9 research outputs found
Kinetic Studies of Lycopene Isomerization in a Tributyrin Model System at Gastric pH
A semi-preparative HPLC method was developed in order to isolate and purify the 13-cis-lycopene
isomer in tomato-based materials. The result was compared with the naturally predominant all-trans-lycopene isomer, in terms of stability to gastric pH at physiological temperature in a tributyrin model
system. Kinetic experiments confirmed that lycopene isomerization is a reversible reaction, and under
these conditions the all-trans isomer is more stable than the 13-cis isomer. In addition, it was found
that at gastric pH 13-cis-lycopene would predominantly isomerize to the all-trans form rather than
undergo oxidation/breakdown. A simulation based on the rate constants calculated in the kinetic study
indicated that at gastric pH the lycopene isomeric distribution aimed toward an equilibrium
characterized by approx 16% 13-cis-, 16% 9-cis-, and 68% all-trans-lycopene. This study suggests
that pH-driven isomerization in the stomach is at least partially responsible for the relatively high
cis-lycopene proportion found in vivo.
Keywords: Lycopene; cis−trans isomerization; kinetics; gastric pH; tributyri
SDS polyacrylamide gel showing synthetic pleurocidin (Ple) band at appx 3000 kd
<p><b>Copyright information:</b></p><p>Taken from "Recombinant Expression of Pleurocidin cDNA Using
the Expression System"</p><p>Journal of Biomedicine and Biotechnology 2005;2005(4):374-384.</p><p>Published online Jan 2005</p><p>PMCID:PMC1361485.</p><p>Olive-Jean Burrowes et al</p
PCR analysis of transformed cells showing inserted pleurocidin cDNA at 590 bp
<p><b>Copyright information:</b></p><p>Taken from "Recombinant Expression of Pleurocidin cDNA Using
the Expression System"</p><p>Journal of Biomedicine and Biotechnology 2005;2005(4):374-384.</p><p>Published online Jan 2005</p><p>PMCID:PMC1361485.</p><p>Olive-Jean Burrowes et al</p> M represents 500 bp
marker. Lanes 2–5 represent transformed DNA (
KM71H and X-33). Lanes 6–7 represent control DNA (untransformed
cells in lanes) indicated by parrallel arrows. Lanes 8–9
represent vector DNA (vector with and without insert).
Lane 10 represents vector with transformed DNA. Lane 11
represents PCR control (no DNA) indicated by single arrow. Lanes
13–15 represent yeast DNA
Transcribed pleurocidin cDNA bands at 590 b from transformed KMH 71cells at days 5 and 6 (+RT) and gel containing transformed KMH 71 cells (−RT); positive control shown in lane 48
<p><b>Copyright information:</b></p><p>Taken from "Recombinant Expression of Pleurocidin cDNA Using
the Expression System"</p><p>Journal of Biomedicine and Biotechnology 2005;2005(4):374-384.</p><p>Published online Jan 2005</p><p>PMCID:PMC1361485.</p><p>Olive-Jean Burrowes et al</p
Structure and Physical Properties of Zein/Pluronic F127 Composite Films
Triblock copolymer Pluronic F127 has been used to form
composites
with zein, a corn protein and coproduct of the bioethanol industry,
to alleviate its natural brittleness. At low F127 loadings (0–35%),
the plasticizing effect was dominant, and the elongation at break
of zein composite film containing 35% F127 was about 8-fold higher
than that of the zein film with 10% F127. At high F127 loadings (50–100%),
a large number of lamellae crystals were formed in the film matrix
as verified by a combination of differential scanning calorimetry
(DSC), atomic force microscopy (AFM), and small-angle X-ray scattering
(SAXS). The F127 crystallization surpassed its plasticizing effect,
resulting in an increased brittleness of zein film with the further
increase of F127 loading. Compared with the flat-on lamellae of pure
F127, F127 chains folded into branch-like lamellar structures in the
zein composite film containing 50% F127 due to the confinement of
amorphous zein. Besides, the crystals in zein films were composed
of extended chain integral folding (IF = 0) and once-folded chain
(IF = 1) polyethylene oxide (PEO) crystals, and the portion of once-folded
chain (IF = 1) PEO crystals increased with F127 loading. Thus, through
investigation of the competition of plasticizing effect and crystal
formation under different F127 loadings, the optimized F127 loading
in zein/F127 composite film with a good overall performance was determined
to be at around 35%
伝送路の状態を考慮したマルチキャリヤ伝送用回線制御方式
マルチキャリヤ信号のサブキャリヤから伝送路の状態を比較的容易に推定できることに着目し、これを利用して伝送品質を向上させるマルチキャリヤ伝送用の回線制御方式を提案する。提案方式では、受信電力が小さく伝送品質が劣化していると予想されるサブキャリヤにはデータを割り当てずにダミーキャリヤとして送信し、受信電力が大きく伝送品質が良好であると予想されるサブキャリヤのみにデータを割り当てて伝送する。移動通信環境を想定した性能評価の結果、すべてのサブキャリヤにデータを割り当てる通常のマルチキャリヤ伝送方式に比べ、受信レベルの低いサブキャリヤをダミーキャリヤとしてデータを割り当てない提案方式のほうが、伝送効率が向上することを示す。更に提案方式における誤り訂正符号の効果を明らかにする。journal articl
Depiction of Time-dependent Calibration Curve for y-Ray Irradiater Using Radiophotoluminescence and ESR Dosimetry
Time-dependent calibration curve for the γ-ray irradiator was prepared using radiophoto -luminescence (RDPL) and ESR dosimetry. In RDPL measurement, the radiation dose was linearly increased until 38 Gy. However, above 38 Gy, it deviated upward. The ESR signal intensity of γ-irradiated L-α-alanine indicated linear in the range from 0 to 500 Gy. Though ESR signal intensity of L-α-alanine was saturated with the microwave power, the linearity of the calibration curve was reserved at any microwave power (< 5 mW). Summarizing these results, the time-dependent calibration curve was obtained using γ-irradiated L-α-alanine in the range of 0 - 500 Gy after correction by RDPL for the radiation dose below 38 Gy.departmental bulletin pape
