Biochemical characterization of the Mycobacterium tuberculosis phosphoribosyl-1-pyrophosphate synthetase

Mycobacterium tuberculosis arabinogalactan (AG) is an essential cell wall component. It provides a molecular framework serving to connect peptidoglycan to the outer mycolic acid layer. The biosynthesis of the arabinan domains of AG and lipoarabinomannan (LAM), occurs via a combination of membrane bound arabinofuranosyltransferases, all of which utilise decaprenol-1-monophosphorabinose as a substrate (DPA). The source of arabinose ultimately destined for deposition into cell wall AG or LAM, originates exclusively from phosphoribosyl-1-pyrophosphate (pRpp), a central metabolite which is also required for other essential metabolic processes, such as de novo purine and pyrimidne biosynthesis. In M. tuberculosis, a single pRpp synthetase enzyme (Mt-PrsA) is soley responsible for the generation of pRpp, by catalysing the transfer of pyrophosphate from ATP to the C1 hydroxyl position of ribose-5-phosphate. Here, we report a detailed biochemical and biophysical study of Mt-PrsA, which exhibits the most rapid enzyme kinetics reported for a pRpp synthetase

Elucidation of infection mechanism of Ralstonia solanacearum on ginger

Ehime University (愛媛大学)博士(農学)thesi

High-Frequency Signals Observed in Brain Vessels via Fast Imaging Sequence

Advances in brain imaging technologies have facilitated the determination of functional characteristics of brain areas. However, understanding brain function necessitates exploring signaling processes between brain areas in addition to their functional roles. Functional MRI (fMRI) has been instrumental in identifying brain area functions, yet its temporal resolution limits direct measurement of fast neuronal activity. Recent studies have endeavored to detect fast MRI signals induced by neuronal responses, albeit with limited reproducibility. In this study, we investigated a method proposed by others that achieved fast imaging using short echo and repetition times. Originally applied at 9.4T, we explored the feasibility of implementing this method on 3T scanners, which have lower sensitivity. Our results indicated that the method failed to detect neuronal-origin responses but revealed signals from vessels, suggesting potential insights into brain physiology. Although fast neuronal responses remained undetected, our findings underscore the possibility of characterizing highfrequency signals from vessels, warranting further exploration.journal articl

熱間工具鋼(SKD6)の機械的性質におよぼすオースフォーミングと複合加工熱処理の影響

applicationdepartmental bulletin pape

憲法理論と方法論(一)-とくに戦後における問題提起をめぐって-

Articledepartmental bulletin pape

Summa Conciliorum : summorumque Pontificum a Sancto Petro usque ad Julium Tertium...

Sign.: []4, A-Z4, 2A-2Z4, 3A-3Z4, 4A-4K4, 4L2Digitalización. Vitoria-Gasteiz : Archivos y Bibliotecas, Marzo 199

[Lazarillo de Tormes]

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-201

Summa omniun conciliorum et pontificum

Sign.: a8, A-Z8, 2A-2Z8, 3A-3K8Errores de paginación: de la p. 64 pasa a la p. 89, p. 231-232 y 243-262 repetidas, p. 393 mal numerada, de la p. 400 pasa a la p. 429Port. a dos tintasDigitalización. Vitoria-Gasteiz : Fundación Sancho el Sabio, 2008Digitalización. Vitoria-Gasteiz : Archivos y Bibliotecas, Marzo 1995Carton

Controversia de necessaria residentia personali episcoporu[m] & aliorum inferioru[m] pastoru[m], Tridenti

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 2007Sign.: A-L8Texto con apostillas marginalesPort. con grab. xil