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application/pdf大阪府立大学紀要（人文・社会科学）. 1999, 47, p.41-54departmental bulletin pape

Measurement of the e+e-→D(*)+D(*)- cross sections

journal articl

タシュ セイリ シヒョウ オ モチイタ ロンリテキ シコウ ジョウタイ ノ スイテイ

奈良先端科学技術大学院大学修士(工学)master thesi

Dynamic epigenetic regulation of OsLTP2 gene expression during seed development of rice

application/pdfWe found wound- and pathogen-responsive ex-pression of OsLTP2 gene in leaves of various Tos17 mutants, whose genome were randomly inserted by retrotransposons, and transgenic rice plants during study of stress responses of rice. We assumed that DNA methylation state of the OsLTP2 promoter was altered in a Tos17 mutant line ND7315, because DNA sequence of the OsLTP2 was completely identical to that in wild type, Nipponbare. It was revealed that the OsLTP2 promoter of Nipponbare was highly methylated, whereas the promoter of ND7315 was completely demethylated, by bisulfite sequencing. These results strongly suggest that the demethyla-tion of OsLTP2 promoter cause the alteration of OsLTP2 ex-pression pattern in Tos17 mutants. In Nipponbare, while no expression of OsLTP2 was observed in vegetative organs and spicules at immediately after flowering, strong expression of OsLTP2 was observed in spicules at 5-15 days after flowering. To examine whether dynamic epigenetic alteration of the pro-moter region regulates the stage specific expression of OsLTP2 or not, we investigated the methylation state of OsLTP2 pro-moter at various stages of spicules and seeds. Prominent alter-ation of DNA methylation level in OsLTP2 promoter was ob-served and the level of DNA methylation was greatly correlated with a level of OsLTP2 expression. The methylation of OsLTP2 promoter region was mostly disappeared in 10-15 days after flowering (DAF) developing seeds, and then it was regenerated to original methylation pattern in embryos of dry seeds. These results suggest that the epigenetic alteration of OsLTP2 promoter was programmed in cells of developing seeds.departmental bulletin pape

Pressure and Polymorph Dependent Thermal Decomposition Mechanism of Molecular Materials: A Case of 1,3,5,-Trinitro-1,3,5,-triazine

1,3,5,-Trinitro-1,3,5,-triazine (RDX) serves as an important energetic material and is widely used as various solid propellants and explosives. Understanding the thermal decomposition behaviors of various polymorphs of RDX at high pressure and high temperature is significantly important for safe storage and handling. The present work reveals the early thermal decay mechanisms of two polymorphs (α- and ε-forms) of RDX at high pressure and high temperature by ReaxFF reactive molecular dynamic simulations and climbing image nudged elastic band (CI-NEB) static calculations. It is found that the thermal decomposition rate has positive and negative effects on the pressure for α- and ε-RDX, respectively. This difference originates from the difference of pressure effect on the intermolecular H transfer of the two polymorphs, as we confirm that the bimolecular H transfer rather than the NO2 partition initiates the decay with a significantly lower energy barrier therein. This finding may be beneficial to understand the pressure and polymorph dependent effect on the decay of RDX and to develop a kinetic model for the combustion of solid RDX

Exploring the High-Pressure Polymorphs of Flexible Molecules by Crystal Structure Prediction: A Case of 1,3,5-Trinitro-1,3,5-Triazinane

Exploring the high-pressure polymorphs of energetic compounds is of great interest in evaluating their performances since they always suffer from high pressure in service. We conducted crystal structure prediction (CSP) of 1,3,5-trinitro-1,3,5-triazinane (RDX) at ambient and high pressures by a combined method to emulate energetic crystals in practical use. That is, we first modified a force field (FF) by considering the flexibility of the RDX molecule to primarily screen out the most energetically favored crystal structures under common conditions, and subsequently, the dispersion-corrected density functional theory (DFT-D) method was applied to refine and rank the structures under common and high-pressure conditions. Thereby, all four polymorphs of RDX with experimentally determined crystal structures are reproduced. Apart from the relatively low ranking of β-RDX, α- (1 at 0 GPa), ε-, and γ-RDX (3 and 1 at 5 GPa, respectively) rank very high in the relative energy landscape. The inferiority of the energy ranking of β-RDX suggests a possible kinetic domination in formation. Moreover, a potential high-pressure form, putative η-RDX, is identified as it ranks 1 and 2 in a pressure range of about 2.3 to 3.5 GPa and at 5 GPa, respectively, and needs further experimental verification. Our work validates the modified FF and DFT-D combined method to CSP, whether under pressure loading or not

