<Original Article> Determination of TF Subtypes in Human Organ Tissues Using Isoelectric Focusing and Immunoblotting

Transferrin (TF) subtyping in various human organ tissues (spleen, pancreas, heart,liver, muscle, lung, kidney, skin, brain, prostate, testis, ovary and uterus) was carried out using isoelectric focusing and immunoblotting. TF subtypes were demonstrated in fresh tissue samples of almost all the organs examined except a few samples of the pancreas and skin. In tissue samples of the heart, muscle, lung, kidney, brain, prostate, testis, ovary and uterus that were stored at room temperature, TF subtyping was possible for up to 1 week. The findings showed that the TF subtype system is useful for medicolegal individualization of human tissues.journal articl

Preparation and guest binding of novel β-cyclodextrin dimers linked with various sulfur-containing linker moieties

Novel cyclodextrin dimers (1, 2, 3 and 4) whose cyclodextrins were linked with sulfur-containing linkers, namely a thiodipropanamide, a dithiodipropanamide, a thiodiethanamide, or a dithiodiethanamide linker, were synthesized by a reaction of 6-amino-6-deoxycycIoheptaose 7 with the corresponding dicarboxylic acids. For their preparation, dicyclohexylcarbodiimide, l-(3-dimethylaminopropyl)-3-ethylcarbodiimideand(benzotriazol-l-yloxy) tripyrroIidinophosphonium hexafluorophosphate were examined as coupling reagents. 'H NMR studies of the dimers suggested an intramolecular inclusion of the linker moiety to the cyclodextrin cavity, which affected the complexation of guest molecules. Dimer 4 was converted to another type of dimer 5 by reductive cleavage of the disulfide bond to generate thiol group-containing mononieric species followed by a substitution reaction with 6-O-tosyl derivative 6.application/pdfjournal articl

Observation of B0→D*-(5π)+, B+→D*-(4π)++ and B+→D̅*0 (5π)+

journal articl

Space Charge and Field Distributions in Low-Density Polyethylene

The space charge distributions in LDPE (low-density polyethylene) were measured by PEA (pulsed electro-acoustic) method. We used two kinds of LDPEs: m-LDPE polymerized with metallocene catalyst and LDPE polymerized under high-pressure process. The space charge in these two samples strongly depended upon the material of electrode. Semicon electrode enhanced carrier injection into LDPEs. The measurements of space charge dynamics revealed the effective mobility of positive and negative carriers. The space charge was much more stable in m-LDPE than in LDPE.journal articl

Ingenuity pathway analysis.

<p>resulting from the 20 top genes given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138223#pone.0138223.t001" target="_blank">Table 1</a> (top genes are in orange).</p

False-positive rate and power.

<p>Results of false-positive rate (a, b, c) and power (d, e, f) are given for the four methods (Rasch, minP, GATES, Fisher and SKAT) across the three scenarios.</p

QQ plots.

<p>resulting from the application of the Rasch-based multi-marker association test to the genes of the Alzheimer ADNI GWAS dataset.</p

Hypothetic signalling network integrating top genes identified through the Rasch analysis.

<p>Gene abbreviations: <i>PLXNC1</i>—plexin C1 receptor for semaphorins; <i>PTK2</i>—<i>FAK</i> kinase implicated in integrin signalling; <i>FYN</i>—src family tyrosine kinase, downstream target of <i>GRM5</i> receptor; <i>EEF2K</i>—eukaryotic elongation factor-2 kinase, activated by <i>GRM5</i> receptor, regulates global protein synthesis; <i>MDM2</i>—negative modulator of <i>TP53</i> tumour suppression gene; <i>RPTOR</i>—regulatory protein associated with <i>MTORC1</i> complex; <i>HDAC3</i>—histone deacetylase; <i>ARC</i>—activity-regulated cytoskeleton-associated protein.</p

Annotation of the 20 top genes resulting from the application of the Rasch-based multi-marker association test to the genes of the Alzheimer ADNI GWAS dataset.

<p>Annotation of the 20 top genes resulting from the application of the Rasch-based multi-marker association test to the genes of the Alzheimer ADNI GWAS dataset.</p

The Right Back Leg of the Woolly Mammoth<i>(M. primigenius)</i> Found in Siberia

<p>The well-preserved mammoth body fragment with foot (33 × 36 cm), shin, and ankle-joint (the total length is ~88 cm) was found in the Enmynveem River valley (north-eastern Siberia, Chukotka). The tissue material (bones, muscles, and skin) had no visible marks of tissue damage by insects or other animals. Radiocarbon dating of the skin and muscle tissue determined that the mammoth lived 32,850 ± 900 y ago [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0040073#pbio-0040073-b012" target="_blank">12</a>].</p