Pirh2 interacts with and ubiquitylates signal recognition particle receptor β subunit

浜松医科大学博士（医学）浜松医科大学学位論文 医博第506号(平成20年3月17日）doctoral医学系研究科Pirh2 is a RING finger type ubiquitin ligase which ubiquitylates various proteins including p53, p27Kip1, HDAC1, and ε-COP. In this study, we identified signal recognition particle receptor β subunit (SRβ), an integral membrane protein of the endoplasmic reticulum (ER), as a novel Pirh2-interacting protein by yeast two-hybrid screening. We confirmed that Pirh2 interacted with SRβ in mammalian cells. An immunofluorescent staining revealed that Pirh2 colocalized with SRβ in the ER. Pirh2 poly-ubiquitylated SRβ in an intact RING finger domain-dependent manner in vivo and in vitro. Unexpectedly, different from other Pirh2 substrates, neither overexpression of Pirh2 nor depletion of cellular Pirh2 affected SRβ protein stability. Pirh2 preferentially utilized lysine residues 6 and 29 of the ubiquitin to mediate the formation of polyubiquitin chains on SRβ. These results suggest that Pirh2 may regulate SRβ function by mediating poly-ubiquitylation of SRβ without affecting the stability of SRβ protein per se.doctoral thesi

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