for 5–10 passages. Note the appearance of extra nuclei in the mutant (micrographs 2–4) compared with wild-type (micrograph 1). Staining: apofluor (micrographs 1–3), DAPI (micrograph 4)

Heterozygous C3ΔRFB/B strains undergo stochastic excision of the rDNA in the developing macronucleus

<p><b>Copyright information:</b></p><p>Taken from "Deletion of the rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome"</p><p>Nucleic Acids Research 2006;34(2):620-634.</p><p>Published online 30 Jan 2006</p><p>PMCID:PMC1356531.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Restriction map and fragment sizes for products following digestion with EcoRV (downward arrowheads) or XbaI (upward arrowheads) for micronuclear rDNA locus (Mic), excised non-palindromic macronuclear rDNA (NP) and palindromic (P) rDNA minichromosomes derived from wild-type B rDNA (WT) or C3ΔRFB deletion alleles. Hybridization probes A and B: thick black lines in micronuclear rDNA diagram. () Stochastic appearance and structure of macronuclear C3 rDNA in clonal C3ΔRFB transformants. Left panel: Southern blot analysis of EcoRV digested genomic DNA with probe B. Wild-type B rDNA strain; CU428, heterozygous C3ΔRFB/B germline transformant strains; TX607(1), TX610(11) and TX611(1), and wild-type C3 rDNA transformant strain; TX614(1). The position of wild-type B and wild-type C3 rDNA monomers (WT non-pal) and palindromes (WT pal), predicted C3ΔRFB species (ΔRFB pal, ΔRFB non-pal), and unexpected products (C3-long and X) are indicated. The corresponding restriction fragments for expected products are symbol coded (see panel A). TX611(C1) and TX611(C3) are clonal progeny derived from a cross between TX611(1) and amicronucleate strains A* III and A* V, respectively, and have generated a new macronucleus by round II genomic exclusion (see text). Note the presence of the C3ΔRFB rDNA palindrome in TX611(C1) and absence of this species in its parent, TX611(1). Right panel: Southern blot analysis of genomic DNA with probe B from mating progeny harvested 24 h after mixing strains of opposite mating type. Crosses: TX611(C1) or TX611(C3) were mated with tester strains, CU427 (wild B rDNA in micronucleus) or SB1934 (wild-type C3 rDNA in micronucleus). Control mating: CU427 × SB1934. X indicates rDNA species that were not predicted by conventional processing at the Cbs element upstream of the rDNA 5′ NTS. () The C3-long rDNA species is fragmented at a novel site upstream of the 5′ Cbs element. Southern blot of XbaI digested genomic DNA hybridized with probe B (left) or probe A (right). Numbers correspond to clonal lines established from each heterozygous C3ΔRFB/B rDNA germline transformant

曲げ試験による木材のせん断強さ測定について

application/pdfSince the chair-type specimen in use as JIS shearing specimen has a sharp right-angled notch, the shearing plane of this specimen undergoes an influence of stress concentration and couple of forces. Consequently, shearing strength of this specimen is very small in comparison with true shearing strength. Accordingly, taking note of the bending test that gives pure horizontal shearing stress, the experiment of the measurement ofshearing strength was carried out by the bending test by means of the reinforced Hinoki specimen with soft steel plates in ordcr to prevent bending fracture. The results obtained are summarized as follows: 1. The test for the measurement of shearing strength of eleven kinds of specimens with various beam height and span length was carried out by the bending test. The specimen with beam height of 29 mm and span-height ratio of 6 was most suitable for the measurement of shearing strength of Hinoki. 2. As a result of measurement of shearing strength of the reinforced specimens with soft steel plate, JIS specimens and RADCLIFFE'S specimens, shearing strength of the reinforced specimen was larger than that of JIS specimen and was smaller than that of RADCLIFFE'S specimen. 3. On the assumption that the state of elasticity went on until the fracture, shearing strength of the reinforced Hinoki specimen was 122 ± 14 kg/cm² in L-T specimen and was 102±10Kg/cm² in L-R specimen.departmental bulletin pape

Examination about pattern making program for apparel

In sewing, a (paper) pattern is the paper or cardboard templates from which the parts of a garment are traced onto fabric before cutting out and assembling. In recent years, it has increased to perform the design of such a pattern by CAD software. However, in general, it is difficult to arrange a pattern efficiently. This problem is called two-dimensional packing problem. In this paper, we deal with algorithm for two-dimensional apparel pattern making, and show the first basic program.departmental bulletin pape

Emmanuelis Alvarez Pegas ... Commentaria ad ordinationes Regni Portugalliae tractatio scientifica, utrique foro perutilis, ac necessaria, ex Iure naturali, Ecclesiastico, Civili, Romano, Hispano & Lusitano ; tomus sextus : in quo agitur de ardilium materia, officio, et jurisdictione, obligationeque & de quibus causis cognoscat vel non ; de officialibus mechanias & eorum utilitate & taxa ...

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