12 research outputs found
<Original Article> Typennachweis des Faktors H in Blutspuren
By means of isoelectric focusing and immunoblotting the possibility of the determination of HF polymorphism was investigated on dried bloodstains under various storage conditions. The three common phenotypes, HF A, HF AB and HF B, were reliably demonstrable at 37℃ for up to 4 weeks, at room temperature for up to 7 weeks and at 4℃ for over 10 weeks. The results showed that this complement system can provide a quite useful, new means for the identification of unknown stored bloodstains.journal articl
3RD HARMONIC OPERATION OF SIT INVERTER RF SOURCE FOR ICRF HEATING IN THE DIVERTOR PLASMA SIMULATOR NAGDIS-II
The purpose of this paper is to study the 3rd harmonic operation of a voltage source inverter power supply. A Static Induction Transistor (SIT) inverter power supply is used in this experiment. This power supply can generate 14 kW in steady state operation and its upper limit frequency of the gate driver is 1.7 MHz. The advantage of the 3rd harmonic operation is that the RF power at higher frequency range beyond the switching limit can be generated. It should be also noted that the driver circuit needs no special modification for this operation. The conversion efficiency and maximum output power are investigated as a function of output frequency. The conversion efficiency with harmonic operation is a little lower than the conversion efficiency with fundamental operation. At the frequency of <3 MHz, the conversion efficiency is higher than that of conventional linear amplifiers when using this SIT inverter power supply.journal articl
Hype Live: Biometric-based Sensory Feedback for Improving the Sense of Unity in VR Live Performance
We propose Hype Live, a system to improve the sense of unity by sharing responses through visual, auditory, and haptic stimuli based on the biometrics of the participants in VR live performances. In this field, the sharing of reactions among the participants is one of the most important factors in improving the sense of unity. However, not many past studies have provided feedback to the participants' senses. Therefore, as a prototype of Hype Live, we used a vibration device to provide haptic feedback and recreated moshing scenes where the participants violently collide with each other like a real live performance.conference pape
Imagem da virtude em o noviciado da Companhia de Jesus na corte de Lisboa .../
Mode of access: Internet.Port. a dos tintas con grab. xilográfico del anagrama de la Compañía de Jesús.Sign: *-2*4, A-Z4, 2A-2Z4, 3A-3Z4, 4A-4Z4, 5A-5Z4, 6A-6G4, 6H2
Pietra del paragone politico : tratta dal monte Parnaso : doue si toccano i gouerni delle maggiori monarchie dell'Uniuerso /
Mode of access: Internet.El lugar de impresión es falso, el ICCU da Venecia como probable lugar.Sign.: A-N
Phenotypic characterization of cell lines.
<p>A. Expression of BRCA1 was analysed by Western blotting using anti-BRCA1 antibody. Mouse anti-BRCA1 primary antibody was purchased from Oncogene Research (San Diego, CA). Actin was used as a loading control. B. Proliferation experiments were performed using the Sulforhodamine B technique. Data are means of three independent experiments + SEM. C. Cycle distribution was determined using flow cytometry. Data are means of three independent experiments + SEM. D. Percentage of apoptotic cells was determined at 48 h using the Annexin V test. Data are presented as means of 3 independent replicates + SEM. *: p<0.05.</p
BRCA1-induced increased activity of antioxidation pathways.
<p>A. The major enzymes and metabolites involved in antioxidation metabolism were quantified by q-RT-PCR, <sup>1</sup>H-NMR and by LC-MS. Data are presented as means of at least 3 independent replicates + SEM. *: p<0.05; **: p<0.01, SUM1315-BRCA1 vs SUM1315-LXSN cell lines. B. Schematic representation of glutathione metabolism and its regulation by BRCA1. Genes (<i>italicized</i>) in red: overexpression. Metabolites: grey, not detected; red, increased.</p
Regulation of metabolism by BRCA1 protein in SUM1315-LXSN and SUM1315-BRCA1 cell model.
<p>A. Gene expression study: mRNA of SUM1315-LXSN and SUM1315-BRCA1 cells were extracted and analyzed on TaqMan Low Density Arrays as described in materials and methods. The replicates represent independent culture experiments. Low levels of expression are represented in blue and high levels of expression in yellow. B. The major metabolites observed by <sup>1</sup>H-NMR and by LC-MS were analyzed by hierarchical clustering<b>.</b> The replicates represent independent culture experiments. Small quantities of metabolites are represented in blue and high quantities in yellow.</p
Role of BRCA1 in energetic metabolism.
<p>A. The major enzymes and metabolites involved in energy metabolism were quantified by q-RT-PCR, <sup>1</sup>H-NMR and by LC-MS. Data are presented as means of at least 3 independent replicates + SEM. *: p<0.05; **: p<0.01, +: p = 0.06 for lactate, SUM1315-BRCA1 vs SUM1315-LXSN cell lines. B. Analyses of HK2 and IDH1 enzymes was performed by Western Blot and quantified by ImageJ software. Data are presented as means of 3 independent replicates + SEM. *: p<0.05; ***: p<0.001, SUM1315-BRCA1 vs SUM1315-LXSN cell lines. C. The oxygen partial pressure was measured in the culture medium of SUM1315-LXSN and SUM1315-BRCA1 cell lines subjected to hypoxic atmosphere for the indicated time. *: p<0.05, SUM1315-BRCA1 vs SUM1315-LXSN cell lines. D. Production of lactate and consumption of glucose were measured in cell culture media by enzymatic colorimetric assays. Data are presented as means of 3 independent replicates + SEM.</p
