ゲンチョ キョセイ ラット オ モチイタ ゼンリツセン ハツガン ニオケル テストステロン ノ プロモーション サヨウ ノ ケントウ

F344系雄性ラットを用いて,プロモーション期にある前立腺に対して testosteroneの投与が前立腺癌の発生,進展に及ほす影響を検討した。11週齢で全ラットを去勢し,これをA～Dの4群に分けた。去勢後3週間でA群,B群は testosterone propionate (TP) 25mg (平均体重227.5±21g)を3日間連続皮下投与して前立腺上皮の増殖を促し,1日おいてN-methyl-N-nitrosourea (MNU) 10mgを静脈内に投与した。さらにA群はMNU投与2日後より56週にわたり testosterone enanthate (TE) 12.5 mgを4週間に1度皮下に投与した。C群はTPおよびMNUは投与せず,A群と同期間TEを投与した。D群は去勢のみとした。60週で全群屠殺解剖し,病理組織学的に比較検討した。付属生殖器癌はA群では26匹中4匹(15%):前立腺癌3匹,精癌1匹に認めた。しかし,他の3群には付属生殖器癌は認めなかった。付属生殖器増殖性病変(癌+過形成)はA群では7/26(27%),C群では1/8(12%)に認めたが,B群,D群ではそれぞれ24匹,9匹のいずれにも認めなかった(A群,B群間にp<0.05で有意差あり)。プロモーション期のTE投与は付属生殖器増殖性病変に促進的に働くと思われた。またA,B両群には皮膚扁平上皮癌をそれぞれ16/26(62%),13/24(54%)にさらに小腸の腺癌をそれぞれ8/26(31%),4/24 (17%)に認めた。journal articl

Improved measurements of the partial rate asymmetry in B→hh decays

journal articl

On the Water Sorption of Starch Granules

application/pdf澱粉粒における水分収着や結晶化度と収着水分量あるいは収着熱について述べた。澱粉での単分子収着水分量は7～8%であって、飽和水分状態ではグルコース残基あたり6分子の水分収着と思われる。澱粉の結晶化度はリゲイン法で31～45%である。X線回折法による澱粉の結晶化度は収着水分量の増加にともなって増大し、飽和水分状態ではリゲイン法や比容積法による値と同程度になると思われる。澱粉の結晶化熱はジャガイモ、カンショ、タピオカでグルコース残基あたり0.4～0.5kcalで、トウモロコシ、コムギでは1.9～2.2kcalであった。正味積分収着熱からサツマイモ、タピオカ澱粉の非晶部分はトウモロコシ、コムギの1.2倍であった。departmental bulletin pape

Leveraging IoT and Weather Conditions to Estimate the Riders Waiting for the Bus Transit on Campus

The communication technology revolution in this era has increased the use of smartphones in the world of transportation. In this paper, we propose to leverage IoT device data, capturing passengers' smartphones' Wi-Fi data in conjunction with weather conditions to predict the expected number of passengers waiting at a bus stop at a specific time using deep learning models. Our study collected data from the transit bus system at James Madison University (JMU) in Virginia, USA. This paper studies the correlation between the number of passengers waiting at bus stops and weather conditions. Empirically, an experiment with several bus stops in JMU, was utilized to confirm a high precision level. We compared our Deep Neural Network (DNN) model against two baseline models: Linear Regression (LR) and a Wide Neural Network (WNN). The gap between the baseline models and DNN was 35% and 14% better Mean Squared Error (MSE) scores for predictions in favor of the DNN compared to LR and WNN, respectively.conference pape

ANALYSES OF FLOW FIELD STRUCTURES AROUND LINEAR-TYPE AEROSPIKE NOZZLES USING LIF AND PSP

Aerospike nozzles have been expected as a candidate for an engine of reusable Space Shuttles to respond to growing demand for rocket-launching at the lower cost. In this study, the flow field structures in any cross sections around the linear-type aerospike nozzle are visualized and analyzed, using laser induced fluorescence (LIF) of NO seeded in the carrier gas N/sub 2/. Since the flow field structure is affected mainly by the pressure ratio (P/sub s//P/sub a/), the linear-type aerospike nozzle is set inside the vacuum chamber to carry out the experiments in the wide range of pressure ratios from 75 to 250. Flow fields are visualized in several cross-sections, demonstrating the complicated three-dimensional flow field structures. Pressure sensitive paint (PSP) of PtTFPP bound by poly(TMSP) is also applied successfully to measurement of the complicated pressure distribution on the spike surface.journal articl

Complete catalogue of lncRNA in genome-wide promoters across multiple human cancer cells

Promoter associated noncoding transcripts derived from 41 GRO-Seq datasets of human cancer cell lines in different conditions.Only non-overlapping promoters are considered. Sense and antisense transcripts relative to adjacent gene direction are reported.</div

PPARβ/δ activation modulates gene expression.

