Measurement of the e+e-→D(*)+D(*)- cross sections

journal articl

Dynamic epigenetic regulation of OsLTP2 gene expression during seed development of rice

application/pdfWe found wound- and pathogen-responsive ex-pression of OsLTP2 gene in leaves of various Tos17 mutants, whose genome were randomly inserted by retrotransposons, and transgenic rice plants during study of stress responses of rice. We assumed that DNA methylation state of the OsLTP2 promoter was altered in a Tos17 mutant line ND7315, because DNA sequence of the OsLTP2 was completely identical to that in wild type, Nipponbare. It was revealed that the OsLTP2 promoter of Nipponbare was highly methylated, whereas the promoter of ND7315 was completely demethylated, by bisulfite sequencing. These results strongly suggest that the demethyla-tion of OsLTP2 promoter cause the alteration of OsLTP2 ex-pression pattern in Tos17 mutants. In Nipponbare, while no expression of OsLTP2 was observed in vegetative organs and spicules at immediately after flowering, strong expression of OsLTP2 was observed in spicules at 5-15 days after flowering. To examine whether dynamic epigenetic alteration of the pro-moter region regulates the stage specific expression of OsLTP2 or not, we investigated the methylation state of OsLTP2 pro-moter at various stages of spicules and seeds. Prominent alter-ation of DNA methylation level in OsLTP2 promoter was ob-served and the level of DNA methylation was greatly correlated with a level of OsLTP2 expression. The methylation of OsLTP2 promoter region was mostly disappeared in 10-15 days after flowering (DAF) developing seeds, and then it was regenerated to original methylation pattern in embryos of dry seeds. These results suggest that the epigenetic alteration of OsLTP2 promoter was programmed in cells of developing seeds.departmental bulletin pape

Proposal of a Supporting Method for Debugging to Reproduce the Execution of Java Multi-threaded Programs with Petri-Net

It is difficult to implement multi-threaded programs. One of the reason is that the behavior of each thread is non-deterministic. Also it is difficult to reproduce the situation in which an incident occurs. This paper proposes a supporting method for debugging to reproduce Java multi-threaded programs by visualizing the behavior of the programs with Petri-net. Here, it is difficult to express the behavior of multi-threaded programs by ordinal Petri-net. Therefore, We extend Petri-net to reproduce the execution of multi-thread programs to realize our proposal method. Moreover, we have confirmed the effectiveness of our method by implementing a tool which generates a Petri-net model from a multi-threaded program.departmental bulletin pape

き裂エネルギ密度による安定成長き裂の破壊抵抗評価 (第3報, 薄板延性き裂破壊抵抗の板厚効果)

The influence of sheet thickness on the fracture resistance of a stably growing crack evaluated by crack energy density based on the method proposed in the 1st Report is investigated. That is, the experiments of stable growth fracture similar to those in 2nd Report are carried out varying the sheet thickness, and the fracture resistances expressed by additional rate of crack energy density and crack energy density are evaluated. The results are follows: (1) The fracture resistances are almost independent of initial crack lengths and specimen types for any sheet thickness. (2) With the decrease of sheet thickness, the fracture resistance expressed by crack energy density at the initiation of crack growth decreases and, on the other hand, the fracture resistances expressed by crack energy density and additional rate of crack energy density of stably growing cracks increase under the uniform slant fracture mode. These effects of sheet thickness can be explained by considering the admissible types of plastic deformation around a crack tip.journal articl

到来波の偏波特性を利用するアダプティブアレーの特性解析

本論文では， 到来波の偏波特性を利用するアダプティブアレーを提案している． 提案するアダプティブアレーにおいて， その重み係数は， 所望波の偏波に対して直交する偏波成分の電力を最小化することにより決定される． そして， 決定された重み係数を主偏波成分用のアンテナ素子で受信した信号に対して乗算するものである． 本論文では， 提案するアダプティブアレーの構成及び動作原理を示し， 2素子アレーに対して所望波と干渉波の2波が到来している場合の最適ウエート 及びSINRの式を解析的に導出している． 更に， 数値計算により， 本アダプティブアレーの動作特性に対する到来波の偏波特性 及びアンテナ素子の交差偏波識別度の影響を明らかにしている．application/pdfjournal articl

A Transformation Method from ETSC to Timed Petri Net to Improve Matrix-Based Discrete-Event System Controller and its Unified Support System

A sophisticated matrix-based framework “Matrix-Based Discrete-Event System Controller (MDEC)" proposed by Jose Mireles et al is one of the promised method to design control systems of discrete manufacturing systems. In the previous work, we improved MDEC by introducing Timed Petri net and “Expanded Timed-State-Chart" that was developed in our laboratory to describe complex behavior of discrete manufacturing systems (MDEC2). In this work, a computer system supporting to design such control systems was implemented based on MDEC2. ETSC models which users draw through GUI are transformed into Timed Petri nets in the form of matrices; such matrices are embedded into controllers of MDEC2. Through a series of experiments, it was confirmed that obtained controllers could work regularly.departmental bulletin pape

Additional file 2: Table S1. of Integrative genomics analysis of genes with biallelic loss and its relation to the expression of mRNA and micro-RNA in esophageal squamous cell carcinoma

Average tumor:normal mRNA expression fold changes by biallelic loss status for 52 genes in ESCC cases. (XLS 29 kb

Additional file 5: Table S4. of Integrative genomics analysis of genes with biallelic loss and its relation to the expression of mRNA and micro-RNA in esophageal squamous cell carcinoma

Number and frequency of dysregulated miRNAs in ESCC cases. (XLS 31 kb

Comparison of Global Gene Expression of Gastric Cardia and Noncardia Cancers from a High-Risk Population in China

<div><p>Objective</p><p>To profile RNA expression in gastric cancer by anatomic subsites as an initial step in identifying molecular subtypes and providing targets for early detection and therapy.</p><p>Methods</p><p>We performed transcriptome analysis using the Affymetrix GeneChip U133A in gastric cardia adenocarcinomas (n = 62) and gastric noncardia adenocarcinomas (n = 72) and their matched normal tissues from patients in Shanxi Province, and validated selected dysregulated genes with additional RNA studies. Expression of dysregulated genes was also related to survival of cases.</p><p>Results</p><p>Principal Component Analysis showed that samples clustered by tumor vs. normal, anatomic location, and histopathologic features. Paired t-tests of tumor/normal tissues identified 511 genes whose expression was dysregulated (<i>P</i><4.7E-07 and at least two-fold difference in magnitude) in cardia or noncardia gastric cancers, including nearly one-half (n = 239, 47%) dysregulated in both cardia and noncardia, one-fourth dysregulated in cardia only (n = 128, 25%), and about one-fourth in noncardia only (n = 144, 28%). Additional RNA studies confirmed profiling results. Expression was associated with case survival for 20 genes in cardia and 36 genes in noncardia gastric cancers.</p><p>Conclusions</p><p>The dysregulated genes identified here represent a comprehensive starting point for future efforts to understand etiologic heterogeneity, develop diagnostic biomarkers for early detection, and test molecularly-targeted therapies for gastric cancer.</p></div

Additional file 7: Table S6. of Integrative genomics analysis of genes with biallelic loss and its relation to the expression of mRNA and micro-RNA in esophageal squamous cell carcinoma

miRNA and targeted gene tumor:normal expression fold changes (70 miRNAs, 35 genes, 30 ESCC cases). (XLS 30 kb