Morphology Prediction Methods and Their Applications in Energetic Crystals

Crystal morphology can significantly impact rheological, machining, and loading properties of materials and additionally the energy and safety of energetic materials. With the development of computation technology and the increasing requirement of shape-tailored energetic crystals, a high-efficiency morphology prediction method is highly desired. Thus, a timely summary of existing crystal morphology prediction models at the microscopic level and their applications to energetic compounds is implemented in this article to set a basis for comparing their advantages and disadvantages and establishing more accurate ones. In general, these models take into account more structural and thermodynamic factors with fewer or even no kinetic factors, resulting in more or less deviation from experimental observation. Therefore, more accurate morphology prediction models are expected to be constructed in the future, in terms of both macroscopic crystallization conditions and microscopic structures as well as big data

Table_1_Optimal combination of MYCN differential gene and cellular senescence gene predicts adverse outcomes in patients with neuroblastoma.docx

IntroductionNeuroblastoma (NB) is a common extracranial tumor in children and is highly heterogeneous. The factors influencing the prognosis of NB are not simple.MethodsTo investigate the effect of cell senescence on the prognosis of NB and tumor immune microenvironment, 498 samples of NB patients and 307 cellular senescence-related genes were used to construct a prediction signature.ResultsA signature based on six optimal candidate genes (TP53, IL-7, PDGFRA, S100B, DLL3, and TP63) was successfully constructed and proved to have good prognostic ability. Through verification, the signature had more advantages than the gene expression level alone in evaluating prognosis was found. Further T cell phenotype analysis displayed that exhausted phenotype PD-1 and senescence-related phenotype CD244 were highly expressed in CD8+ T cell in MYCN-amplified group with higher risk-score.ConclusionA signature constructed the six MYCN-amplified differential genes and aging-related genes can be used to predict the prognosis of NB better than using each high-risk gene individually and to evaluate immunosuppressed and aging tumor microenvironment.</p

Image_1_Optimal combination of MYCN differential gene and cellular senescence gene predicts adverse outcomes in patients with neuroblastoma.tif

IntroductionNeuroblastoma (NB) is a common extracranial tumor in children and is highly heterogeneous. The factors influencing the prognosis of NB are not simple.MethodsTo investigate the effect of cell senescence on the prognosis of NB and tumor immune microenvironment, 498 samples of NB patients and 307 cellular senescence-related genes were used to construct a prediction signature.ResultsA signature based on six optimal candidate genes (TP53, IL-7, PDGFRA, S100B, DLL3, and TP63) was successfully constructed and proved to have good prognostic ability. Through verification, the signature had more advantages than the gene expression level alone in evaluating prognosis was found. Further T cell phenotype analysis displayed that exhausted phenotype PD-1 and senescence-related phenotype CD244 were highly expressed in CD8+ T cell in MYCN-amplified group with higher risk-score.ConclusionA signature constructed the six MYCN-amplified differential genes and aging-related genes can be used to predict the prognosis of NB better than using each high-risk gene individually and to evaluate immunosuppressed and aging tumor microenvironment.</p

き裂エネルギ密度による安定成長き裂の破壊抵抗評価 (第3報, 薄板延性き裂破壊抵抗の板厚効果)

The influence of sheet thickness on the fracture resistance of a stably growing crack evaluated by crack energy density based on the method proposed in the 1st Report is investigated. That is, the experiments of stable growth fracture similar to those in 2nd Report are carried out varying the sheet thickness, and the fracture resistances expressed by additional rate of crack energy density and crack energy density are evaluated. The results are follows: (1) The fracture resistances are almost independent of initial crack lengths and specimen types for any sheet thickness. (2) With the decrease of sheet thickness, the fracture resistance expressed by crack energy density at the initiation of crack growth decreases and, on the other hand, the fracture resistances expressed by crack energy density and additional rate of crack energy density of stably growing cracks increase under the uniform slant fracture mode. These effects of sheet thickness can be explained by considering the admissible types of plastic deformation around a crack tip.journal articl