Observation of B0→D*-(5π)+, B+→D*-(4π)++ and B+→D̅*0 (5π)+

journal articl

Generation and application of wavelength tunable ultrashort soliton pulse in optical fiber

0.78-1.0 μm wavelength tunable femtosecond soliton pulse generation using photonic crystal fibers and fiber laser is demonstrated. Novel pulse trapping phenomena by soliton pulse are discovered and 1 THz ultrafast all optical switching is demonstrated.journal articl

Cytological Studies of Early Stages of Powdery Mildew in Barley and Wheat (X) : Fluorescence at Penetration Sites of Fungi on Barley, Wheat and Rice

application/pdf前報で，オオムギうどんこ病菌（Erysiphe graminis hordei）の分生胞子がオオムギ子葉鞘細胞に進入する際に，侵 入点を中心にして円状に緑色螢光が発生することを報告した。この現象が，オオムギとE.graminis hordeiの系に特 異的なものであるかどうかを検討するために，オオムギ・コムギの子葉鞘およびイネ葉鞘に，E.graminis hordei,Erysiphe graminis tritici（コムギうどんこ病菌), Alternaeia kikuchiana（ナシ黒斑病菌）の分生胞子を接種し，それぞれの菌の 付着器下に蛍光が発生するか否かを観察した。オオムギ子葉鞘上では，すべての供試菌の付着器または付着器様構造 下で螢光が認められた。コムギの子葉鞘上のE.graminis hordeiの侵入点には弱い蛍光が現われた。しかし，イネ葉 鞘に同じ菌を接種しても侵入点付近に蛍光は現われなかった。以上の結果から，螢光の発生は種々の糸状菌の侵入に 対するオオムギ・コムギの子葉鞘の共通的な反応であると結論されるが，螢光の強さは植物によって差があり，オオムギ子葉鞘では強い蛍光が発生するようである。departmental bulletin pape

Industrial Tribunals in Great Britain

研究ノートapplication/pdfdepartmental bulletin pape

Iulii Caesaris Ruginelli I.C. Medionalensis Practicarum Quaestionum Rerumque indicatarum liber singularis ...

Colofón, texto a dos columnas.Mode of access: Internet.Portada a dos tintas con grab. xil., colofón con grab. xil., viñetas xil

The <i>smpB-ssrA</i> Mutation Affects Survival of Mice Infected with <i>Y. pseudotuberculosis</i>

<div><p>(A) Thirty mice, in groups of five mice per strain in three independent experiments, were challenged via the orogastric route with 2 × 10⁹ CFU of wild-type (WT) or ΔBA strain.</p><p>(B) For complementation studies, a total of 16 mice, in groups of four mice per strain in two independent experiments, were infected via the orogastric route with wild-type, ΔBA (p<i>smpBssrA</i>), or ΔBA (pBR322<i>)</i> strains. Mice were monitored for at least 21 d post bacterial infection.</p></div

The ΔBA Mutant Is Defective in Survival and Replication within Macrophages

<p>The J774A.1 cell line was infected with an equal number of 28 °C–grown log-phase wild-type (WT) or ΔBA strain of <i>Y. pseudotuberculosis</i>. Extracellular bacteria were removed and intracellular bacteria were enumerated at 1 h and 25 h post infection as described in Materials and Methods. Error bars indicate standard deviations of triplicate samples.</p

The ΔBA Mutant Is Defective in Secretion of Yops

<div><p>Bacterial cultures of indicated strains of <i>Y. pseudotuberculosis</i> were grown in low-calcium medium, and cells were harvested 3.5 h after temperature shift to 37 °C. Culture supernatants were precipitated with TCA, and the protein pellets were resolved by electrophoresis on 10%-polyacrylamide Tris-tricine gel, and stained with Coomassie Brilliant Blue. The Δ<i>yopE</i> strain was used as a reference.</p><p>MW stds, protein molecular weight standards; WT, wild type.</p></div

Wild-Type and ΔBA Strains Display Different Patterns of Tissue Colonization

<p>Groups of seven mice per strain were infected via the orogastric route with 2 × 10⁹ cells and sacrificed on day 4 of infection. Harvested tissues were processed for CFU determination as described in Materials and Methods. Each symbol represents the CFU contained in the indicated tissue sample from one mouse. Data were analyzed by Student <i>t-</i>test in order to determine statistical significance of CFU counts in Peyer's Patches (<i>p</i> < 0.051), MLN (<i>p</i> < 0.271), and spleen (<i>p</i> < 0.044). The <i>smpB-ssrA</i> mutant was found to be able to reach extra-intestinal sites but unable to proliferate efficiently within the MLN and spleen.</p

Affect of <i>smpB-ssrA</i> Mutation on VirF and YmoA Protein Levels

<p>Cultures of wild-type (WT) or ΔBA strains of <i>Y. pseudotuberculosis</i> were grown in secretion non-permissive (lanes 1 and 2) or permissive medium (lanes 3 and 4) at 37 °C for 3.5 h as described in Materials and Methods. Bacteria were harvested and analyzed for VirF (A) and YmoA (B) proteins by Western blot analysis using specific polyclonal antibodies. Purified YmoA protein was used as a control (lane 5).</p