V-OPTの開発およびGRINレンズへの応用[ポスター]

開催日時 : 平成17年6月9日、10日 場所 : 理化学研究所　和光研究所　鈴木梅太郎ホールおよび2階ホール　 主催 : 独立行政法人理化学研究所（ＶＣＡＤシステム研究プログラム）textapplication/pdfconference objec

Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts

Background : Tumor necrosis factor (TNF)-α, a proinflammatory cytokine, is involved in the pathogenesis of rheumatoid arthritis (RA). The omega-3 unsaturated fatty acid-derived metabolites resolvin (Rv) D1, RvE1, and maresin-1 (MaR1) have been reported as anti-inflammatory lipid mediators and are known as specialized pro-resolving mediators (SPMs). In this study, we aimed to investigate the anti-inflammatory effects of SPMs on TNF-α-induced responses in synovial fibroblasts. Methods: We investigated the effects of SPMs on gene expression and/or production of cyclooxygenase-2 (COX-2), microsomal prostaglandin E synthase-1 (mPGES-1), interleukin (IL)-6, and matrix metalloproteinase (MMP)-3, which are involved in TNF-α-induced synovitis in RA or OA synovial fibroblasts, by quantitative real-time PCR. We also investigated the effects of SPMs on the mitogen-activated protein kinase (MAPK) signaling pathway by western blotting. Anti-inflammatory effects of SPMs were evaluated by applying SPMs to cultured synovial fibroblasts, followed by TNF-α stimulation. Results: The induction of COX-2, mPGES-1, IL-6, and MMP-3 by TNF-α in synovial fibroblasts was not suppressed by omega 3-derived SPMs regardless of their origin such as RA or OA. SPMs had no effect on lipid mediator receptor gene expression induce by TNF-α and did not inhibit the TNF-α-activated MAPK signaling pathway. The production of COX-2 and IL-6 protein was significantly decreased by p38 inhibitor. Conclusion: Despite reports on the anti-inflammatory effect of omega 3-derived SPMs, its anti-inflammatory effect on TNF-α-induced responses was not observed in synovial fibroblasts. The reason may be that SPMs have no suppressive effect on p38 activation, which plays an important role in the production of inflammatory cytokines in synovial fibroblasts.journal articl

さくら市における地域運営の方向性に関する一考察 ――河戸地区を事例に――

text紀要論文 / Departmental Bulletin Paperdepartmental bulletin pape

Measurement of the B0-B0 mixing rate with B0(B0)→D*∓π± partial reconstruction

journal articl

2007ネンド トショカン ゲンバ エンシュウ ホウコク アサクラシ チュウオウ トショカン イズモ シリツ タイシャ トショカン イバラキシ チュウオウ トショカン ウジシ チュウオウ トショカン オオサカ シリツ チュウオウ トショカン オオサカ フリツ チュウオウ トショカン オオサカ フリツ ナカノシマ トショカン オオタケ シリツ トショカン シガ ケンリツ トショカン センリ コクサイ ガクエン トショカン ドウシシャ ジョシ ダイガク トショ ジョウホウ センター ドウシシャ ジョシ チュウガッコウ コウトウ ガッコウ トショカン ドウシシャ ダイガク ソウゴウ ジョウホウ センター トヤマ シリツ トショカン ナラ ケンリツ トショ ジョウホウカン ヒラカタ シリツ チュウオウ トショカン フクオカ ケンリツ トショカン ヤス トショカン ヤワタ シリツ ヤワタ シミン トショカン リットウ シリツ トショカン

application/pdfdepartmental bulletin pape

カクリツ ブンミャク ジユウ ブンポウ ノ インサイド アウトサイド アルゴリズム ニヨル ガクシュウ ニオケル データ リョウ ノ コウカ

奈良先端科学技術大学院大学修士(工学)master thesi

Head Trauma: Hemorrhage-Iron Deposition

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Stereocontrolled Synthesis of Heterocyclic C-Nucleosides. Protecting Group Effect and Molecular Modeling Studies

We report herein a short stereocontrolled synthesis of heterocyclic C-nucleosides (indole, imidazole, benzimidazole, and 6-iodobenzimidazole). First, condensation of 2-lithiated heterocycles 2−5 with 5-(tert-butyldiphenylsilyl)-2,3-O-isopropylidene-d-γ-ribonolactone (1) afforded the hemiacetals 6−9 in good yields. Then, borohydride reduction (NaBH4) of the protected hemiacetals proceeded stereoselectively to give predominantly the S diols 10−13, which upon Mitsunobu cyclization afforded the α-C-nucleosides 14−17. In contrast, the same PPh3/DEAD treatment of the 1:1 diastereomeric mixture of the free heterocyclic diols 10d and 11d gave exclusively the β-anomers 14dβ and 15dβ, respectively, by a stereocontrolled process. The mechanisms of these stereocontrolled steps are discussed with the support of molecular modeling studies

Stereocontrolled Synthesis of Heterocyclic C-Nucleosides. Protecting Group Effect and Molecular Modeling Studies

We report herein a short stereocontrolled synthesis of heterocyclic C-nucleosides (indole, imidazole, benzimidazole, and 6-iodobenzimidazole). First, condensation of 2-lithiated heterocycles 2−5 with 5-(tert-butyldiphenylsilyl)-2,3-O-isopropylidene-d-γ-ribonolactone (1) afforded the hemiacetals 6−9 in good yields. Then, borohydride reduction (NaBH4) of the protected hemiacetals proceeded stereoselectively to give predominantly the S diols 10−13, which upon Mitsunobu cyclization afforded the α-C-nucleosides 14−17. In contrast, the same PPh3/DEAD treatment of the 1:1 diastereomeric mixture of the free heterocyclic diols 10d and 11d gave exclusively the β-anomers 14dβ and 15dβ, respectively, by a stereocontrolled process. The mechanisms of these stereocontrolled steps are discussed with the support of molecular modeling studies

Synthesis, Incorporation into Triplex-Forming Oligonucleotide, and Binding Properties of a Novel 2‘-Deoxy-<i>C</i>-Nucleoside Featuring a 6-(Thiazolyl-5)benzimidazole Nucleobase

6-(Thiazolyl-5)benzimidazole (Bt) was designed as a novel nucleobase for the specific recognition of an inverted A·T base pair in a triple helix motif. It was successfully incorporated into an 18-mer triplex-forming oligonucleotide (TFO) using the 2‘-deoxy-C-nucleoside phosphoramidite 16. The triple helix binding properties of the modified TFO were examined by means of thermal denaturation experiments targeting an oligopyrimidine·oligopurine 26-mer DNA duplex containing an A·T base pair inversion