<Original Article> Determination of TF Subtypes in Human Organ Tissues Using Isoelectric Focusing and Immunoblotting

Transferrin (TF) subtyping in various human organ tissues (spleen, pancreas, heart,liver, muscle, lung, kidney, skin, brain, prostate, testis, ovary and uterus) was carried out using isoelectric focusing and immunoblotting. TF subtypes were demonstrated in fresh tissue samples of almost all the organs examined except a few samples of the pancreas and skin. In tissue samples of the heart, muscle, lung, kidney, brain, prostate, testis, ovary and uterus that were stored at room temperature, TF subtyping was possible for up to 1 week. The findings showed that the TF subtype system is useful for medicolegal individualization of human tissues.journal articl

Preparation and guest binding of novel β-cyclodextrin dimers linked with various sulfur-containing linker moieties

Novel cyclodextrin dimers (1, 2, 3 and 4) whose cyclodextrins were linked with sulfur-containing linkers, namely a thiodipropanamide, a dithiodipropanamide, a thiodiethanamide, or a dithiodiethanamide linker, were synthesized by a reaction of 6-amino-6-deoxycycIoheptaose 7 with the corresponding dicarboxylic acids. For their preparation, dicyclohexylcarbodiimide, l-(3-dimethylaminopropyl)-3-ethylcarbodiimideand(benzotriazol-l-yloxy) tripyrroIidinophosphonium hexafluorophosphate were examined as coupling reagents. 'H NMR studies of the dimers suggested an intramolecular inclusion of the linker moiety to the cyclodextrin cavity, which affected the complexation of guest molecules. Dimer 4 was converted to another type of dimer 5 by reductive cleavage of the disulfide bond to generate thiol group-containing mononieric species followed by a substitution reaction with 6-O-tosyl derivative 6.application/pdfjournal articl

Observation of B0→D*-(5π)+, B+→D*-(4π)++ and B+→D̅*0 (5π)+

journal articl

条約法に関するウィーン条約第60条に関する一考察(2) : 歴史的変容、多数国間条約への適用および５項の意義

departmental bulletin pape

Space Charge and Field Distributions in Low-Density Polyethylene

The space charge distributions in LDPE (low-density polyethylene) were measured by PEA (pulsed electro-acoustic) method. We used two kinds of LDPEs: m-LDPE polymerized with metallocene catalyst and LDPE polymerized under high-pressure process. The space charge in these two samples strongly depended upon the material of electrode. Semicon electrode enhanced carrier injection into LDPEs. The measurements of space charge dynamics revealed the effective mobility of positive and negative carriers. The space charge was much more stable in m-LDPE than in LDPE.journal articl

Feasibility Study of Magnetism-based Indoor Positioning Methods in an Incineration Plant

In an incineration plant, remote operation from a centralized control room is now possible, but inspection and cleaning of equipment still require a worker to visit the site. When the plant owner reduces the number of workers due to operation costs, it will be standard for a single worker to visit the site. Therefore, it is necessary to monitor the location of workers in real-time to detect unexpected human accidents quickly. Conventional methods use radio waves, such as Wi-Fi and Bluetooth, but there is little demand for communication equipment in the incineration plant. However, there is not enough demand for communication facilities in the incineration plant. It is too large to bear the cost of installing wireless access points, and Bluetooth Low Energy (BLE) beacons just for positioning. Therefore, we are focusing on magnetism using for indoor positioning method. In addition, the incineration plant has a lot of types of equipment that contains a wide range of magnetized metals, large motors, and generators. We could observe the magnetic peculiarity at each point. Based on these assumptions, we have developed a new indoor positioning method at the incineration plant. This paper describes the development of an indoor positioning system for an incineration plant. And we propose three methods for fingerprinting matching: Point matching, Path matching, and DTW matching. The average positioning errors of these methods are 6.89 m, 0.05 m, and 0.06 m, respectively.conference pape

The Right Back Leg of the Woolly Mammoth<i>(M. primigenius)</i> Found in Siberia

<p>The well-preserved mammoth body fragment with foot (33 × 36 cm), shin, and ankle-joint (the total length is ~88 cm) was found in the Enmynveem River valley (north-eastern Siberia, Chukotka). The tissue material (bones, muscles, and skin) had no visible marks of tissue damage by insects or other animals. Radiocarbon dating of the skin and muscle tissue determined that the mammoth lived 32,850 ± 900 y ago [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040073#pbio-0040073-b012" target="_blank">12</a>].</p

Mitochondrial Genome of the Woolly MammothM. primigenius

<p>The complete mitochondrial genome was determined independently in two different laboratories using designs with multiple primers for overlapping PCR fragments ranged from ~325 to ~650 bp; the longer PCR fragments were also produced and sequenced. The overlapped PCR products used for sequencing and cloning are shown by the inner circle. Only PCR fragments produced from different pairs of primers are shown. Two genes,<i>ATP6</i> and<i>ND4L,</i> overlap with neighboring genes.</p

Paenungulata Tree and Phylogenic Relationship of the Woolly Mammoth

<p>The analysis of complete mtDNA sequences placesM. primigenius withE. maximus on the tree. The Sirenia<i>(D. dugon)</i> and Hyracoidea<i>(P. capensis),</i> most closely related species among extant taxa to Elephantinae, were taken as outgroups. Bootstrap values and posterior probabilities were calculated using a Bayesian approach [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040073#pbio-0040073-b029" target="_blank">29</a>,<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040073#pbio-0040073-b031" target="_blank">31</a>] assuming a gamma distribution of the rates of evolution across sites with a General Time Reversible model (normal font), HKY model (bold), with a parsimony approach (italic), and by neighbor joining (italic and bold) [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040073#pbio-0040073-b028" target="_blank">28</a>]. The scale is 0.1 substitutions per site. The mitochondrial genomes of<i>M. primigenius, E. maximus A, E. maximus B, L. africana A, L. africana B, D. dugon,</i> andP. capensis were used in the analysis.</p

The Unusually Well-Preserved Mammoth DNA

<p>(A) Nuclei with DNA clearly detectable by DAPI staining in muscle cells of ~33,000-y-oldM. primigenius. (B) Total genomic DNA isolated from the mammoth muscle tissue (lane 1 is 1/10 dilution of the DNA on lane 2); control DNA from fresh human blood samples in lanes 3 and 4. (C) Examples of PCR products (~300–600 bp) for mammoth mitochondrial genome. (D) PCR amplification recovers long sequences for complete mitochondrial genes (1,317 bp<i>CytB</i> and 1,613 bp<i>ATP6</i> genes), but PCR of larger fragments (3,054 bp<i>ND5</i>) is failed.</p