The Pilgrim's Progress 第一部について : 物語のアレゴリーと語ることのアレゴリー

application/pdf大阪府立大学紀要（人文・社会科学）. 1996, 44, p.33-41departmental bulletin pape

Measurement of the e+e-→D(*)+D(*)- cross sections

journal articl

Cross-Language Information Retrieval Via Dictionary-based and Statistics-based Methods

奈良先端科学技術大学院大学修士(工学)master thesi

Feature Selection and Integration in Automatic Classification of Japanese Texts

application/pdfWe explore the problem of automatic text clas- sification using Japanese documents. Unlike other languages that use roman letters, Japanese language poses a problem of not availing word boundary information. As such bag-of-words approach in constructing features may not be sufficient to en- hance machine learning techniques. We propose a method for feature selection and construction to improve automatic clas- sification performance of Japanese texts. Our approach in- volve extracting syntactic word categories and Chinese charac- ters (Kanji) separately. Then we combine the extracted infor- mation to build an informative feature set. We carried out var- ious experiments using four learning algorithms to evaluate its effectiveness. The proposed method generally outperformed its counterparts method for Japanese document representation.departmental bulletin pape

デザイン学科「全国聾学校・進路に関する調査」結果と考察

departmental bulletin pape

Synthesis, Strong Two-Photon Absorption, and Optical Limiting Properties of Novel C₇₀/C₆₀ Derivatives Containing Various Carbazole Units

An approach was demonstrated toward the design and synthesis of a series of novel C70 and C60 derivatives for large two-photon absorption (TPA). The molecular structures of fullerene derivatives were confirmed by MALDI-MS, 1H NMR, and FT-IR. With femtosecond open-aperture Z-scans and frequency-degenerate pump−probe measurements at 780 nm, the TPA cross sections of up to 3.47 × 10−46, 1.64 × 10−46, 1.1 × 10−46, and 7.82 × 10−47 cm4 s photon−1 molecule−1 were determined for C70-TCTA, C60-TCTA, C70-BCzMB, and C70-MQEtCz in toluene with concentrations of 10−4 M, respectively. The normalized light transmittances of solutions of these molecules were attenuated to the range between 33% and 50% for C70-TCTA, C60-TCTA, C60-BCzMB, and C70-MQEtCz as the input irradiance was increased to about 150 GW/cm2, showing that they are effective optical limiters. Both intensity-dependent Z-scans and pump−probe experiments confirmed that the reduction in the light transmittance results mainly from the TPA process. In addition, the molecule concentration dependence of the TPA cross sections was also investigated. It was found that the TPA cross sections are extremely sensitive to the concentration with the greatest TPA cross-section of 1.0 × 10−45 cm4 s photon−1 molecule−1 for C70-TCTA measured in the low concentration regime (∼10−5 M)

E2F-1 up-regulates TSP1 mRNA expression.

<p>Northern blot analysis of TSP1 expression in 293 cells transfected with HA empty vector of HA-E2F-1 expression vector. β-actin was used as internal control.</p

E2F-1 binding site in TSP1 promoter is required for E2F-1 up-regulation.

<p>A, the partial sequence of TSP1 promoter (−393 to +27bp). Three potential E2F-1 binding sites were marked by boxes and substitution mutations in those sites were indicated underline. B, schematic of eight TSP1 promoter mutation constructs in the region −393 to 0bp. The three potential E2F-1 binding sites were marked by dark circles and mutations were marked by “X”. C, quantification of E2F-1 transcriptional activity on eight TSP1 promoter mutation constructs, 0.2 µg Flag tagged E2F-1/per well was used for transfection in 24-well plates. D, schematic depiction of E2F-1 DNA binding region (amino acid 110–194) cloned into pVP16 vector. E, quantification of E2F-1 transcriptional activity on three TSP1 promoter mutants, 0.2 µg VP-16E2F-1(110–194) or VP-16/per well was used for transfection in 24-well plates. F, quantification of wild-type E2F-1 and E2F-1(E132) transcriptional activity on TSP1 promoter (−2033–0) luciferase reporter, 0.2 µg vector/per well was used for transfection. G, the expression of HA-tagged wild-type E2F-1 and E2F-1 DNA binding site mutant (E132) was verified by Western blot using anti-Flag monoclonal antibody. H, quantification of pRB influence on E2F-1 transcriptional activity on TSP1 promoter (−2033–0) luciferase reporter construct, the expression of HA tagged human pRB was verified by Western blot and the amounts of HA-pRB for transfection were indicated. I, quantification of E2F-1 deletion mutants on TSP1 promoter (−2033–0) luciferase reporter construct. J, the expression of flag-tagged E2F-1 deletion constructs was verified by Western blot using anti-Flag monoclonal antibody, 0.2 µg different construct/per well was used for transfection. K, quantification of E2F-1 siRNA on TSP1 promoter (−2033–0) luciferase reporter activity. L, the knockdown of E2F1 by siRNA in U2OS cells was verified by Western blot using anti-E2F1 monoclonal antibody, a siRNA targeting GFP was used as control, 0.6 µg siRNA expression vector/per well targeting E2F-1 or GFP was used for transfection in 6-well plates.</p

E2F-1 activates TSP1 promoter.

<p>A, schematic depiction of six TSP1 promoter deletion constructs in the region −2033 to +750bp. The transcription initial site is designated as +1. B, quantification of E2F-1 transcriptional activity on six TSP1 promoter constructs. 293 cells were used for assays and 0.2 µg HA tagged E2F-1/per well was used for transfection in 24-well plates. C, dose response of E2F1 on TSP1 promoter activity in U2OS cells. D, the expression of different doses of Flag-tagged E2F1 was verified by Western blot using anti-flag monoclonal antibody, the amounts of Flag-E2F-1 for transfection were indicated. E, the promoter luciferase reporters of E2F1 targeted gene <i>ARF</i>, <i>Apaf1</i> and <i>CyclinE</i> were used as controls to evaluate the transcriptional activity of E2F-1 on TSP1 promoter, 0.2 µg HA tagged E2F-1/per well was used for transfection in 24-well plates. F, schematic depiction of four TSP1 promoter deletion constructs in the region −413 to 0 bp. G, quantification of E2F-1 transcriptional activity on four TSP1 promoter constructs, 0.2 µg HA tagged E2F-1/per well was used for transfection in 24-well plates. H, the expression of HA-tagged E2F-1 was verified by Western blot using anti-HA monoclonal antibody.</p

Inversion of Circularly Polarized Luminescence of Nanofibrous Hydrogels through Co-assembly with Achiral Coumarin Derivatives

Control over the handedness of circularly polarized luminescence (CPL) in supramolecular gels is of special significance in biology and optoelectronics; however, it still remains a great challenge to precisely and efficiently regulate the chirality of CPL. Herein, a chiral phenylalanine-derived hydrogelator and achiral coumarin derivatives can co-assemble into nanofibrous hydrogels with controllable chirality, and the handedness of CPL of these hydrogels can be efficiently inverted by coumarin derivatives through noncovalent interactions, which can be further tuned at will by incorporating metal ions into the co-assembly. The hydrogen bonds, coordination interactions, and steric hindrance are proved to be the crucial factors for the CPL inversion. This study provides feasible strategies to efficiently regulate the handedness of CPL through co-assembly, and these CPL materials may have potential applications in the fields of photoelectric devices, smart chiroptical materials, and biological systems