<p>(<b>A</b>) H358, H441 and A549 cells were grown to confluence, starved for 24 h and then treated with GW501516 (5 µM) for 18 h. Total RNA was isolated and examined by RT-PCR. (<b>B</b>) H358 cells were incubated with GW501516 for the indicated times prior to RNA extraction and analysis.</p

PI3K/Akt activation by PPARβ/δ agonists.

<p>(<b>A</b>) H358 cells were grown to confluence, starved for 24 hours and then incubated for 2 h with LY294002 (25 µM) or wortmannin (200 nM) followed by GW501516 (5 µM) for 18 h. RNA was extracted and analyzed by RT-PCR. (<b>B</b>) H358 and H441 cells were incubated with and without GW501516 for the indicated time. Cell lysates were examined by immunoblotting with antibodies for total and phosphorylated Akt, PTEN and p85α. (<b>C</b>) H358 cells were transfected with His-PPARβ/δ or empty pcDNA3.1 vector and incubated with and without GW501516 for 18 h. Cells were lysed in RIPA buffer and His-PPARβ/δ was pulled down with His-select nickel affinity gel. Immunoblots were developed with antibodies for PPARβ/δ and p85α. (<b>D</b>) H358 cells were transfected with pcDNA3.1, full length His-PPARβ/δ (PPARβ/δ 1–441), or truncated PPARβ/δ retaining the N-terminal part (PPARβ/δ 1–168) and C-terminal part (PPARβ/δ 168–441), lysed in RIPA buffer and subject to immunoprecipitation with anti-p85α antibody. Immunoblots of whole cell lysates and immunoprecipitates (<i>middle and bottom panel</i>, respectively) were performed with antibodies directed to p85α, tubulin and His-tag. Arrows indicate full length and truncated PPARβ/δ detected in immunoblots of whole cell lysates and immunoprecipitates. <i>Top panel</i>, schematic representation of PPARβ/δ structure and domain organization.</p

Transcriptional and Non-Transcriptional Functions of PPARβ/δ in Non-Small Cell Lung Cancer

<div><p>Peroxisome proliferator-activated receptor β/δ (PPARβ/δ) is a nuclear receptor involved in regulation of lipid and glucose metabolism, wound healing and inflammation. PPARβ/δ has been associated also with cancer. Here we investigated the expression of PPARβ/δ and components of the prostaglandin biosynthetic pathway in non-small cell lung cancer (NSCLC). We found increased expression of PPARβ/δ, Cox-2, cPLA₂, PGES and VEGF in human NSCLC compared to normal lung. In NSCLC cell lines PPARβ/δ activation increased proliferation and survival, while PPARβ/δ knock-down reduced viability and increased apoptosis. PPARβ/δ agonists induced Cox-2 and VEGF transcription, suggesting the existence of feed-forward loops promoting cell survival, inflammation and angiogenesis. These effects were seen only in high PPARβ/δ expressing cells, while low expressing cells were less or not affected. The effects were also abolished by PPARβ/δ knock-down or incubation with a PPARβ/δ antagonist. Induction of VEGF was due to both binding of PPARβ/δ to the VEGF promoter and PI3K activation through a non-genomic mechanism. We found that PPARβ/δ interacted with the PI3K regulatory subunit p85α leading to PI3K activation and Akt phosphorylation. Collectively, these data indicate that PPARβ/δ might be a central element in lung carcinogenesis controlling multiple pathways and representing a potential target for NSCLC treatment.</p> </div

PPARβ/δ knock-down and antagonist prevent VEGF induction.

<p>(<b>A</b>) H358 and H441 cells were transfected with PPARβ/δ siRNA and control GL3 and then treated with GW501516 (5 µM) for 18 h. Gene levels were determined by RT-PCR. (<b>B</b>) Cells were incubated for 2 h with GSK0660 (10 µM) and then with GW501516 (5 µM) for 18 h prior analysis by RT-PCR. (<b>C</b>) H358 cells were treated with GW501516 for 18 h and processed for chromatin immunoprecipitation using anti-PPARβ/δ and anti-IgG antibodies. The region of the VEGF promoter containing a PPRE (−527/−298) and a non-targeted region (−1338/−1123) were PCR amplified. The PPRE containing region of the ADRP promoter was amplified as positive control. <i>Top panel</i>, representative gel scan. <i>Bottom panel</i>, densitometric quantification of three independent experiments in H441 cells. *P<0.01.</p