Observation of B0→D*-(5π)+, B+→D*-(4π)++ and B+→D̅*0 (5π)+

journal articl

Communication Model of Design Education based on Research into the Creative Thinking Process

The aim of this research is to examine the knowledge acquisition process in design education. In this research, the author indicated a tentative assumption for constructing a 'Learning - Teaching Communication' Model in order to build up the educational programs for the students of the Design faculty, Tsukuba College of Technology (TCT). A unique feature of this research is that it is trying to understand the communication process between educators and students by replacing into co-creative process models. After the consideration, which was based on the results of the empirical studies, a model of the co-creativity in design education was proposed as the outcomes, which was modified and layered on the general model of creative design thinking process. From this model, it became evidently become clear that using metaphors would stimulate the students' creativity in the early stage of the design process.departmental bulletin pape

Research on Creativity in Design Education On the role of expression and reflection upon the process

The purpose of this research is to understand the creativity aimed at in design education. This research was progressed by the method of verifying educational practice of Visual Communication Design for the purpose. The lesson of the Visual Communication Design currently practiced for hearing impaired students, in Faculty of Design, Tsukuba College of Technology was made into the example. It inferred what kind of creativity the student demonstrated from the example. Consequently, it turned out that the student raised the creativity when they connected with their self-expression. In addition, it also turned out that self-reflection of a creation process is effective in design process. The result of this research shows that future design education needs to take hold on expression in the individual inner world more.departmental bulletin pape

Effect of Anti-Oxidants on Space Charge in Low-Density Polyethylene

journal articl

Data for: Synthetic biology-inspired design of signal-amplifying materials systems

File contains the raw/processed data required to reproduce the findings in the above-identified wor

Site-directed mutagenesis with AQUA Cloning: deletion-, insertion- and point-mutagenesis.

<p><b>(a)</b> For targeted deletion of the Lck membrane anchor from an Lck-fused EGFP, a primer pair was designed for whole-plasmid PCR with the forward primer annealing to EGFP and sharing overlaps to the P_SV40 promoter region. The reverse primer anneals to the P_SV40 promoter and shares overlaps to EGFP. The resulting PCR product hence excludes the Lck membrane anchor and the extremes are complementary by 32 bp allowing AQUA assembly into a circular plasmid for cytosolic expression of EGFP. The resulting EGFP expression plasmid was further used as a PCR template with a primer pair where the 5’-extensions were replaced with a mitochondrial target signal (MTS) which localized expressed EGFP into the mitochondria of the host cell. The flanking MTS sequence of the resulting PCR product serves as region of homology for AQUA Cloning to construct the expression plasmid with the MTS sequence inserted. <b>(b)</b> Confocal imaging after transient transfection into NIH/3T3 cells reveals successful AQUA Cloning: the deletion of the Lck-membrane anchor resulted in cytosolic expression of EGFP (middle) of previously membrane localized protein (left), and the insertion of a MTS in recruitment of EGFP into the mitochondria (right). Scale bars, 10 μm. Corresponding fluorescence intensity profiles are given below each figure. <b>(c)</b> For site-directed substitution of a single amino acid in EGFP, a primer pair was designed surrounding the target site. 5’-extensions were attached to encode the Y66H substitution (leading to blue fluorescence) and at the same time contributing a region of homology for AQUA Cloning of a single linear PCR product. <b>(d)</b> Green and blue fluorescence of recombinant purified EGFP and EGFP-Y66H, respectively upon excitation with 366 nm light. <b>(e)</b> Excitation and emission spectra of EGFP and EGFP-Y66H.</p

Additional file 2: Table S1. of A synthetic mammalian network to compute population borders based on engineered reciprocal cell-cell communication

Expression vectors and oligonucleotides designed and used in this study. (DOCX 35 kb

Orthogonal Optogenetic Triple-Gene Control in Mammalian Cells

Optogenetic gene switches allow gene expression control at an unprecedented spatiotemporal resolution. Recently, light-responsive transgene expression systems that are activated by UV-B, blue, or red light have been developed. These systems perform well on their own, but their integration into genetic networks has been hampered by the overlapping absorbance spectra of the photoreceptors. We identified a lack of orthogonality between UV-B and blue light-controlled gene expression as the bottleneck and employed a model-based approach that identified the need for a blue light-responsive gene switch that is insensitive to low-intensity light. Based on this prediction, we developed a blue light-responsive and rapidly reversible expression system. Finally, we employed this expression system to demonstrate orthogonality between UV-B, blue, and red/far-red light-responsive gene switches in a single mammalian cell culture. We expect this approach to enable the spatiotemporal control of gene networks and to expand the applications of optogenetics in synthetic biology

Screen for AQUA Cloning efficiency among common lab-strains of <i>E</i>. <i>coli</i>.

<p>The cloning of the 2-DNA fragment assembly (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137652#pone.0137652.g002" target="_blank">Fig 2</a>) was performed at room temperature with 32 bp of homology and with DNA produced by PCR. Different commercially available chemically competent strains were tested along the TOP10 cells prepared in our lab. The efficiency was determined by analytical colony PCR of 12 clones (BL21 (DE3), 9 clones), see Fig B in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137652#pone.0137652.s001" target="_blank">S1 File</a>.</p

Combinatorial AQUA Cloning for plant cells.

<p>(a) For a combinatorial cloning of 3 plasmids, each originating from 3 linear DNA parts (variants of a ratiometric auxin sensor) out of a pool of 5 parts, were constructed. The consensus sequences derived from the Aux/IAA 31 and Aux/IAA 17 proteins, or an auxin insensitive sequence, each fused to the firefly luciferase were prepared. The fragments were cloned together with the renilla luciferase, separated by a 2A peptide for equimolar expression, into a 35S promoter-driven plant expression vector. Expression of the sensor constructs in plant cells should enable the auxin-dependent degradation of firefly, while renilla remains unaffected for intrinsic signal normalization. <b>(b)</b> Relative firefly:renilla ratios obtained from protoplasts expressing the sensor variants after 1 h of auxin treatment. Error bars represent standard error of the mean, n = 6.</p