A Message in Commemoration of the Retirement of Professor Yataka Sawaya and Associate Professor Nobuko Takahashi

巻頭言departmental bulletin pape

<Original Article> NMR Spectroscopic Studies on the Urine of Adult Male Mice

To develop a simple and non-invasive method for monitoring the metabolic behavior of experimental animals, NMR spectroscopy was applied to the study of mouse urine constituents. Mice from which urine was obtained were the first male generation hybrid of C57BL/6×C3H at 6 months of age. 1H-NMR spectra at 270 MHz or 400 MHz and 13C-NMR spectra at 100 MHz of the urine (0.3-0.5 ml) were measured by JEOL spectrometers. From the spectra obtained, we have identified citrate and 10 nitrogenous excretion products as urine constituents, in which trimethylammonium and dimethylammonium ions and trimethylamine oxide were confirmed by NMR and mass spectroscopies. The concentrations and the relative ratios of the urine constituents were determined and may serve a criterion for evaluating the metabolic behavior and physiological condition of mice.journal articl

Joint Symposium between Fukui University of Technology and Nation University

The first joint symposium of both university, Fukui University of Technology and Nation University, was held on December 13th and 14th 2014 at the Lampang School of Nation University in Thailand. The joint symposium participants are Tanin Lampang Governor, Suchichai Nation Multimedia Group CEO, Pongin Nation University President, Nation University faculties and Nation University students from Thailand side and Matsuura Director of ASEAN office, Natsulee ASEAN office administrative staff, Horita Assistant Secretary General, Nakayasu Head of International Center, Hashizume Chief of International Center, Nishio Associate Professor, Kigawa Associate Professor, Fujiwara Associate Professor, a grad student and two students of Department of Design from japan side. This joint symposium was published in Thai English-language newspaper "NATION" on the December 15.departmental bulletin pape

Measurement of the branching fractions for B→ωK and B→ωπ

journal articl

Investigation of Buckling Phenomenon Induced by Growth of Vertebral Bodies Using a Mechanical Spine Model

A hypothesis that idiopathic scoliosis is a buckling phenomenon of the fourth or sixth mode, which is the second or third lateral bending mode, induced by the growth of vertebral bodies was presented in a previous paper by the authors using numerical simulations with a finite-element model of the spine. This paper presents experimental proof, of the buckling phenomenon using mechanical spine models constructed with the geometrical data of the finite-element model used in a previous work. Using three spine mechanical models with different materials at intervertebral joints, the change in the natural vibration eigenvalue of the second lateral bending mode with the growth of vertebral bodies was measured by experimental modal analysis. From the result, it was observed that natural vibration eigenvalue decreased with the growth of vertebral bodies. Since the increase in primary factor inducing the buckling phenomenon decreases natural vibration eigenvalue, the obtained result confirms the buckling hypothesis.journal articl

The zebrafish α-tocopherol transfer protein.

<p>A. Alignment of human and zebrafish TTP amino acid sequences is shown. Double dots indicate identical residues and single dots correspond to similar amino acids. Red text signifies α-tocopherol binding pocket. Align2 software (<a href="http://bioinfo.cgrb.oregonstate.edu/fasta2.html" target="_blank">http://bioinfo.cgrb.oregonstate.edu/fasta2.html</a>. Accessed 2012 Sep 17.) was used for sequence comparison. Sequences were obtained from NCBI. B. Anti-human TTP antibody cross-reacts with TTP from adult zebrafish liver homogenate. The 33 kD zebrafish protein (left lane) shown with a Ttp−/− mouse sample as a negative control (right lane) and a WT mouse sample with the 32 kD mouse homolog (left lane).</p

Early morphant malformations.

<p>Images of embryo development from 6–18 hpf demonstrating early effects of TTP knockdown (right panel) compared to an injected control animal at the same age (left panel). Embryos from each MO injection type remain constant through 11 hpf. Beginning at 12 hpf, malformations are noticeable in the rostral region of the TRN embryo. These initial malformations occur in the head at the time the developing eye (marked) becomes distinguishable. The malformations in TRN embryos are more pronounced at later stages of development (16 and 18 hpf), while somite formation continues unabated. Images are frames from a time-lapse video (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047402#pone.0047402.s005" target="_blank">Videos S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047402#pone.0047402.s005" target="_blank">S2</a>).</p

Iosephi Vicecomitis Ambrosiani Colegii Doctoris, Observationes Ecclesiasticae : in quo de Antiquis Baptismi Ritibus, ac caeremonijs agitur : opus sacra eruditione refertum, veterumque Patrum lectioni lumen allaturum.

Mode of access: Internet.Marca tip. en port.Texto con apostillas marginales.Sign.: a4, A-Z8, 2A-2Z8, 3A-3P8, 3Q

TTP expression is dynamic in the developing zebrafish.

<p>A. Embryonic TTP transcription increases during the first 24 hpf. Expression normalized to odc1 expression, and values are expressed as fold change compared to 6 hpf. Data shown as mean ± SEM, 6 hpf n = 4, 9–12 n = 6, 13–18 n = 9, and 19–24 n = 11 replicates (30 embryos per replicate). B-G. Whole mount in situ hybridization of ttpa reveals the patterning of mRNA expression. B. A lateral view of a whole mount embryo at 12 hpf shows fairly even distribution, however, in C a dorsal view of the rostral region with the yolk removed shows specific staining along what may be the developing neural tube. D. At 17 hpf expression remains along the length of the embryo, concentrating in the deeper cells, closer to the yolk sac. E. A dorsal view of the developing head at 17 hpf, the eyes and neural tube is where the expression appears to be localized (outlined). F. By 24 hpf the staining is seen only in the regions of the developing brain, eyes and tail bud. G. Dorsal view depicts brain and eye specific patterning. Yolk sacs were manually removed to reduce color interference, and for ease of positioning. fb = forebrain, mb = midbrain, * = midbrain-hindbrain boundary.</p

Morpholino knockdown of TTP causes severe malformations.

<p>A. MO targeting schematic using a graphic representation of the ttpa transcript. The translational blocking morpholino (TRN) is complementary to the translation start-site, while the splice blocking morpholinos (EXC) bind to the intron/exon junctions on each side of the second exon. Arrows mark primers used to verify aberrant mRNA products resulting from the EXC morpholino (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047402#pone.0047402.s002" target="_blank">Figure S2</a>). Numbered boxes represent exons, and spanning lines are introns, smaller unnumbered boxes are untranslated regions. B. TTP knockdown leads to high incidence of malformation within the first day of development. Data shown as mean percent incidence from seven (TRN, CTR and NON) or three separate experiments (EXC). C. Representative pictures of malformations at 1 dpf due to TTP knockdown. TRN = translational morpholino injected embryo, CTR = standard control injected embryo, concentration and age-matched to the TRN embryo.</p